A method for gene amplification and simultaneous deletion in Corynebacterium glutamicum genome without any genetic markers

Plasmid

Volumn 72, Issue 1, 2014, Pages 9-17

  Xu, Jianzhong ^a   Xia, Xiuhua ^b   Zhang, Junlan ^c   Guo, Yanfeng ^a   Qian, He ^a   Zhang, Weiguo ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Inspection Institute Branch of Wuxi   (China)

^c OriGene Biotechnology Co Ltd   (China)

Author keywords

Corynebacterium glutamicum; Double crossover recombination; Gene amplification; Gene deletion; Integration; Marker free

Indexed keywords

ADENYLATE KINASE; ALANINE AMINOTRANSFERASE; DIHYDRODIOL DEHYDROGENASE; FRUCTOSE BISPHOSPHATASE; LYSINE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); GENETIC MARKER;

ALAT GENE; ARTICLE; AVTA GENE; BACTERIAL GENE; BACTERIAL GENOME; CHROMOSOME INSERTION; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DDH GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; FBP GENE; GENE AMPLIFICATION; GENE CASSETTE; GENE DELETION; GENE INSERTION; GENETIC MARKER; HOMOLOGOUS RECOMBINATION; LYSC GENE; NONHUMAN; PCK GENE; RECOMBINANT PLASMID; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; MOLECULAR CLONING; PLASMID;

CLONING, MOLECULAR; CORYNEBACTERIUM GLUTAMICUM; GENE AMPLIFICATION; GENE DELETION; GENES, BACTERIAL; GENETIC ENGINEERING; GENETIC MARKERS; GENOME, BACTERIAL; HOMOLOGOUS RECOMBINATION; LYSINE; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 84897013587     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2014.02.001     Document Type: Article

References (23)

1. Amador E., Franciso J., José M., Castro M. A Brevibacterium lactofermentum16S rRNA gene used as target site for homologous recombination. FEMS Microbiol. Lett. 2000, 185:199-204.
2. Becker J., Zelder O., Häfner S., Schröder H., Wittmann C. From zero to hero-design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production. Metab. Eng. 2011, 13:159-168.
3. Correia A., Martin J.F., Castro J.M. Targeted integration of foreign genes into repetitive sequences of the Brevibacterium lactofermentum genome. FEMS Microbiol. Lett. 1996, 142:259-264.
4. Georgi T., Rittmann D., Wendisch V.F. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: roles of malic enzyme and fructose-1,6-bisphosphatase. Metab. Eng. 2005, 7:291-301.
5. Hou X.H., Chen X.D., Zhang Y., Qian H., Zhang W.G. l-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum. Amino Acids 2012, 43:2301-2311.
6. Ikeda M., Katsumata R. A novel system with positive selection for the chromosomal integration of replicative plasmid DNA in Corynebacterium ghtamicurn. Microbiology 1998, 144:1863-1868.
7. Jakobsen Ø.M., Brautaset T., Degnes K.F., Heggeset T.M.B., Balzer S., Flickinger M.C., Valla S., Ellingsen T.E. Overexpression of wild-type aspartokinase increases l-lysine production in the thermotolerant methylotrophic Bacterium bacillus methanolicus. Appl. Environ. Microbiol. 2009, 75:652-661.
8. Jetten M.S.M., Sinskey A.J. Characterization of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum. FEMS Microbiol. Lett. 1993, 111:183-188.
9. Keilhauer L., Eggeling L., Sahm H. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis the ilvB-ilvN-ilvC operon. J. Bacteriol. 1993, 175:5595-5603.
10. Lu J.H., Liao C.C. Site-directed mutagenesis of the aspartokinase gene lysC and its characterization in Brevibacterium flavum. Lett. Appl. Microbiol. 1997, 24:211-213.
11. Marienhagen J., Eggeling L. Metabolic function of Corynebacterium glutamicum aminotransferases AlaT and AvtA and impact on l-valine production. Appl. Environ. Microbiol. 2008, 74:7457-7462.
12. Ohnishi J., Mitsuhashi S., Hayashi M., Ando S., Yokoi H., Ochiai K., Ikeda M. A novel methodology employing Corynebacterium glutamicum genome information to generate a new l-lysine-producing mutant. Appl. Microbiol. Biotechnol. 2002, 58:217-223.
13. Schäfer A., Tauch A., Jäger W., Kalinowski J., Thierbach G., Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the genome of Corynebacterium glutumicum. Gene 1994, 145:69-73.
14. Srivastava P., Deb J.K. Gene expression systems in corynebacteria. Protein Expr. Purif. 2005, 40:221-229.
15. Tauch A., Götker S., Pühler A., Kalinowski J., Thierbach G. The alanine racemase gene alr is an alternative to antibiotic resistance genes in cloning systems for industrial Corynebacterium glutamicum strains. J. Biotechnol. 2002, 99:79-91.
16. Tauch A., Götker S., Pühler A., Kalinowski J., Thierbach G. Plasmids in Corynebacterium glutamicum and their molecular classification by comparative genomics. J. Biotechnol. 2003, 104:27-40.
17. van der Restá M.E., Lange C., Molenaar D. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 1999, 52:541-545.
18. van Ooyen J., Noack S., Bott M., Reth A., Eggeling L. Improved l-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnol. Bioeng. 2012, 109:2070-2081.
19. Xu D.Q., Tan Y.Z., Huan X.J., Hu X.Q., Wang X.Y. Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J. Microbiol. Methods 2010, 80:86-92.
20. Xu J.Z., Zhang J.L., Guo Y.F., Jia Q.D., Zhang W.G. Heterologous expression of Escherichia coli fructose-1,6-bisphosphatase in Corynebacterium glutamicum and evaluating the effect on cell growth and l-lysine production. Prep. Biochem. Biotechnol. 2014, 44:493-509.
21. Xu J.Z., Zhang J.L., Guo Y.F., Zai Y.G., Zhang W.G. Inprovement of cell growth and production of l-lysine by genetically modified C. glutamicum during growth on molasses. J. Ind. Microbiol. Biotechnol. 2013, 40:1423-1432.
22. Zheng Y.L., Liu M.X., Wang S.H., Zhang Z.X. Fundamental Molecular Biology 2007, Higher Education Press, Beijing, (in Chinese).
23. Zhou J.W., Dong Z.Y., Liu L.M., Du G.C., Chen J. A reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: a practice in Torulopsis glabrata. J. Microbiol. Methods 2009, 76:70-74.