Osteosarcoma microenvironment: Whole-slide imaging and optimized antigen detection overcome major limitations in immunohistochemical quantification

PLoS ONE

Volumn 9, Issue 3, 2014, Pages

  Kunz, Pierre ^a   Fellenberg, Jörg ^a   Moskovszky, Linda ^b   Sápi, Zoltan ^b   Krenacs, Tibor ^b   Poeschl, Johannes ^a   Lehner, Burkhard ^a   Szendrõi, Miklos ^b   Ewerbeck, Volker ^a   Kinscherf, Ralf ^c   Fritzsching, Benedikt ^a,d  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b SEMMELWEIS UNIVERSITY   (Hungary)

^c PHILIPPS UNIVERSITY MARBURG   (Germany)

^d UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD31 ANTIGEN; CD8 ANTIGEN; TRANSCRIPTION FACTOR FOXP3; TUMOR MARKER;

ANTIGEN DETECTION; ANTIGEN RETRIEVAL; ARTICLE; CONTROLLED STUDY; FALSE NEGATIVE RESULT; HOT SPOT ANALYSIS; HUMAN; HUMAN TISSUE; IMAGING AND DISPLAY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; INTERMETHOD COMPARISON; INVESTIGATIVE PROCEDURES; OSTEOSARCOMA; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; TUMOR MICROENVIRONMENT; WHOLE SLIDE IMAGING;

ANTIGENS, NEOPLASM; DIAGNOSTIC IMAGING; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; KAPLAN-MEIER ESTIMATE; OSTEOSARCOMA; TUMOR MICROENVIRONMENT;

EID: 84896910920     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0090727     Document Type: Article

References (48)

1. Clark JC, Dass CR, Choong PF (2008) A review of clinical and molecular prognostic factors in osteosarcoma. J Cancer Res Clin Oncol 134: 281-297. (Pubitemid 351170066)
2. Klein MJ, Siegal GP (2006) Osteosarcoma: anatomic and histologic variants. Am J Clin Pathol 125: 555-581.
3. Buckanovich RJ, Facciabene A, Kim S, Benencia F, Sasaroli D, et al. (2008) Endothelin B receptor mediates the endothelial barrier to T cell homing to tumors and disables immune therapy. Nat Med 14: 28-36.
4. Curiel TJ, Cheng P, Mottram P, Alvarez X, Moons L, et al. (2004) Dendritic cell subsets differentially regulate angiogenesis in human ovarian cancer. Cancer Res 64: 5535-5538. (Pubitemid 39095544)
5. Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, et al. (2011) Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature 475: 226-230.
6. Gabrilovich DI, Chen HL, Girgis KR, Cunningham HT, Meny GM, et al. (1996) Production of vascular endothelial growth factor by human tumors inhibits the functional maturation of dendritic cells. Nat Med 2: 1096-1103. (Pubitemid 26338628)
7. Osada T, Chong G, Tansik R, Hong T, Spector N, et al. (2008) The effect of anti-VEGF therapy on immature myeloid cell and dendritic cells in cancer patients. Cancer Immunol Immunother 57: 1115-1124.
8. Weidner N, Semple JP, Welch WR, Folkman J (1991) Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N Engl J Med 324: 1-8.
9. Zou W (2005) Immunosuppressive networks in the tumour environment and their therapeutic relevance. Nat Rev Cancer 5: 263-274.
10. Gorlick R, Anderson P, Andrulis I, Arndt C, Beardsley GP, et al. (2003) Biology of childhood osteogenic sarcoma and potential targets for therapeutic development: meeting summary. Clin Cancer Res 9: 5442-5453. (Pubitemid 37499462)
11. Hughes DP (2009) Novel agents in development for pediatric sarcomas. Curr Opin Oncol 21: 332-337.
12. D'Amico F, Skarmoutsou E, Stivala F (2009) State of the art in antigen retrieval for immunohistochemistry. J Immunol Methods 341: 1-18.
13. Fitzgibbons PL, Page DL, Weaver D, Thor AD, Allred DC, et al. (2000) Prognostic factors in breast cancer. College of American Pathologists Consensus Statement 1999. Arch Pathol Lab Med 124: 966-978.
14. Goldstein NS, Hewitt SM, Taylor CR, Yaziji H, Hicks DG (2007) Recommendations for improved standardization of immunohistochemistry. Appl Immunohistochem Mol Morphol 15: 124-133. (Pubitemid 46828218)
15. Hammond ME, Fitzgibbons PL, Compton CC, Grignon DJ, Page DL, et al. (2000) College of American Pathologists Conference XXXV: solid tumor prognostic factors-which, how and so what? Summary document and recommendations for implementation. Cancer Committee and Conference Participants. Arch Pathol Lab Med 124: 958-965. (Pubitemid 30602037)
16. Shi SR, Cote RJ, Yang C, Chen C, Xu HJ, et al. (1996) Development of an optimal protocol for antigen retrieval: a 'test battery' approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J Pathol 179: 347-352. (Pubitemid 26268556)
17. Vermeulen PB, Gasparini G, Fox SB, Colpaert C, Marson LP, et al. (2002) Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. Eur J Cancer 38: 1564-1579. (Pubitemid 34876495)
18. Yamashita S (2007) Heat-induced antigen retrieval: mechanisms and application to histochemistry. Prog Histochem Cytochem 41: 141-200.
19. Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK (1992) A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 40: 787-792.
20. Wilson E, Jackson S, Cruwys S, Kerry P (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods 322: 137-142. (Pubitemid 46560578)
21. Hasegawa T, Hirose T, Kudo E, Hizawa K, Usui M, et al. (1991) Immunophenotypic heterogeneity in osteosarcomas. Hum Pathol 22: 583-590.
22. Balsat C, Blacher S, Signolle N, Beliard A, Munaut C, et al. (2011) Whole slide quantification of stromal lymphatic vessel distribution and peritumoral lymphatic vessel density in early invasive cervical cancer: a method description. ISRN Obstet Gynecol 2011: 354861.
23. Chantrain CF, DeClerck YA, Groshen S, McNamara G (2003) Computerized quantification of tissue vascularization using high-resolution slide scanning of whole tumor sections. J Histochem Cytochem 51: 151-158. (Pubitemid 36160042)
24. Kim NT, Elie N, Plancoulaine B, Herlin P, Coster M (2003) An original approach for quantification of blood vessels on the whole tumour section. Anal Cell Pathol 25: 63-75. (Pubitemid 36443406)
25. van Niekerk CG, van der Laak JA, Borger ME, Huisman HJ, Witjes JA, et al. (2009) Computerized whole slide quantification shows increased microvascular density in pT2 prostate cancer as compared to normal prostate tissue. Prostate 69: 62-69.
26. Kunz P, Hoffend J, Altmann A, Dimitrakopoulou-Strauss A, Koczan D, et al. (2006) Angiopoietin-2 overexpression in morris hepatoma results in increased tumor perfusion and induction of critical angiogenesis-promoting genes. J Nucl Med 47: 1515-1524. (Pubitemid 47544940)
27. Simpson JF, Ahn C, Battifora H, Esteban JM (1996) Endothelial area as a prognostic indicator for invasive breast carcinoma. Cancer 77: 2077-2085. (Pubitemid 26142052)
28. Fox SB, Leek RD, Smith K, Hollyer J, Greenall M, et al. (1994) Tumor angiogenesis in node-negative breast carcinomas-relationship with epidermal growth factor receptor, estrogen receptor, and survival. Breast Cancer Res Treat 29: 109-116. (Pubitemid 24104842)
29. Fritzsching B, Haas J, Konig F, Kunz P, Fritzsching E, et al. (2011) Intracerebral human regulatory T cells: analysis of CD4+ CD25+ FOXP3+ T cells in brain lesions and cerebrospinal fluid of multiple sclerosis patients. PLoS One 6: e17988.
30. Ek ET, Ojaimi J, Kitagawa Y, Choong PF (2006) Outcome of patients with osteosarcoma over 40 years of age: is angiogenesis a marker of survival? Int Semin Surg Oncol 3: 7.
31. Kreuter M, Bieker R, Bielack SS, Auras T, Buerger H, et al. (2004) Prognostic relevance of increased angiogenesis in osteosarcoma. Clin Cancer Res 10: 8531-8537. (Pubitemid 40053420)
32. Mantadakis E, Kim G, Reisch J, McHard K, Maale G, et al. (2001) Lack of prognostic significance of intratumoral angiogenesis in nonmetastatic osteosarcoma. J Pediatr Hematol Oncol 23: 286-289. (Pubitemid 32662036)
33. Mikulic D, Ilic I, Cepulic M, Orlic D, Giljevic JS, et al. (2004) Tumor angiogenesis and outcome in osteosarcoma. Pediatr Hematol Oncol 21: 611-619. (Pubitemid 39336818)
34. Sorensen FB, Jensen K, Vaeth M, Hager H, Funder AM, et al. (2008) Immunohistochemical Estimates of Angiogenesis, Proliferative Activity, p53 Expression, and Multiple Drug Resistance Have No Prognostic Impact in Osteosarcoma: A Comparative Clinicopathological Investigation. Sarcoma 2008: 874075.
35. Shi SR, Cote RJ, Taylor CR (2001) Antigen retrieval techniques: current perspectives. J Histochem Cytochem 49: 931-937. (Pubitemid 32675000)
36. Guo X, Fan Y, Lang R, Gu F, Chen L, et al. (2008) Tumor infiltrating lymphocytes differ in invasive micropapillary carcinoma and medullary carcinoma of breast. Mod Pathol 21: 1101-1107.
37. Kaya M, Wada T, Akatsuka T, Kawaguchi S, Nagoya S, et al. (2000) Vascular endothelial growth factor expression in untreated osteosarcoma is predictive of pulmonary metastasis and poor prognosis. Clin Cancer Res 6: 572-577. (Pubitemid 30111481)
38. Klemke CD, Fritzsching B, Franz B, Kleinmann EV, Oberle N, et al. (2006) Paucity of FOXP3+ cells in skin and peripheral blood distinguishes Sezary syndrome from other cutaneous T-cell lymphomas. Leukemia 20: 1123-1129. (Pubitemid 43797307)
39. Franz B, Fritzsching B, Riehl A, Oberle N, Klemke CD, et al. (2007) Low number of regulatory T cells in skin lesions of patients with cutaneous lupus erythematosus. Arthritis Rheum 56: 1910-1920. (Pubitemid 47110390)
40. Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, et al. (2008) Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Blood 111: 761-766.
41. Schoell WM, Pieber D, Reich O, Lahousen M, Janicek M, et al. (1997) Tumor angiogenesis as a prognostic factor in ovarian carcinoma: quantification of endothelial immunoreactivity by image analysis. Cancer 80: 2257-2262. (Pubitemid 28007396)
42. Mangham DC, Athanasou NA (2011) Guidelines for histopathological specimen examination and diagnostic reporting of primary bone tumours. Clin Sarcoma Res 1: 6.
43. Quintanilla-Martinez L, Tinguely M, Bonzheim I, Fend F (2012) [Bone marrow biopsy: processing and use of molecular techniques]. Pathologe 33: 481-489.
44. Vermeulen PB, Gasparini G, Fox SB, Toi M, Martin L, et al. (1996) Quantification of angiogenesis in solid human tumours: an international consensus on the methodology and criteria of evaluation. Eur J Cancer 32A: 2474-2484.
45. Sullivan CA, Ghosh S, Ocal IT, Camp RL, Rimm DL, et al. (2009) Microvessel area using automated image analysis is reproducible and is associated with prognosis in breast cancer. Hum Pathol 40: 156-165.
46. Fox SB, Leek RD, Weekes MP, Whitehouse RM, Gatter KC, et al. (1995) Quantitation and prognostic value of breast cancer angiogenesis: comparison of microvessel density, Chalkley count, and computer image analysis. J Pathol 177: 275-283.
47. Tomlinson J, Barsky SH, Nelson S, Singer S, Pezeshki B, et al. (1999) Different patterns of angiogenesis in sarcomas and carcinomas. Clin Cancer Res 5: 3516-3522.
48. Xu M, Xu CX, Bi WZ, Song ZG, Jia JP, et al. (2013) Effects of endostar combined multidrug chemotherapy in osteosarcoma. Bone 57: 111-115.