Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk

Journal of Dairy Science

Volumn 92, Issue 2, 2009, Pages 491-498

  Chen, W ^a   Chen, H ^a   Xia, Y ^a   Yang, J ^a   Zhao, J ^a   Tian, F ^a   Zhang, H P ^b   Zhang, H ^a  

^a JIANGNAN UNIVERSITY   (China)

^b INNER MONGOLIA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Immobilization; Lactose hydrolysis; Thermostable; galactosidase

Indexed keywords

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 59349095047     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2008-1618     Document Type: Article

References (28)

1. Agrawal, S., S. K. Garg, and S. M. Dutta. 1989. Microbial β-galactosidase: Production, properties and industrial applications - A review. Indian J. Dairy Sci. 42:251-262.
2. Appel, S. H., D. H. Alpers, and G. M. Tomkins. 1965. Multiple molecular forms of β-galactosidase. J. Mol. Biol. 11:12-22.
3. Batra, N., J. Singh, A. Joshi, and R. C. Sobti. 2005. Improved properties of Bacillus coagulans β-galactosidase through immobilization. Eng. Life Sci. 5:581-584.
4. Biro, E., A. S. Nemeth, C. Sisak, T. Feczko, and J. Gyenis. 2008. Preparation of chitosan particles suitable for enzyme immobilization. J. Biochem. Biophys. Methods 70:1240-1246.
5. Bruins, M. E., A. E. Janssen, and R. M. Boom. 2001. Thermozymes and their applications: A review of recent literature and patents. Appl. Biochem. Biotechnol. 90:155-186.
6. Chen, W., H. Chen, Y. Xia, J. Zhao, F. Tian, and H. Zhang. 2008. Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus. J. Dairy Sci. 91:1751-1758.
7. Chen, W., H. Zhang, and X. L. Ding. 2002. Study on properties of thermostable β-galactosidase and hydrolysis of lactose in milk. China Dairy Ind. 30:15-18.
8. Cheng, T. C., K. J. Duan, and D. C. Sheu. 2006. Application of Tris(hydroxymethyl)phosphine as a coupling agent for β-galactosidase immobilized on chitosan to produce galactooligosaccharides. J. Chem. Technol. Biotechnol. 81:233-236.
9. Hirata, H., S. Negoro, and H. Okada. 1985. High production of thermostable β-galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 49:1547-1549.
10. Juers, D. H., T. D. Heightman, A. Vasella, J. D. McCarter, L. Mackenzie, S. G. Withers, and B. W. Matthews. 2001. A structural view of the action of Escherichia coli (lacZ) beta-galactosidase. Biochemistry 40:14781-14794.
11. Juers, D. H., R. H. Jacobson, D. Wigley, X. J. Zhang, R. E. Huber, D. E. Tronrud, and B. W. Matthews. 2000. High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation. Protein Sci. 9:1685-1699.
12. Kang, S. K., K. K. Cho, J. K. Ahn, J. D. Bok, S. H. Kang, J. H. Woo, H. G. Lee, S. K. You, and Y. J. Choi. 2005. Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: Cloning, expression, and enzyme characterization. J. Biotechnol. 116:337-346.
13. Katchalski-Katzir, E. 1993. Immobilized enzymes-learning from past successes and failures. Trends Biotechnol. 11:471-478.
14. Ladero, M., R. Ferrero, A. Vian, A. Santos, and F. Garcia-Ochoa. 2005. Kinetic modelling of the thermal and pH inactivation of a thermostable β-galactosidase from Thermus sp. strain T2. Enzyme Microb. Technol. 37:505-513.
15. Li, X. M., Q. Z. K. Zhou, and X. D. Chen. 2007. Pilot-scale lactose hydrolysis using beta-galactosidase immobilized on cotton fabric. Chem. Eng. Process. 46:497-500.
16. Maciunska, J., M. Scibisz, and J. Synowiecki. 2000. Stability and properties of a thermostable β-galactosidase immobilized on chitin. J. Food Biochem. 24:299-310.
17. Mahoney, R. R. 2003. β-galactosidase. Pages 823-828 in Handbook of Food Enzymology. J. R. Whitaker, ed. Marcel Dekker, New York, NY.
18. Martino, A., M. Durante, P. G. Pifferi, G. Spagna, and G. Bianchi. 1996. Immobilization of fl-glucosidase from a commercial preparation. Part 1.A: comparative study of natural supports. Process Biochem. 3:281-285.
19. Novalin, S., W. Neuhaus, and K. D. Kulbe. 2005. A new innovative process to produce lactose-reduced skim milk. J. Biotechnol. 119:212-218.
20. Oswald, P. R., R. A. Evans, W. Henderson, R. M. Daniel, and C. J. Fee. 1998. Properties of a thermostable β-galactosidase immobilized using tris(hydroxymethyl)phosphine as a highly effective coupling agent. Enzyme Microb. Technol. 23:14-19.
21. Panesar, P. S., R. Panesar, R. S. Singh, J. F. Kennedy, and H. Kumar. 2006. Microbial production, immobilization and applications of β-D-galactosidase. J. Chem. Technol. Biotechnol. 81:530-543.
22. Pessela, B. C., C. Mateo, M. Fuentes, A. Vian, J. L. Garcia, A. V. Carrascosa, J. M. Guisán, and R. Fernández-Lafuente. 2003. The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition: Complete hydrolysis of lactose in dairy products. Enzyme Microb. Technol. 33:199-205.
23. Petzelbauer, I., B. Hidetzeky, D. Haltrich, and K. D. Kulbe. 1999. Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable beta-glycosidases. Biotechnol. Bioeng. 64:322-332.
24. Roy, I., and M. N. Gupta. 2003. Lactose hydrolysis by Lactozym™ immobilized on cellulose beads in batch and fluidized bed modes. Process Biochem. 39:325-332.
25. Shukla, T. P. 1975. Beta-galactosidase technology: A solution to the lactose problem. CRC Crit. Rev. Food Technol. 5:325-356.
26. Stanley, W. L., G. G. Watters, B. Chan, and J. M. Mercer. 1975. Lactose and other enzymes bound to chitin with glutaraldehyde. Biotechnol. Bioeng. 17:315-326.
27. Xia, Y., W. Chen, X. Y. Fu, H. Zhang, S. Yang, and X. L. Ding. 2005. Construction of an integrative food-grade expression system for Bacillus subtilis. Food Res. Int. 38:251-256.
28. Zhou, Q. Z., X. D. Chen, and X. Li. 2003. Kinetics of lactose hydrolysis by Beta-galactosidase of Kluyveromyces lactis immobilized on cotton fabric. Biotechnol. Bioeng. 81:127-133.