Improvement of cell growth and L-lysine production by genetically modified Corynebacterium glutamicum during growth on molasses

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 12, 2013, Pages 1423-1432

  Xu, Jianzhong ^a   Zhang, Junlan ^b   Guo, Yanfeng ^a   Zai, Yugui ^a   Zhang, Weiguo ^a  

^a JIANGNAN UNIVERSITY   (China)

^b OriGene Biotechnology Co   (China)

Author keywords

Fructokinase; Fructose 1,6 bisphosphatase; Heterologous expression; L Lysine production; Molasses

Indexed keywords

BACTERIAL PROTEIN; DIHYDROXYACETONE; FBP PROTEIN; FRUCTOKINASE; FRUCTOSE; GLUCOSE; GLUCOSE 6 PHOSPHATE; GLYCEROL; LYSINE; MOLASSES; SCRK PROTEIN; SUCROSE; SUGAR; TREHALOSE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CARBON SOURCE; CELL GROWTH; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; GENETIC ENGINEERING; IN VITRO STUDY; PROTEIN EXPRESSION; TRANSGENIC ORGANISM;

CORYNEBACTERIUM GLUTAMICUM;

BETA VULGARIS; CELL PROLIFERATION; CORYNEBACTERIUM GLUTAMICUM; FERMENTATION; FRUCTOKINASES; FRUCTOSE; FRUCTOSE-BISPHOSPHATASE; GLUCOSE; LYSINE; MOLASSES;

EID: 84892787952     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1329-8     Document Type: Article

References (31)

1. Jakobsen ∅M, Brautaset T, Degnes KF, Heggeset TMB, Balzer S, Flickinger MC et al (2009) Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus. Appl Environ Microbiol 75:652-661. doi:10.1128/AEM.01176-08
2. Kaffas M, Stephanopoulos G (2005) Strain improvement by metabolic engineering: Lysine production as a case study for systems biology. Curr Opin Biotechnol 16:361-366. doi:10.1016/j.copbio.2005.04.010
3. Wittmann C, Becker J (2007) The L-lysine story: From metabolic pathways to industrial production. Microbiol Monographs 5:39-70. doi:10.1007/7171-2006- 089
4. Becker J, Klopprogge C, Zelder O, Heinzle E, Wittmann C (2005) Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources. Appl Environ Microbiol 71:8587-8596. doi:10. 1128/AEM.71.12.8587-8596.2005
5. Georgi T, Rittmann D, Wendisch VF (2005) Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6- bisphosphatase. Metab Eng 7:291-301. doi:10.1016/j.ymben. 2005.05.001
6. Lindner SN, Seibold GM, Henrich A, Kramer R, Wendisch VF (2011) Phosphotransferase system (PTS) independent glucose utilization in Corynebacterium glutamicum by inositol permeases and glucokinases. Appl Environ Microbiol 77:3571-3581. doi:10. 1128/AEM.02713-10
7. Dominguez H, Rollin C, Guyonvarch A, Guerquin-Kern JL, Cocaign-Bousquet M, Lindley ND (1998) Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Eur J Biochem 254:96-102. doi:10.1046/j.1432-1327.1998.2540096.x
8. Wittmann C, Kiefer P, Zelder O (2004) Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source. Appl Environ Microbiol 70:7277-7287. doi:10.1128/AEM.70.12.7277-7287.2004
9. Kiefer P, Heinzle E, Zelder O, Wittmann C (2004) Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose. Appl Environ Microbiol 70:229-239. doi:10.1128/AEM.70.1.229-239. 2004
10. Pons A, Dussap CG, Pequinot C, Gros JB (1996) Metabolic flux distribution in Corynebacterium melassecola ATCC 17965 for various carbon sources. Biotechnol Bioeng 51:77-189. doi:10. 1002/(SICI)1097-0290(19960720)51:2\177:AID- BIT7[3.0. CO;2-G
11. Peng X, Okai N, Vertès AA, Inatomi K, Inui M, Yukawa H (2011) Characterization of the mannitol catabolic operon of Corynebacterium glutamicum. Appl Microbiol Biotechnol 91:1375-1387. doi:10.1007/s00253-011-3352-x
12. Kopsahelis N, Nisiotou A, Kourkoutas Y, Panas P, Nycha GJE, Kanellaki M (2009) Molecular characterization and molasses fermentation performance of a wild yeast strain operating in an extremely wide temperature range. Bioresour Technol 100:4854-4862. doi:10.1016/j.biortech.2009.05.011
13. Olbrich H (2006) The molasses. Biotechnologie-Kempe GmbH, Berlin
14. Kopsahelis N, Agouridis N, Bekatorou A, Kanellaki M (2007) Comparative study of spent grains and delignified spent grains as yeast supports for alcohol production from molasses. Bioresour Technol 98:1440-1447. doi:10.1016/j. biortech.2006.03.030
15. Becker J, Klopprogge C, Herold A, Zelder O, Bolten CJ, Wittmann C (2007) Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum - over expression and modification of G6P dehydrogenase. J Biotechnol 138:99-109. doi:10. 1016/j.jbiotec.2007.05.026
16. Becker J, Zelder O, Hafner S, Schroder H, Wittmann C (2011) From zero to hero - design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab Eng 13:159-168. doi:10.1016/j.ymben.2011.01.003
17. Sambrook J, Russel DV (2001) Molecular cloning: A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
18. van der Rest ME, Lange C, Molenaar D (1999) A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl Microbiol Biotechnol 52:541-545
19. Hou XH, Ge XY, Wu D, Qian H, Zhang WG (2012) Improvement of L-valine production at high temperature in Brevibacterium flavum by overexpressing ilvEBNrC genes. J Ind Microbiol Biotechnol 39:63-72. doi:10.1007/s10295-011- 1000-1
20. Porter EV, Chassy BM, Holmlund CE (1980) Partial purification and properties of a mannofructokinase from Streptococcus mutans SL-1. Infect Immun 30:43-50
21. Periyasamy S, Venkatachalam S, Ramasamy S, Srinivasan V (2009) Production of bio-ethanol from sugar molasses using Saccharomyces cerevisiae. Modern Appl Sci 3:32-37
22. Moon MW, Kim HJ, Oh TK, Shin CS, Lee JS, Kim SJ, Lee JK (2005) Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032. FEMS Microbiol Lett 244:259-266. doi:10.1016/j.femsle.2005.01.053
23. Dominguez H, Lingdley ND (1996) Complete sucrose metabolism requires fructose phosphotransferase activity in Corynebacterium glutamicum to ensure phosphorylation of liberated fructose. Appl Environ Microbiol 62:3878-3880
24. Aulkemeyer P, Ebner R, Heilenmann G, Jahreis K, Schmid K, Wrieden S, Lengeler JW (1991) Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria. Mol Microbiol 5:2913-2922. doi:10.1111/j.1365- 2958.1991. tb01851.x
25. Myung S, Wang Y, Zhang YHP (2010) Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: Characterization, metabolite stability, and its implications. Process Biochem 45:1882-1887. doi:10.1016/j.procbio.2010.03. 017
26. Rittmann D, Schaffer S, Wendisch VF, Sahm H (2003) Fructose- 1,6-bisphosphatase from Corynebacterium glutamicum: Expression and deletion of the fbp gene and biochemical characterization of the enzyme. Arch Microbiol 180:285-292. doi:10.1007/s00203-003-0588-6
27. Schaftingen EV, Hers HG (1981) Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Proc Natl Acad Sci USA 78(5):2861-2863
28. Navas MA, Gancedo JM (1996) The regulatory characteristics of yeast fructose-1,6-bisphosphatase confer only a small selective advantage. J Bacteriol 178:1809-1812
29. Ohnishi J, Katahira R, Mitsuhashi S, Kakita S, Ikeda M (2005) A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum. FEMS Microbiol Lett 242:265-274. doi:10.1016/j.femsle.2004.11.014
30. Marx A, Eikmanns BJ, Sahm H, de Graaf AA, Eggeling L (1999) Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase. Metab Eng 1:35-48. doi:10.1006/mben. 1998.0106
31. Xu DQ, Tan YZ, Huan XJ, Hu XQ, Wang XY (2010) Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer. J Microbiol Methods 80:86-92. doi:10.1016/j.mimet.2009.11.003