CHO cell culture longevity and recombinant protein yield are enhanced by depletion of miR-7 activity via sponge decoy vectors

Biotechnology Journal

Volumn 9, Issue 3, 2014, Pages 396-404

  Sanchez, Noelia ^a   Kelly, Paul ^a   Gallagher, Clair ^a   Lao, Nga T ^a   Clarke, Colin ^a   Clynes, Martin ^a   Barron, Niall ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Biopharmaceutical production; Chinese hamster ovary; MiRNA; MiRNA sponge; Target mediated miRNA protection

Indexed keywords

BATCH CELL CULTURE; CLONE CELLS; CLONING; CYTOLOGY; RECOMBINANT PROTEINS; TRANSCRIPTION;

CHINESE HAMSTER OVARY; CHO CELL CULTURES; DEVELOPMENT PROJECT; FED-BATCH CULTURES; GREEN FLUORESCENT PROTEIN; MIRNA; MIRNA SPONGE; RECOMBINANT PROTEIN PRODUCTIONS;

RNA;

GREEN FLUORESCENT PROTEIN; MICRORNA; MICRORNA 7; PROTEIN BCL 10; PROTEIN P53; RECOMBINANT PROTEIN; SMALL INTERFERING RNA; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CHO CELL; CONTROLLED STUDY; FED BATCH CULTURE; FEMALE; IN VITRO STUDY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN SECRETION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA INTERFERENCE; TRANSIENT TRANSFECTION;

CRICETULUS GRISEUS;

BIOPHARMACEUTICAL PRODUCTION; CHINESE HAMSTER OVARY; MIRNA; MIRNA SPONGE; TARGET MEDIATED MIRNA PROTECTION;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; GENETIC VECTORS; LONGEVITY; MICRORNAS; RECOMBINANT PROTEINS;

EID: 84897586906     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300325     Document Type: Article

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