Site-directed mutagenesis of the aspartokinase gene lysC and its characterization in Brevibacterium flavum

Letters in Applied Microbiology

Volumn 24, Issue 3, 1997, Pages 211-213

  Lu, J H ^a   Liao, C C ^a  

^a FOOD INDUSTRY RESEARCH AND DEVELOPMENT INSTITUTE   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENZYMES; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION;

ASPARTOKINASE; BREVIBACTERIUMFLAVUM; ENZYME ASSAYS; MUTANT ENZYMES; OVERLAP EXTENSION POLYMERASE CHAIN REACTIONS; SITE DIRECTED MUTAGENESIS; SPECIFIC ACTIVITY; TRANSFORMANTS; WILD TYPES; WILD-TYPE ENZYMES;

GENES;

ASPARTATE KINASE;

ARTICLE; BREVIBACTERIUM; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

BREVIBACTERIUM; BREVIBACTERIUM FLAVUM; ESCHERICHIA COLI;

EID: 0030889899     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00383.x     Document Type: Article

References (12)

1. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Analytic Biochemistry 72, 248-254.
2. Cremer, J., Eggeling, L. and Sahm, H. (1991) Control of the lysine biosynthesis sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. Applied and Environmental Microbiology 57, 1746-1752.
3. Eikmanns, B.J., Eggeling, L. and Sahm, H. (1993) Molecular aspects of lysine, threonine, and isoleucine biosynthesis in Corynebacterium glutamicum. Antonie van Leeuwenhoek 64, 145-163.
4. Follettie, M.T., Pepples, O.P., Agoropoulou, C. and Sinskey, A.J. (1993) Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon. Journal of Bacteriology 175, 4096-4103.
5. Ho, S.N., Hunt, H.D., Horton, P.M., Pullen, J.K. and Pease, L. (1989) Sire-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59.
6. Kalinowski, J., Bachmann, B., Thierbach, G. and Puhler, A. (1990) Aspartokinase genes lysCα and lysCβ overlap and are adjacent to the aspartate β-semialdehyde dehydrogenase gene asd in Corynebacterium glutamicum. Molecular and General Genetics 224, 317-324.
7. Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H. and Puhler, A. (1991) Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. Molecular Microbiology 5, 1197-1204.
8. Lu, J.-H., Chen, J.L. and Liao, C.-C. (1994) Molecular breeding of a Brevibacterium flavum L-lysine producer using a cloned aspartokinase gene. Biotechnology Letters 16, 449-454.
9. Schrumpf, B., Arzer, A.S., Kalinowski, J., Puhler, A., Effeling, L. and Sahm, H. (1991) A functionally split pathway for lysine synthesis in Corynebacterium glutamicum. Journal of Bacteriology 173, 4510-4516.
10. Thierbach, G., Kalinowski, J., Bachmann, B. and Puhler, A. (1990) Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase. Applied Microbiology and Biotechnology 32, 443-448.
11. Tosaka, O., Takinami, K. and Hirose, Y. (1978) L-Lysine production by S-(2-aminoethyl) L-cysteine and α-amino-β-hydroxyvarelic acid resistant mutants of Brevibacterium lactofermentum. Agricultural and Biological Chemistry 42, 745-752.
12. Yeh, P., Sicard, A.M. and Sinskey, A.J. (1988) General organization of the genes specifically involved in the diaminopimelate-lysine biosynthetic pathway of Corynebacterium glutamicum. Molecular and General Genetics 212, 105-111.