Antibody performance in western blot applications is context-dependent

Biotechnology Journal

Volumn 9, Issue 3, 2014, Pages 435-445

  Älgenäs, Cajsa ^a   Agaton, Charlotta ^b   Fagerberg, Linn ^a   Asplund, Anna ^c   Björling, Lisa ^a   Björling, Erik ^a   Kampf, Caroline ^c   Lundberg, Emma ^a   Nilsson, Peter ^a   Persson, Anja ^a   Wester, Kenneth ^c   Pontén, Fredrik ^c   Wernérus, Henrik ^a   Uhlén, Mathias ^a   Ottosson Takanen, Jenny ^a   Hober, Sophia ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b AlbaNova University Center   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Immunohistochemistry; Monoclonal antibodies; Polyclonal antibodies; Validation; Western blot

Indexed keywords

CELL CULTURE; CHEMICAL REACTIONS; MONOCLONAL ANTIBODIES;

COMPARATIVE STUDIES; CONTEXT DEPENDENT; FULL-LENGTH PROTEINS; IMMUNOHISTOCHEMISTRY; MISLEADING INFORMATIONS; POLYCLONAL ANTIBODY; VALIDATION; WESTERN BLOTS;

PROTEINS;

ESTROGEN RECEPTOR ALPHA; PROTEIN ANTIBODY;

ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; CELL LYSATE; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; PLASMA; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TARGETING; SCORING SYSTEM; TISSUE CULTURE; WESTERN BLOTTING;

IMMUNOHISTOCHEMISTRY; MONOCLONAL ANTIBODIES; POLYCLONAL ANTIBODIES; VALIDATION; WESTERN BLOT;

ANTIBODIES; BLOTTING, WESTERN; CELL LINE; HUMANS; IMMUNOHISTOCHEMISTRY;

EID: 84897641358     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300341     Document Type: Article

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