A qPCR assay to detect and quantify shiga toxin-producing E. coli (STEC) in cattle and on farms: A potential predictive tool for STEC culture-positive farms

Toxins

Volumn 6, Issue 4, 2014, Pages 1201-1221

  Verstraete, Karen ^a   Van Coillie, Els ^a   Werbrouck, Hadewig ^a   Van Weyenberg, Stephanie ^a   Herman, Lieve ^a   Del Favero, Jurgen ^b   De Rijk, Peter ^b   De Zutter, Lieven ^c   Joris, Maria Adelheid ^c   Heyndrickx, Marc ^a   De Reu, Koen ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

Author keywords

Cattle; E.Coli; Farm; Feces; Intimin; Isolation; Quantification; Real time PCR; Screening; Shiga toxin

Indexed keywords

SHIGA TOXIN; ADHESIN; BACTERIAL DNA; EAEA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CATTLE; DIAGNOSTIC TEST ACCURACY STUDY; ESCHERICHIA COLI INFECTION; FECES ANALYSIS; GENE; LIMIT OF QUANTITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN GENE 1; SHIGA TOXIN GENE 2; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; THEORY VALIDATION; ANIMAL; BACTERIUM IDENTIFICATION; BOVINAE; CATTLE DISEASES; CLASSIFICATION; DAIRYING; ESCHERICHIA COLI INFECTIONS; FECES; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PREDICTIVE VALUE; VETERINARY;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL TYPING TECHNIQUES; CATTLE; CATTLE DISEASES; DAIRYING; DNA, BACTERIAL; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FECES; FOOD MICROBIOLOGY; PREDICTIVE VALUE OF TESTS; REAL-TIME POLYMERASE CHAIN REACTION; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84897507474     PISSN: 20726651     EISSN: None     Source Type: Journal    
DOI: 10.3390/toxins6041201     Document Type: Article

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