Multimodal chromatography: Characterization of protein binding and selectivity enhancement through mobile phase modulators

Journal of Chromatography A

Volumn 1340, Issue , 2014, Pages 151-156

  Wolfe, Leslie S ^a   Barringer, Cartney P ^a   Mostafa, Sigma S ^a   Shukla, Abhinav A ^a  

^a Process Development and Manufacturing KBI Biopharma   (United States)

Author keywords

Host cell protein clearance; Mixed mode chromatography; Monoclonal antibody; Multimodal chromatography; Selective washes

Indexed keywords

BIOCHEMISTRY; MONOCLONAL ANTIBODIES; PHASE MODULATION; PROTEINS; PURIFICATION; RESINS;

CHROMATOGRAPHIC RESINS; HOST CELL PROTEIN; MIXED-MODE CHROMATOGRAPHY; MONOCLONAL ANTIBODY PURIFICATION; MULTIMODAL CHROMATOGRAPHIES; PROTEIN INTERACTION; PURIFICATION PROCESS; SELECTIVITY ENHANCEMENT;

CHROMATOGRAPHY;

MONOCLONAL ANTIBODY; RESIN;

ARTICLE; CATION EXCHANGE; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HYDROPHOBICITY; MATHEMATICAL COMPUTING; NONHUMAN; PH; PHYSICAL PARAMETERS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; SEPARATION TECHNIQUE; STATIC ELECTRICITY;

HOST CELL PROTEIN CLEARANCE; MIXED MODE CHROMATOGRAPHY; MONOCLONAL ANTIBODY; MULTIMODAL CHROMATOGRAPHY; SELECTIVE WASHES;

ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CHROMATOGRAPHY, LIQUID; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MURAMIDASE; PROTEIN BINDING; RIBONUCLEASES; SODIUM CHLORIDE;

EID: 84897958488     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.02.086     Document Type: Article

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