Detection and characterization of verotoxigenic Escherichia coli by a VTEC/EHEC multiplex PCR in porcine faeces and pig carcass swabs

Research in Microbiology

Volumn 154, Issue 2, 2003, Pages 97-104

  Botteldoorn, Nadine ^a   Heyndrickx, Marc ^a   Rijpens, Nancy ^a   Herman, Lieve ^a  

^a Centrum voor Landbouwkundig Onderzoek Gent CLO / Centre de Recherches Agronomiques Gand   (Belgium)

Author keywords

Carcasses; Escherichia coli; ETEC; Farm; Multiplex; Pigs; VTEC

Indexed keywords

ESCHERICHIA COLI ENTEROTOXIN; VEROTOXIN;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CARCASS; CONTROLLED STUDY; ESCHERICHIA COLI; FECES CULTURE; GENE FUNCTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROTYPE; SWINE; SWINE DISEASE; TISSUE CULTURE;

ESCHERICHIA COLI; SUIDAE; SUS SCROFA;

EID: 0037341003     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(03)00028-7     Document Type: Article

References (33)

1. Armstrong G.L., Hollingsworth J., Morris J.M. Jr. Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiol. Rev. 18:1996;29-51.
2. Beutin L., Geier D., Steinruck H., Zimmermann S., Scheutz F. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J. Clin. Microbiol. 31:1993;2483-2488.
3. Beutin L., Geier D., Zimmermann S., Aleksic S., Gillespie H.A., Whittam T.S. Epidemiological relatedness and clonal types of natural populations of Escherichia coli strains producing Shiga toxins in separate populations of cattle and sheep. Appl. Environ. Microbiol. 63:1997;2175-2180.
4. Blanco M., Blanco J.E., Gonzalez E., Mora A., Jansen W., Gomes T., Zerbini F., Yano T., De Castro A., Blanco J. Genes encoding for enterotoxins and verotoxins in porcine Escherichia coli strains belonging to different O:K:H serotypes: Relationship with toxic phenotypes. J. Clin. Microbiol. 35:1997;2958-2963.
5. Bouvet J., Bavai C., Rossel R., Le Roux A., Montet M.P., Ray-Gueniot S., Mazuy C., Arquillière C., Vernory-Rozand C. Prevalence of verotoxin-producing Escherichia coli and E. coli O157:H7 in pig carcasses from three French slaughterhouses. Int. J. Food Microbiol. 71:2001;249-255.
6. Cardeti G., Finazzi G., Tagliabue S., Losio N., Caprioli A., Pacciarini M.L. Detection and characterization of Shiga toxin-producing E. coli (STEC) in different samples from various animal species: One year of experience. Proceedings of the Conference of Pathogenicity and Virulence of VTEC, University of Liège, Belgium, 8-10 November. 1999.
7. Cebula T.A., Payne W.L., Feng P. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol. 33:1995;248-250.
8. Chapman P.A., Siddons C.A., Gerdan Malo A.T., Harkin M.A. A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol. Infect. 119:1997;245-250.
9. DesRosiers A., Fairbrother J.M., Johnson R.P., Desautels C., Letellier A., Quessy S. Phenotypic and genotypic characterization of Escherichia coli verotoxin-producing isolates from humans and pigs. J. Food Protect. 64:2001;1904-1911.
10. 2 ), intimin (eaeA), and enterhemorrhagic E. coli EHEC hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl. Environ. Microbiol. 65:1999;868-872.
11. Fratamico P.M., Bagi L.K., Pepe T. A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. J. Food Protect. 63:2000;1032-1037.
12. Frydendahl K., Ahrens P. Detection of verocytotoxin-producing Escherichia coli in porcine cecal specimens using PCR. Proceedings of the Conference of Pathogenicity and Virulence of VTEC, University of Liège, Belgium, 8-10 November. 1999.
13. Gannon V.P.J., D'Souza S., Graham T., King R.K., Rahn K., Read S. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J. Clin. Microbiol. 35:1997;656-662.
14. Heuvelink A.E., Wernars K., de Boer E. Occurrence of Escherichia coli O157 and other verocytotoxin-producing E. coli in retail raw meats in the Netherlands. J. Food Protect. 59:1996;1267-1272.
15. Heuvelink A.E., Zwartkruis-Nahuis J.T.M., van den Biggelaar F.L.A.M., van Leeuwen W.J., de Boer E. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from slaughter pigs and poultry. Int. J. Food Microbiol. 52:1999;67-75.
16. Hu Y., Zhang Q., Meitzler J.C. Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR. J. Appl. Microbiol. 87:1999;867-876.
17. Johnson W.M., Pollard D.R., Lior H., Tyler S.D., Rozee K.R. Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine endema disease (VTe) by polymerase chain reaction. J. Clin. Microbiol. 28:1990;2351-2353.
18. Karmali M.A., Steele B.T., Petric M., Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet. 1:1983;619-620.
19. Karmali M.A. Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2:1989;15-38.
20. Korsak N., Daube G., Ghafir Y., Chahed A., Jolly S., Vindevogel H. An efficient sampling technique used to detect four foodborne pathogens on pork and beef carcasses in nine Belgian abattoirs. J. Food Protect. 61:1998;535-541.
21. Kuhnert P., Boerlin P., Frey J. Target genes for virulence assessment of Escherichia coli isolates from water, food and the environment. FEMS Microbiol. Rev. 24:2000;107-117.
22. Leung P.H.M., Yam W.C., Ng W.W.S., Peiris J.S.M. The prevalence and characterisation of verotoxin-producing Escherichia coli isolated from cattle and pigs in an abattoir in Hong Kong. Epidemiol. Infect. 126:2001;173-179.
23. O157. J. Clin. Microbiol. 36:1998;598-602.
24. Piérard D., Huyghens L., Lauwers S., Lior H. Diarrhoea associated with Escherichia coli producing porcine oedema disease verotoxin. Lancet. 338:1991;762.
25. Piérard D., Stevens D., Moriau L., Lior H., Lauwers S. Isolation and virulence factors of verocytotoxin-producing Escherichia coli in human stool samples. Clin. Microbiol. Infect. 3:1997;531-540.
26. Piérard D., Van Damme L., Moriau L., Stevens D., Lauwers S. Virulence factors of verocytotoxin-producing Escherichia coli isolated from raw meats. Appl. Environ. Microbiol. 63:1997;4585-4587.
27. Rijpens N., Herman L., Vereecken F., Jannes G., De Smedt J., De Zutter L. Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 46:1999;37-44.
28. Rios M., Prado V., Trucksis M., Arellano C., Borie C., Alexandre M., Fica A., Levine M.M. Clonal diversity of Chilean isolates of enterohemorrhagic Escherichia coli from patients with hemolytic-uremic syndrome, asymptomatic subjects, animal reservoirs, and food products. J. Clin. Microbiol. 37:1999;778-781.
29. - strains that do not produce Shiga toxin: Phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. J. Clin. Microbiol. 37:1999;3491-3496.
30. Smith H.R., Cheasty T., Roberts D., Thomas A., Rowe B. Examination of retail chickens and sausages in Britain for verocytotoxin-producing Escherichia coli. Appl. Environ. Microbiol. 57:1991;2091-2093.
31. Stacy-Phipps S., Mecca J.J., Weiss J.B. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J. Clin. Microbiol. 33:1995;1054-1059.
32. Tsen H.Y., Jian L.Z. Development and use of a multiplex PCR system for the rapid screening of heat-labile toxin I, heat-stable toxine II and Shiga-like toxin I and II genes of Escherichia coli in water. J. Appl. Microbiol. 84:1998;585-592.
33. Tutenel A.V., Houf K., De Zutter L., Uradzinski J., Piérard D., Uyttendaele M., van Hoof J. Voorkomen van enterohemorrhagische coli bij Belgische runderen. Vlaams diergeneeskundig tijdschrift. 69:2000;72-75.