State of the art in antigen retrieval for immunohistochemistry

Journal of Immunological Methods

Volumn 341, Issue 1-2, 2009, Pages 1-18

  D'Amico, Fabio ^a   Skarmoutsou, Evangelia ^a   Stivala, Franca ^a  

^a UNIVERSITY OF CATANIA   (Italy)

Author keywords

Antigen retrieval; Fixation; Immunohistochemistry

Indexed keywords

ALDEHYDE DERIVATIVE; CALCIUM; EDETIC ACID; EGTAZIC ACID; EPITOPE; GLUTARALDEHYDE; METAL ION;

ANTIGEN RETRIEVAL; BLEACHING; CHEMICAL ANALYSIS; ELECTRON MICROSCOPY; ENZYME METABOLISM; HEAT; HIGH TEMPERATURE; IMMUNODETECTION; IMMUNOHISTOCHEMISTRY; IONIC STRENGTH; LOW TEMPERATURE; MICROSCOPY; MICROWAVE RADIATION; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN METABOLISM; REVIEW; ULTRASOUND;

ANIMALS; ANTIGENS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; TISSUE FIXATION;

EID: 59349119394     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.11.007     Document Type: Review

References (273)

1. Allison R.T., and Best T. p53, PCNA and Ki-67 expression in oral squamous cell carcinomas: the vagaries of fixation and microwave enhancement of immunocytochemistry. J. Oral Pathol. Med. 27 (1998) 434
2. Arber D.A. Effect of prolonged formalin fixation on the immunohistochemical reactivity of breast markers. Appl. Immunohistochem. Mol. Morphol. 10 (2002) 183
3. Baas I.O., van den Berg F.M., Mulder J.W., Clement M.J., Slebos R.J., Hamilton S.R., and Offerhaus G.J. Potential false-positive results with antigen enhancement for immunohistochemistry of the p53 gene product in colorectal neoplasms. J. Pathol. 178 (1996) 264
4. Baigent C.L., and Muller G. Carbon-based immunocytochemistry. A new approach to the immunostaining of epoxy-resin-embedded material. J. Microsc. 158 (1990) 73
5. Baker J.R. Cytological technique: the principles underlying routine methods (1966), Chapman and Hall, London
6. Bankfalvi A., Navabli H., Bier B., Bocker W., Jasani B., and Schmid K.W. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. J. Pathol. 174 (1994) 223
7. Battifora H. More on standardization. Appl. Immunohistochem. 6 (1988) 101
8. Battifora H., and Kopinski M. The influence of protease digestion and duration of fixation on the immunostaining of keratins. J. Histochem. Cytochem. 34 (1986) 1095
9. Beckstead J.H. Improved antigen retrieval in formalin-fixed paraffin-embedded tissues. Appl. Immunohistochem. 2 (1994) 274
10. Bendayan M., and Zollinger M. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J. Histochem. Cytochem. 31 (1983) 101
11. Bendayan M., Nanci A., and Kan F.W.K. Effect of tissue processing on colloidal gold cytochemistry. J. Histochem. Cytochem. 35 (1987) 983
12. Berkowitz L.R., Fiorello O., Kruger L., and Maxwell D.S. Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16 (1968) 808
13. Berod A., Hartman B.K., and Pujol J.F. Importance of fixation in immunohistochemistry. Use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase. J. Histochem. Cytochem. 29 (1981) 844
14. Bettica A., and Johnson A.B. Ultrastructural immunogold labeling of glial filaments in osmicated and unosmicated epoxy-embedded tissue. J. Histochem. Cytochem. 38 (1990) 103
15. Boenisch T. Formalin-fixed and heat-retrieved tissue antigens: a comparison of their immunoreactivity in experimental antibody diluents. Appl. Immunohistochem. Mol. Morphol. 9 (2001) 176
16. Boenisch T. Effect of heat-induced antigen retrieval following inconsistent formalin fixation. Appl. Immunohistochem. Mol. Morphol. 13 (2005) 283
17. Boenisch T. Heat-induced antigen retrieval: what are we retrieving?. J. Histochem. Cytochem. 54 (2006) 961
18. Boon M.E., and Kok L.P. Microwaves for immunohistochemistry. Micron 25 (1994) 151
19. Brandtzaeg P. In: Bullock G.R., and Petrusz P. (Eds). Tissue Preparation Methods for Immunohistochemistry. Techniques in immunocytochemistry vol. 1 (1982), Academic Press, London 1
20. Brorson S.H. Improved immunogold labelling of epoxy sections by the use of propylene oxide as additional agent in dehydration, infiltration and embedding. Micron 27 (1996) 345
21. Brorson S.H. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeing on epoxy sections than on LR-White sections for large proteins. Micron 29 (1998) 89
22. Brorson S.H. Heat-induced antigen retrieval of epoxy sections for electron microscopy. Histol. Histopathol. 16 (2001) 923
23. Brorson S.H. Deplasticizing or etching of epoxy sections with different concentrations of sodium ethoxide to enhance the immunogold labelling. Micron 32 (2001) 101
24. Brorson S.H. pH-dependent effect of heat-induced antigen retrieval of epoxy section for electron microscopy. Micron 33 (2002) 481
25. Brorson S.H. A method for measurements of the efficiency of immunogold labelling of epoxy-embedded proteins subjected to different retrieval techniques. Micron 35 (2004) 619
26. Brorson S.H., and Skjørten F. Mechanism for antigen detection on deplasticized epoxy sections. Micron 26 (1995) 301
27. Brorson S.H., and Skjørten F. Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximate the same immunogold labeling for epoxy sections as for acrylic sections without any etching. Micron 27 (1996) 211
28. Brorson S.H., and Nguyen G.H. Increased level of immunogold labeling of epoxy sections by rising the temperature significantly beyond 100 degrees C in the antigen retrieval medium. Micron 32 (2001) 591
29. Brorson S.H., Strøm E.H., and Skjørten F. Immunoelectron microscopy on epoxy sections without deplasticizing to detect glomerular immunoglobulin and complement deposits in renal diseases. APMIS 105 (1997) 139
30. Brorson S.H., Halvorsen I., Lonning L.C., Slaattun G., Sletten M., and Rashid S. Increased yield of immunogold labelling of epoxy sections by adding para-phenylendiamine in the tissue processing. Micron 30 (1999) 561
31. Brorson S.H., Andersen T., Haug S., Kristiansen I., Risstubben A., Tchou H., and Ulstein J. Antigen retrieval on epoxy sections based on tissue infiltration with a moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue. Histol. Histopathol. 14 (1999) 151
32. 2-treated and heated epoxy sections. Micron 32 (2001) 147
33. Brown R.W., and Chirala R. Utility of microwave-citrate antigen retrieval in diagnostic immunohistochemistry. Mod. Pathol. 8 (1995) 515
34. Brown D., Lydon J., McLaughlin M., Stuart-Tilley A., Tyszkowski R., and Alper S. Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS). Histochem. Cell Biol. 105 (1996) 261
35. Cammarata S., Mancardi G., and Tabaton M. Formic acid treatment exposes hidden neurofilament and tau epitopes in abnormal cytoskeletal filaments from patients with progressive supranuclear palsy and Alzheimer's disease. Neurosci. Lett. 115 (1990) 351
36. Cattoretti G., Becker M.H.G., Key G., Duchrow M., Schluter C., Galle J., et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave processed formalin-fixed paraffin sections. J. Pathol. 168 (1992) 357
37. Cattoretti G., Pileri S., Parravicini C., Becker M.H.G., Poggi S., Bifulco C., Key G., D'Amato L., Sabattini E., Feudale E., Reynolds F., Gerdes J., and Rilke F. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171 (1993) 83
38. Causton B.E. The choice of resins for electron immunocytochemistry. In: Polak J.M., and Varndell I.M. (Eds). Immunolabeling for Electron Microscopy (1984), Elsevier, Amsterdam 29
39. Charalambous C., Singh N., and Issacson P.G. Immunohistochemical analysis of Hodgkin's disease using microwave heating. J. Clin. Pathol. 46 (1993) 1085
40. Chiu K.Y. Use of microwave for rapid immunoperoxidase staining of paraffin sections. Med. Lab. Sci. 44 (1987) 3
41. Chu W.S., Furusato B., Wong K., Sesterhenn I.A., Mostofi F.K., Wei M.Q., Zhu Z., Abbondanzo S.L., and Liang Q. Ultrasound-accelerated formalin fixation of tissue improves morphology, antigen and mRNA preservation. Mod. Pathol. 18 (2005) 850
42. Chu W.S., Liang Q., Tang Y., King R., Wong K., Gong M., Wei M., Liu J., Feng S.H., Lo S.C., Andriko J.A., and Orr M. Ultrasound-accelerated tissue fixation/processing achieves superior morphology and macromolecule integrity with storage stability. J. Histochem. Cytochem. 54 (2006) 503
43. Comer M.T., Shires M., Goode N.P., Leese H.J., Trejdosiewicz L.K., and Southgate J. Expression of an antigen associated with basal bodies of human ciliated epithelial cells. Histochem. J. 31 (1999) 39
44. Cuevas E.C., Bateman A.C., Wilkins B.S., Johnson P.A., Williams J.H., Lee A.H.S., Jones D.B., and Wright D.H. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47 (1994) 448
45. Cummings B.J., Mason A.J., Kim R.C., Sheu P.C., and Anderson A.J. Optimization of techniques for the maximal detection and quantification of Alzheimer's-related neuropathology with digital imaging. Neurobiol. Aging 23 (2002) 161
46. Curran R.C., and Gregory J. The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia 33 (1977) 1400
47. D'Alessandro D., Mattii L., Moscato S., Bernardini N., Segnani C., Dolfi A., and Bianchi F. Immunohistochemical demonstration of the small GTPase RhoA on epoxy-resin embedded sections. Micron 35 (2004) 287
48. Dapson R.W. Fixation for the 1990s: a review of needs and accomplishments. Biotech. Histochem. 68 (1993) 75
49. DeArmond S.J., Siegel M.W., Dixon R.G., and Eng L.F. Post-embedding immunoperoxidase staining of glial fibrillary acidic protein for light and electron microscopy. J. Neuroimmunol. 1 (1981) 3
50. De Marzo A.M., Fedor H.H., Gage W.R., and Rubin M.A. Inadequate formalin fixation decreases reliability of p27 immunohistochemical staining: probing optimal fixation time using high-density tissue microarrays. Human Pathol. 33 (2002) 756
51. Dill K.A., and Shortle D. Denatured states of proteins. Annu. Rev. Biochem. 60 (1991) 795
52. Ding M., Robinson J.M., Behrens B.C., and Vandré D.D. The microtubule cytoskeleton in human phagocytic leukocytes is a highly dynamic structure. Eur. J. Cell Biol. 66 (1995) 234
53. Dodson A. Modern methods for diagnostic immunocytochemistry. Curr. Diagn. Pathol. 8 (2002) 113
54. Doi-Yi R., Kitamoto T., and Tateishi J. Immunoreactivity of cerebral amyloidosis is enhanced by protein denaturation treatments. Acta Neuropathol. 82 (1991) 260
55. Duhamel R.C., and Whitehead J.S. Prevention of nonspecific binding of avidin. Meth. Enzymol. 184 (1990) 201
56. Eagle M.E., Mouton S.C., and Emms M. Pediatric rhabdomyosarcoma: MyoD1 demonstration in routinely processed tissue sections using wet heat pretreatment (pressure cooking) for antigen retrieval. J. Clin. Pathol. 50 (1997) 37
57. Elias J.M. Immunohistopathology: a Practical Approach to Diagnosis (1990), ASCP Press, Chicago
58. Eltoum I., Fredenburgh J., and Grizzle W.E. Advanced concepts in fixation: 1. Effects of fixation on immunohistochemistry, reversibility of fixation and recovery of proteins, nucleic acids, and other molecules from fixed and processed tissues. 2. Developmental methods of fixation. J. Histotechnol. 24 (2001) 201
59. Emoto K., Yamashita S., and Okada Y. Mechanisms of Heat-induced Antigen Retrieval: Does pH or Ionic Strength of the Solution Play a Role for Refolding Antigens?. J. Histochem. Cytochem. 53 (2005) 1311
60. Erlandsen S.L., Parsons J.A., and Rodning C.B. Technical parameters of immunostaining of osmicated tissue in epoxy sections. J. Histochem. Cytochem. 27 (1979) 1286
61. Evers P., and Uylings H.B.M. Microwave-stimulated antigen retrieval is pH and temperature dependent. J. Histochem. Cytochem. 42 (1994) 1555
62. Evers P., and Uylings H.B. An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative. J. Neurosci. Methods 72 (1997) 197
63. Evers P., Uylings H.B., and Suurmeijer A.J. Antigen retrieval in formaldehyde-fixed human brain tissue. Methods 15 (1998) 133
64. Ezaki T. Antigen retrieval: its significance and drawbacks in immunohistochemistry. Kaibogaku Zasshi (Acta Anatomica Nipponica) 71 (1996) 615
65. Ezaki T. Antigen retrieval on formaldehyde-fixed paraffin sections: its potential drawbacks and optimization for double immunostaining. Micron 31 (2000) 639
66. Ezaki T., Yao L., and Matsuno K. The identification of proliferatine cell nuclear antigen (PCNA) on rat tissue cryosections and its application to double immunostaining with other markers. Arch. Histol. Cytol. 58 (1995) 103
67. Fossmark R., Martinsen T.C., Qvigstad G., Bendheim M.Ø., Kopstad G., Kashima K., and Waldum H.L. Ultrastructure and chromogranin A immunogold labelling of ECL cell carcinoids. APMIS 113 (2005) 506
68. Foster C.S., Gosden C.M., and Ke Y.Q. Primer: tissue fixation and preservation for optimal molecular analysis of urologic tissues. Nat. Clin. Pract. Urol. 3 (2006) 268
69. Fowler C.B., Cunningham R.E., O Leary T.J., and Mason J.T. 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues. Lab. Invest. 87 (2007) 836
70. Fox C.H., Johnson F.B., Whiting J., and Roller P.P. Formaldehyde fixation. J. Histochem. Cytochem. 33 (1985) 845
71. Fraenkel-Conrat H., and Olcott H.S. The reaction of formaldehydewith proteins. VI. Cross-linking of amino groups with phenol, imidazole or indole groups. J. Biol. Chem. 174 (1948) 827
72. Fraenkel-Conrat H., and Mecham D.K. The reaction of formaldehyde with proteins. VII. Demonstration of intermolecular crosslinking by means of osmotic pressure measurements. J. Biol. Chem. 177 (1949) 477
73. Fraenkel-Conrat H., Brandon B.A., and Olcott H.S. The reaction of formaldehyde with proteins. IV. Participation in indole groups. Gramicidin. J. Biol. Chem. 168 (1947) 99
74. Franciosi S., De Gasperi R., Dickstein D.L., English D.F., Rocher A.B., Janssen W.G., Christoffel D., Sosa M.A., Hof P.R., Buxbaum J.D., and Elder G.A. Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. J. Neurosci. Methods 163 (2007) 76
75. French D., and Edsall J.T. The reaction of formaldehyde with amino acids and proteins. Adv. Protein Chem. 2 (1945) 277
76. Frost A.R., Sparks D., and Grizzle W.E. Methods of antigen recovery vary in their usefulness in unmasking specific antigens in immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 236
77. Furuoka H., Yabuzoe A., Horiuchi M., Tagawa Y., Yokoyama T., Yamakawa Y., Shinagawa M., and Sata T. Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals. Acta Neuropathol. (Berl.) 109 (2005) 263
78. Ganbo T., Sando I., Balaban C.D., Suzuki C., and Sudo M. Immunohistochemistry of lymphocytes and macrophages in human celloidin-embedded temporal bone sections with acute otitis media. Ann. Otol. Rhinol. Laryngol. 106 (1997) 662
79. Goldstein N.S., Hewitt S.M., Taylor C.R., Yaziji H., Hicks D.G., and Members of Ad-Hoc Committee On Immunohistochemistry Standardization. Recommendations for improved standardization of immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 15 (2007) 124
80. Goode N.P., Shires M., Crellin D.M., Khan T.N., and Mooney A.F. Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique. J. Histochem. Cytochem. 52 (2004) 141
81. Gown A.M. Unmasking the mysteries of antigen or epitope retrieval and formalin fixation. Am. J. Clin. Pathol. 121 (2004) 172
82. Griffiths G. Fine structure Immunocytochemistry (1993), Springer-Verlag, New York
83. Groos S., Reale E., and Luciano L. Re-evaluation of epoxy resin sections for light and electron microscopic immunostaining. J. Histochem. Cytochem. 49 (2001) 397
84. Grube D., and Kusumoto Y. Serial semithin sections in immunohistochemistry: techniques and applications. Arch. Histol. Jpn. 49 (1986) 391
85. Guhl B., Ziak M., and Roth J. Unconventional antigen rtrieval for carbohydrate and protein antigens. Histochem. Cell Biol. 110 (1998) 603
86. Gutierrez M., Forster F.I., McConnell S.A., Cassidy J.P., Pollock J.M., and Bryson D.G. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol 71 (1999) 321
87. Habeeb A.J., and Hiramoto R. Reaction of proteins with glutaraldehyde. Arch. Biochem. Biophys. 126 (1968) 16
88. Haerslev T., and Jacobsen G.K. Microwave processing for immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA) in formalin-fixed and paraffin-embedded tissue. APMIS 102 (1994) 395
89. Hammoud M., and Van Noorden S. Failure of ultrasonic vibration as a means of antigen retrieval in routine diagnostic immunocytochemistry. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 249
90. Hand N.M., Blythe D., and Jackson P. Antigen unmasking using microwave heating on formalin fixed tissue embedded in methyl methacrylate. J. Cell. Pathol. 1 (1996) 31
91. Hann C.R., Springett M.J., and Johnson D.H. Antigen retrieval of basement membrane proteins from archival eye tissues. J. Histochem. Cytochem. 49 (2001) 475
92. Haraguchi C.M., and Yokota S. Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues. Histochem. Cell Biol. 117 (2002) 81
93. Haritani M., Spencer Y.I., and Wells G.A. Hydrated autoclave pretreatment enhancement of prion protein immunoreactivity in formalin-fixed bovine spongiform encephalopathy-affected brain. Acta Neuropathol. (Berl.) 87 (1994) 86
94. Hausen P., and Dreyer C. Urea reactivates antigens in paraffin sections for immunofluorescent staining. Stain Technol. 57 (1982) 321
95. Hayat M.A. Chemical fixation. In: Hayat M.A. (Ed). Principles and Techniques of Electron Microscopy (1989), Macmillan Press, London 37
96. Hayat M.A. Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy (2002), Kluwer Academic/Plenum Publisher, New York
97. Helander K.G. Kinetics of formaldehyde binding in tissue. Biotech. Histochem. 69 (1994) 177
98. Ho J., Shintaku I.P., Preston M., and Said J.W. Can microwave antigen retrieval replace frozen-section immunohistochemistry in the phenotyping of lymphoid neoplasms. A comparative study of kappa and lambda light-chain staining in frozen-sections, B5-fixed paraffin sections, and microwave urea antigen retrieval. Appl. Immunohistochem. 2 (1994) 282
99. Hoetelmans R.W.M., van Slooten H.-J., Keijzer R., van de Velde C.J.H., and van Dierdonck J.H. Routine formaldehyde fixation irreversibly reduces immunoreactivity of Bcl-2 in the nuclear compartment of breast cancer cells, but not in the cytoplasm. Appl. Immunohistochem. Mol. Morphol. 9 (2001) 74
100. Holm R., Farrants G.W., Nesland J.M., Sobrinho-Simoes M., Jørgensen O.G., and Johannessen J.V. Ultrastructural and electron immunohistochemical features of medullary thyroid carcinoma. Virchows Arch., A Pathol. Anat. Histopathol. 414 (1989) 375
101. Holund B., and Clemmensen I. The value of hyaluronidase treatment of different tissues before demonstration of fibronectin by the indirect immunoperoxidase technique. Histochemistry 76 (1982) 517
102. Hopwood D., Yeaman G., and Milne G. Differentiating the effects of microwave and heat on tissue proteins and their cross linking by formaldehyde. Histochem. J. 20 (1988) 341
103. Horobin R.W. Histochemistry (1982), Butterworths, London
104. Horobin R.W. Staining plastic section: a review of problems, explanations and possible solutions. J. Microsc. 131 (1983) 173
105. Houen G., and Hansen K. Interference of sugars with the binding of biotin to streptavidin and avidin. J. Immunol. Methods 210 (1997) 115
106. Huang S.N. Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections. Lab. Invest. 33 (1975) 88
107. Huang S.N., Minassian H., and More J.D. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab. Invest. 35 (1976) 383
108. Hunt N.C., Attanoos R., and Jasani B. High temperature antigen retrieval and loss of nuclear morphology: a comparison of microwave and autoclave techniques. J. Clin. Pathol. 49 (1996) 767
109. Igarashi H., Sugimura H., Maruyama K., Kitayama Y., Ohta I., Suzuki M., Tanaka M., Dobashi Y., and Kino I. Alteration of immunoreactivity by hydrated autoclaving, microwave treatment, and simple heating of paraffin-embedded tissue sections. APMIS 102 (1994) 295
110. Ino H. Antigen retrieval by heating en bloc for pre-fixed frozen material. J. Histochem. Cytochem. 51 (2003) 995
111. Itoh H., Miyajima Y., and Osamura R.Y. Immunohistochemistry of intranuclear antigens. Jpn. J. Breast Cancer 10 (1995) 3
112. Iwadare T., Harada E., Yoshino S., and Arai T. A solution for removal of resin from epoxy sections. Stain Technol. 65 (1990) 205
113. Iwamura M., Abrahamsson P.A., Benning C.M., Cockett A.T.K., and di Santagnese P.A. Androgen receptor immunostaining and its tissue distribution in formalin-fixed, paraffin-embedded sections after microwave treatment. J. Histochem. Cytochem. 42 (1994) 783
114. James J.D., and Hauer-Jensen M. Effects of fixative and fixation time for quantitative computerized image analysis of immunohistochemical staining. J. Histotechnol. 22 (1999) 109
115. Jasani B., and Rhodes A. The role and mechanism of high-temperature antigen retrieval in diagnostic pathology. Curr. Diagn. Pathol. 7 (2001) 153
116. Jessie K., Fong M.Y., Devi S., Lam S.K., and Wong K.T. Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization. J. Infect. Dis. 189 (2004) 1411
117. Jiao Y., Sun Z., Lee T., Fusco F.R., Kimble T.D., Meade C.A., Cuthbertson S., and Reiner A. A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections. J. Neurosci. Methods 93 (1999) 149
118. Kakimoto K., Takekoshi S., Miyajima K., and Osamura R.Y. The convincing evidence for heat-induced antigen retrieval mechanism in immunohistochemistry: biochemical and histochemical analysis using dotblotting technique. J. Histochem. Cytochem. 52 (2004) S20
119. Kan R.K., Pleva C.M., Hamilton T.A., and Petrali J.P. Immunolocalization of MAP-2 in routinely formalin-fixed, paraffin-embedded guinea pig brain sections using microwave irradiation: a comparison of different combinations of antibody clones and antigen retrieval buffer solutions. Microsc. Microanal. 11 (2005) 175
120. Kanai K., Nunoya T., Shibuya K., Nakamura T., and Tajima M. Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry. Res. Vet. Sci. 64 (1998) 57
121. Katoh A., and Breier S. Nonspecific antigen retrieval solutions. J. Histotechnol. 17 (1994) 378
122. Kawai K., Serizawa A., Hamana T., and Tsutsumi Y. Heat-induced antigen retrieval of proliferating cell nuclear antigen and p53 protein in formalin-fixed, paraffin-embedded sections. Pathol. Int. 44 (1994) 759
123. Kellenberger E., Dürrenberger M., Villiger W., Carlemalm E., and Wurtz M. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J. Histochem. Cytochem. 35 (1987) 959
124. Kim S.H., Jung K.C., Shin Y.K., Lee K.M., Park Y.S., Choi Y.L., Oh K.I., Kim M.K., Chung D.H., Song H.G., and Park S.H. The enhanced reactivity of endogenous biotin-like molecules by antigen retrieval procedures and signal amplification with tyramine. Histochem. J. 34 (2002) 97
125. Kim S.H., Kook M.C., and Song H.G. Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues. J. Mol. Histol. 35 (2004) 403
126. Kim S.H., Kook M.C., Shin Y.K., Park S.H., and Song H.G. Evaluation of antigen retrieval buffer systems. J. Mol. Histol. 35 (2004) 409
127. Kitamoto T., Ogomori K., Tatesiner J., and Prusiner S.B. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab. Invest. 57 (1987) 230
128. Kodama T., Kawamoto K., Kono T., Shibuya Y., and Setogawa T. Comparison of immunostainings for proliferating cell nuclear antigen and Ki-67 in human extraocular lesions. Graefe's Arch. Clin. Exp. Ophthalmol. 235 (1997) 767
129. Koopal S.A., Coma M.I., Tiebosch A.T.M.G., and Suurmeijer A.J.H. Low-temperature heating overnight in ]Tris-HCl buffer pH 9 is a good alternative for antigen retrieval in formalin-fixed paraffin-embedded tissue. Appl. Immunohistochem. 6 (1998) 228
130. Kosaka T., Nagatsu I., Wu J.Y., and Hama K. Use of high concentrations of glutaraldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system. Neuroscience 18 (1986) 975
131. Kunkel G.R., Mehrabian M., and Martinson H.G. Contact-site cross-linking agents. Mol. Cell. Biochem. 34 (1981) 3
132. Lane B.P., and Europa D.L. Differential staining of ultrathin sections of epon embedded tissues for light microscopy. J. Histochem. Cytochem. 13 (1965) 579
133. Larsson L.I. Immunocytochemistry: theory and practice (1988), CRC Press, Boca Raton
134. Larsson L.I. Tissue preparation methods for light microscopic immunohistochemistry. Appl. Immunohistochem. 1 (1993) 2
135. Lee H., Douglas-Jones A.G., Morgan J.M., and Jasani B. The effect of fixation and processing on the sensitivity of oestrogen receptor assay by immunohistochemistry in breast carcinoma. J. Clin. Pathol. 55 (2002) 236
136. Leenen P.J.M., Jansen A., and Van Ewijk W. In: Bullock G.R., and Petrusz P. (Eds). Fixation Parameters for Immunocytochemistry: the Effect of Glutaraldehyde or Paraformaldehyde Fixation on the Preservation of Mononuclear Phagocyte Differentiation Antigens. Techniques in Immunocytochemistry vol. 3 (1985), Academic Press, London
137. Leong A.S., and Gilham P.N. The effects of progressive formaldehyde fixation on the preservation of tissue antigens. Pathology 21 (1989) 266
138. Leong A.S., and Sormunen R.T. Microwave procedures for electron microscopy and resin embedded sections. Micron 29 (1998) 397
139. Leong A.S., and Leong T.Y. Newer developments in immunohistology. J. Clin. Pathol. 59 (2006) 1117
140. Leong T.Y., and Leong A.S. How does antigen retrieval work?. Adv. Anat. Pathol. 14 (2007) 129
141. Leong A.S., Lee E.S., Yin H., Kear M., Haffajee Z., and Pepperall D. Superheating antigen retrieval. Appl. Immunohistochem. Mol. Morphol. 10 (2002) 263
142. Litwin J.A., Yokota S., Hashimoto T., and Fahimi H.D. Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections. Histochemistry 81 (1984) 15
143. Litwin J.A., Volkl A., Muller-Hocker J., Hashimoto T., and Fahimi H.D. Immunocytochemical localization of peroxisomal enzymes in human liver biopsies. Am. J. Pathol. 128 (1987) 141
144. Lobo M.V., Alonso F.J., Arenas M.I., Caso E., Fraile B., and del Rio R.M. Ultrastructural staining with sodium metaperiodate and sodium borohydride. J. Histochem. Cytochem. 50 (2002) 11
145. Long II D.J., and Buggs C. Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues. J. Mol. Histol. 39 (2008) 1
146. Lucassen P.J., Ravid R., Gonatas N.K., and Swaab D.F. Activation of the human supraoptic and paraventricular nucleus neurons with aging and in Alzheimers disease as judged from increasing size of the Golgi apparatus. Brain Res. 632 (1993) 105
147. Lukinmaa P.L., and Waltimo J. Immunohistochemical localization of types I, V, and VI collagen in human permanent teeth and periodontal ligament. J. Dent. Res. 71 (1992) 391
148. Malisius R., Merz H., Heinz B., Gafumbegete E., Koch B.U., and Feller A.C. Constant detection of CD2, CD3, CD4, and CD5 in fixed and paraffin-embedded tissue using the peroxidase-mediated deposition of biotin-tyramide. J. Histochem. Cytochem. 45 (1997) 1665
149. Mar H., and Wight T.N. Colloidal gold immunostaining on deplasticized ultra-thin sections. J. Histochem. Cytochem. 36 (1988) 1387
150. Mason J.T., and O Leary T.J. Effect of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J. Histochem. Cytochem. 39 (1991) 225
151. Maxwell. Two rapid and simple methods used for the removal of resins from 1.0 μm thick epoxy sections. J. Microsc. 112 (1978) 253
152. Maxwell P., and McCluggage W.G. Audit and internal quality control in immunohistochemistry. J. Clin. Pathol. 53 (2000) 929
153. Mayor H.D., Hampton J.C., and Rosario B. A simple method for removing the resin from epoxy-embedded tissue. J. Biophys. Biochem. Cytol. 9 (1961) 909
154. McCluggage W.G., Roddy S., Whiteside C., Burton J., McBride H., Maxwell P., and Bharucha H. Immunohistochemical staining of plastic embedded bone marrow trephine biopsy specimens after microwave heating. J. Clin. Pathol. 48 (1995) 840
155. McGhee J.D., and von Hippel P.H. Formaldehyde as a probe of DNA structure. Mechanism of the initial reaction of formaldehyde with DNA. Biochemistry 16 (1977) 3276
156. McNicol A.M., and Richmond J.A. Optimizing immunohistochemistry:antigen retrieval and signal amplification. Histopathology 32 (1998) 97
157. McQuaid S., McConnell R., McMahon J., and Herron B. Microwave antigen retrieval for immunocytochemistry on formalin-fixed, paraffin-embedded post-mortem CNS tissue. J. Pathol. 176 (1995) 207
158. Mepham B.L., Frater W., and Mitchell B.S. The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique. Histochem. J. 11 (1979) 345
159. Merighi A. Post-embedding electron microscopic immunocytochemistry. In: Polak J.M., and Priestley J.V. (Eds). Electron Microscopic Immunocytochemistry (1992), Oxford University Press, Oxford 51
160. Merighi A., and Polak J.M. Post-embedding immunogold staining. In: Cuello A.C. (Ed). Immunohistochemistry II (1993), Wiley, New York 229
161. Merriam R.W. The contribution of lower oxides of osmium to the density of biological specimens in electron microscopy. J. Biophys. Biochem. Cytol. 4 (1958) 579
162. Merz H., Rickers O., Schrimel S., Orscheschek K., and Feller A.C. Constant detection of surface and cytoplasmic immunoglobulin heavy and light chain expression in formalin fixed and paraffin embedded material. J. Pathol. 170 (1993) 257
163. Metz B., Kersten F., Hoogerhout P., Brugghe H., Timmermans H., de Jong A., Meiring H., ten Hove J., Hennink W., Crommelin D., and Jiskoot W. Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J. Biol. Chem. 279 (2004) 6235
164. Metz B., Kersten G.F., Baart G.J., de Jong A., Meiring H., ten Hove J., van Steenbergen M.J., Hennink W.E., Crommelin D.J., and Jiskoot W. Identification of formaldehyde-induced modifications in proteins: reactions with insulin. Bioconjug. Chem. 17 (2006) 815
165. Miller R.T., Swanson P.E., and Wick M.R. Fixation and epitope retrieval in diagnostic immunohistochemistry: a concise review with practical considerations. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 228
166. Miller M.A., Ramos-Vara J.A., Kleiboeker S.B., and Larson R.L. Effects of delayed or prolonged fixation on immunohistochemical detection of bovine viral diarrhea virus type I in skin of two persistently infected calves. J. Vet. Diagn. Invest. 17 (2005) 461
167. Montero C., Chi-Ahumada E., Chavez-Porras S., and Gutierrez M. Application of the critical molar concentration concept to heat-mediated antigen retrieval in immunohistochemistry. Histochem. J. 32 (2000) 111
168. Morgan J.M., Navabi H., Schmid K.W., and Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high temperature antigen retrieval. J. Pathol. 174 (1994) 301
169. Morgan J.M., Navabi H., and Jasani B. Role of calcium chelation in high-temperature antigen retrieval at different pH values. J. Pathol. 182 (1997) 233
170. Moriarty G.C. Adenohypophysis: ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21 (1973) 855
171. Mount S.L., and Cooper K. Beware of biotin: a source of false-positive immunohistochemistry. Curr. Diagn. Pathol. 7 (2001) 161
172. Mukai M., Torikata C., Iri H., Hata J., Naito M., and Shimoda T. Immunohistochemical identification of aggregated actin filaments in formalin-fixed, paraffin-embedded sections. I. A study of infantile digital fibromatosis by a new pretreatment. Am. J. Surg. Pathol. 16 (1992) 110
173. Munakata S., and Hendricks J.B. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue. J. Histochem. Cytochem. 41 (1993) 1241
174. Namimatsu S., Ghazizadeh M., and Sugisaki Y. Reversing the effects of formalin fixation with citraconic anhydride and heat: a universal antigen retrieval method. J. Histochem. Cytochem. 53 (2005) 3
175. Newman G.R. LR-White embedding medium for colloidal gold methods. In: Hayat M.A. (Ed). Colloidal Gold: Principles, Methods and Applications (1989), Academic Press, New York 47
176. Norton A.J., Jordan S., and Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J. Pathol. 173 (1994) 371
177. Nyhlin N., el-Salhy M., Sandstrom O., and Suhr O. Evaluation of immunohistochemical staining of human duodenal endocrine cells after microwave antigen retrieval. Histochem. J. 29 (1997) 177
178. Ohara K., Shin M., Larsson L.I., and Fujiwara K. Improved immunocytochemical detection of daunomycin. Histochem. Cell Biol. 127 (2007) 603
179. O'Leary T.J. Standardization in immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 9 (2001) 3
180. Ordonez N.G., Manning J.T., and Brooks T.E. Effect of trypsinisation on the immunostaining of formalin-fixed paraffin-embedded tissues. Am. J. Surg. Pathol. 12 (1988) 121
181. Ottersen O.P. Quantitative electron microscopical immunocytochemistry of neuroactive aminoacids. Anat. Embryol. 180 (1989) 1
182. Pearse A.G.E. Histochemistry: Theoretical and Applied vol. 1 (1980), Churchill Livingstone, Edinburgh
183. Peranen J., Rikkonen M., and Kaariainen L. A method for exposing hidden antigenic sites in paraformaldehyde-fixed cultured cells, applied to initially unreactive antibodies. J. Histochem. Cytochem. 41 (1993) 447
184. Peston D., and Shousha S. Low temperature heat mediated antigen retrieval for demonstration of oestrogen and progesterone receptors in formalin-fixed paraffin sections. J. Cell. Pathol. 3 (1998) 91
185. Pfeiffer S.W. Use of hydrogen peroxide to accelerate staining of ultrathin spurr sections. Stain Technol. 57 (1982) 137
186. Pileri S.A., Roncador G., Ceccarelli C., Piccioli M., Briskomatis A., Sabattini E., Ascani S., Santini D., Piccaluga P.P., Leone O., Damiani S., Ercolessi C., Sandri F., Pieri F., Leoncini L., and Falini B. Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J. Pathol. 183 (1997) 116
187. Pillai G., Roberts H., Gatter K., and Pezzella F. p53 expression in normal paraffin-embedded tissue using different antibodies and antigen retrieval buffer systems. Histopathology 42 (2003) 83
188. Podkletnova I., and Alho H. Ultrasound-amplified immunohistochemistry. J. Histochem. Cytochem. 41 (1993) 51
189. Porcelli M., Cacciapuoti G., Fusco S., Massa R., d Ambrosio G., Bertoldo C., De Roas M., and Zappia V. Non-thermal effects of microwaves on proteins: thermophilic enzymes as model system. FEBS Lett. 402 (1997) 102
190. Priestley J.V., Alvarez F.J., and Averill S. Pre-embedding electron microscopic immunocytochemistry. In: Polak J.M., and Priestley J.V. (Eds). Electron Microscopic Immunocytochemistry (1992), Oxford University Press, Oxford 89
191. Privat N., Sazdovitch V., Seilhean D., Laplanche J.L., and Hauw J.J. PrP immunohistochemistry: different protocols, including a procedure for long formalin fixation, and a proposed schematic classification for deposits in sporadic Creutzfeldt-Jacob disease. Microsc. Res. Tech. 50 (2000) 26
192. Puchtler H., and Meloan S.N. On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Histochemistry 82 (1985) 201
193. Rait V.K., O Leary T.J., and Mason J.T. Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I-Structural and functional alterations. Lab. Invest. 84 (2004) 292
194. Rajamannan N.M., Springett M.J., Pederson L.G., and Carmichael S.W. Localization of caveolin 1 in aortic valve endothelial cells using antigen retrieval. J. Histochem. Cytochem. 50 (2002) 617
195. Rangell L.K., and Keller G.A. Application of microwave technology to the processing and immunolabeling of plastic-embedded and cryosections. J. Histochem. Cytochem. 48 (2000) 1153
196. Rhodes A. Quality assurance in immunohistochemistry. Am. J. Surg. Pathol. 27 (2003) 1284
197. Robinson J.M., and Vandré D.D. Efficient immunocytochemical labeling of leukocyte microtubules with fluoronanogold: an important tool for correlative microscopy. J. Histochem. Cytochem. 45 (1997) 631
198. Robinson J.M., and Vandré D.D. Antigen retrieval in cells and tissues: enhancement with sodium dodecyl sulphate. Histochem. Cell Biol. 116 (2001) 119
199. Röcken C., and Roessner A. An evaluation of antigen retrieval procedures for immunoelectron microscopic classification of amyloid deposits. J. Histochem. Cytochem. 47 (1999) 1385
200. Rojo F., Tabernero J., Albanell J., Van Cutsem E., Ohtsu A., Doi T., Koizumi W., Shirao K., Takiuchi H., Ramon y Cajal S., and Baselga J. Pharmacodynamic studies of gefitinib in tumor biopsy specimens from patients with advanced gastric carcinoma. J. Clin. Oncol. 24 (2006) 4309
201. Roth J. The protein A-gold (pAg) technique. A qualitative and quantitative approach for antigen localization on thin sections. In: Bullock G.R., and Petrusz P. (Eds). Techniques in immunocytochemistry (1982), Academic Press, London 107
202. Roth J. Post-embedding cytochemistry with gold-labelled reagents: a review. J. Microsc. 143 (1986) 125
203. Sabit H., Tsuneyama K., Shimonishi T., Harada K., Cheng J., Ida H., Saku T., Saito K., and Nakunuma Y. Enhanced expression of basement membrane-type heparan sulfate proteoglycan in tumor fibro-myxoid stroma of intrahepatic cholangiocarcinoma. Pathol. Int. 51 (2001) 248
204. Saito N., Takemori N., Hirai K., Kubota N., Onodera R., and Namiki M. Application of immunocryoultramicrotomy to free cells. Am. J. Hematol. 39 (1992) 223
205. Saito N., Konishi K., Takeda H., Kato M., Sugiyama T., and Asaka M. Antigen retrieval trial for post-embedding immunoelectron microscopy by heating with several unmasking solutions. J. Histochem. Cytochem. 51 (2003) 989
206. Schwendemann G., Wolinski J.S., Hatzidimitriou G., Merz D.C., and Waxham M.N. Postembedding immunocytochemical localization of paramyxovirus antigens by light and electron microscopy. J. Histochem. Cytochem. 30 (1982) 1313
207. Seidal T., Balaton A.J., and Battifora H. Interpretation and quantification of immunostains. Am. J. Surg. Pathol. 25 (2001) 1204
208. Sheriffs I.N., Rampling D., and Smith V.V. Paraffin wax embedded muscle is suitable for the diagnosis of muscular dystrophy. J. Clin. Pathol. 54 (2001) 517
209. Shi S.R., and Tian Q. Development of an antigen retrieval technique for immunohistochemistry on archival celloidin-embedded sections. J. Histochem. Cytochem. 41 (1993) 1121
210. Shi S.R., Key M.E., and Kalra K.L. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave heating of tissue sections. J. Histochem. Cytochem. 39 (1991) 741
211. Shi S.R., Coté C., Kalra K.L., Taylor C.R., and Tandon A.K. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J. Histochem. Cytochem. 40 (1992) 787
212. Shi S.R., Chaiwun B., Young L., Cote R.J., and Taylor C.R. Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J. Histochem. Cytochem. 41 (1993) 1599
213. Shi S.R., Gu J., Kalra K.L., Chen T., Cote R.J., and Taylor C.R. Antigen retrieval technique: A novel approach to immunohistochemistry on routinely processed tissue sections. Cell Vis. 2 (1995) 6
214. Shi S.R., Imam S.A., Young L., Cote R.J., and Taylor C.R. Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J. Histochem. Cytochem. 43 (1995) 193
215. Shi S.R., Cote R.J., Young L., Imam S.A., and Taylor C.R. Use of pH 9.5 Tris-HCl buffer containing 5% urea for antigen retrieval immunohistochemistry. Biotech. Histochem. 71 (1996) 190
216. Shi S.R., Cote R.J., Yang C., Chen C., Xu H.J., Benedict W.F., and Taylor C.R. Development of an optimal protocol for antigen retrieval: a "test battery" approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J. Pathol. 179 (1996) 347
217. Shi S.R., Cote R.J., and Taylor C.R. Antigen retrieval immunohistochemistry: past, present, and future. J. Histochem. Cytochem. 45 (1997) 327
218. Shi S.R., Cote R.J., and Taylor C.R. Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: standardization and development. Auris, Nasus, Larynx 25 (1998) 425
219. Shi S.R., Cote R.J., Chaiwun B., Young L.L., Shi Y., Hawes D., Chen T., and Taylor C.R. Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 6 (1998) 89
220. Shi S.R., Cote R.J., Hawes D., Thu S., Shi Y., Young L.L., and Taylor C.R. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: what is the signal?. J. Histochem. Cytochem. 47 (1999) 463
221. Shi S.R., Gu J., Turrens J., Cote R.J., and Taylor C.R. Development of the antigen retrieval technique: philosophical and theoretical bases. In: Shi S.R., Gu J., and Taylor C.R. (Eds). Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology (2000), Eaton Publishing, Natick 17
222. Shi S.R., Cote R.J., and Taylor C.R. Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem. 49 (2001) 931
223. Shi S.R., Cote R.J., Liu C., Yu M.C., Castelao J.E., Ross R.K., and Taylor C.R. A modified reduced-temperature antigen retrieval protocol effective for use with a polyclonal antibody to cyclooxygenase-2 (PG 27). Appl. Immunohistochem. Mol. Morphol. 10 (2002) 368
224. Shi S.R., Liu C., Pootrakul L., Tang L., Young A., Chen R., Cote R.J., and Taylor C.R. Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry. Am. J. Clin. Pathol. 129 (2008) 358
225. Shidham V.B., Chang C.C., Rao R.N., Komorowski R., and Chivukula M. Immunostaining of cytology smears: a comparative study to identify the most suitable method of smear preparation and fixation with reference to commonly used immunomarkers. Diagn. Cytopathol. 29 (2003) 217
226. Shin R.W., Iwaki T., and Tateishi J. Hydrated autoclave pretreatment enhances Tau immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues. Lab. Invest. 64 (1991) 693
227. Shin H.J., Shin D.M., Shah T., and Ro J.Y. Methods in pathology. Optimization of proliferating cell nuclear antigen immunohistochemical staining by microwave heating in zinc sulfate solution. Mod. Pathol. 7 (1994) 242
228. Shintaku I.P., and Said J.W. Detection of estrogen receptors with monoclonal antibodies in routinely processed formalin-fixed paraffin sections of breast carcinoma. Use of DNase pretreatment to enhance sensitività of the reaction. Am. J. Clin. Pathol. 87 (1987) 161
229. Shiurba R.A., Ishiguro K., Takahashi M., Sato K., Spooner E.T., Mercken M., Yoshida R., Wheelock T.R., Yanagawa H., Imahori K., and Nixon R.A. Immunocytochemistry of tau phosphoserine 413 and tau protein kinase I in Alzheimer pathology. Brain Res. 737 (1996) 119
230. Shiurba R.A., Spooner E.T., Ishiguro K., Takahashi M., Yoshida R., Wheelock T.R., Imahori K., Cataldo A.M., and Nixon R.A. Immunocytochemistry of formalin-fixed human brain tissues: microwave irradiation of free-floating sections. Brain Res. Protoc. 2 (1998) 109
231. Silva M.T. Electron microscopic study on the effect of the oxidation of ultrathin sections of Bacillus cereus and Bacillus megaterium. J. Ultrastruct. Res. 18 (1967) 345
232. Silver M.M., Lines L.D., and Hearn S.S. Immunogold detection of chromogranin A in the neuroendocrine tumor. Ultrastruct. Pathol. 17 (1993) 83
233. Solberg L.B., Melhus G., Brorson S.H., Wende M., and Reinholt F.P. Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone. Micron 37 (2006) 347
234. Soltys B.J., and Gupta R.S. Interrelationships of endoplasmic reticulum, mitochondria, intermediate filaments, and microtubules - a quadruple fluorescence labeling study. Biochem. Cell. Biol. 70 (1992) 1174
235. Sompuram S.R., Vani K., Messana E., and Bogen S.A. A molecular mechanism of formalin fixation and antigen retrieval. Am. J. Clin. Pathol. 121 (2004) 190
236. Sompuram S.R., Vani K., and Bogen S.A. A molecular model of antigen retrieval using a peptide array. Am. J. Clin. Pathol. 125 (2006) 91
237. Sompuram S.R., Vani K., Hafer L.J., and Bogen S.A. Antibodies immunoreactive with formalin-fixed tissue antigens recognize linear protein epitopes. Am. J. Clin. Pathol. 125 (2006) 82
238. Sormunen R., and Leong A.S.Y. Microwave-stimulated antigen retrieval for immunohistology and immunoelectron microscopy of resin-embedded sections. Appl. Immunohistochem. 6 (1998) 234
239. Stacey K.A., Cobb M., Cousens S.F., and Alexander P. The reactions of the radiomimetic alkylating agents with the macromolecules in vitro. Ann. N.Y. Acad. Sci. 68 (1958) 682
240. Stein H., Garter K., Asbahr H., and Mason D.Y. Use of freeze-dried paraffin-embedded sections for immunohistologic staining with monoclonal antibodies. Lab. Invest. 52 (1985) 676
241. Stirling J.W., and Graff P.S. Antigen unmasking for immunoelectron microscopy: labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium. J. Histochem. Cytochem. 43 (1995) 115
242. Stone J.R., Walker S.A., and Povlishock J.T. The visualization of a new class of traumatically injured axons through the use of a modified method of microwave antigen retrieval. Acta Neuropathol. 97 (1999) 335
243. Suurmeijer A.J.H. Microwave-stimulated antigen retried. A new method facilitating immunohistochemistry of formalin-fmed, paraffin embedded tissue. Histochem. J. 24 (1992) 597
244. Suurmeijer A.J.H., and Boon M.E. Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections. Eur. J. Morphol. 31 (1993) 144
245. Suurmeijer A.J.H., and Boon M.E. Optimizing keratin and vimentin retrieval in formalin-fixed. paraffin-embedded tissue with the use of heat and metal salts. Appl. Immunohistochem. 1 (1993) 143
246. Takada Y., Ohhashi T., Suzuki M., Kitahara K., and Kobayashi M. Immunohistochemical detection of retinal cones in monkey retina: light and electron microscopic study. Jpn. J. Ophthalmol. 44 (2000) 503
247. Taylor C.R. Paraffin section immunocytochemistry for estrogen receptor. The time has come. Cancer 77 (1996) 2419
248. Taylor C.R. Standardization in immunohistochemistry: the role of antigen retrieval in molecular morphology. Biotech. Histochem. 81 (2006) 3
249. Taylor C.R., Shi S.R., Chen C., Young L., Yang C., and Cote R.J. Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer. Biotech. Histochem. 71 (1996) 263
250. Taylor C.R., Shi S.R., and Cote R.J. Antigen retrieval for immunohistochemistry. Status and need for greater standardization. Appl. Immunohistochem. 4 (1996) 144
251. van den Berg F.M., Baas I.O., Polak M.M., and Offerhaus G.J.A. Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. Am. J. Pathol. 142 (1993) 381
252. van Hecke D. Routine immunohistochemical staining today: choices to make, challenges to take. J. Histotechnol. 25 (2002) 45
253. Vani K., Bogen S.A., and Sompuram S.R. A high throughput combinatorial library technique for identifying formalin-sensitive epitopes. J. Immunol. Methods 317 (2006) 80
254. Vidal S., Lombardero M., Sanchez P., Roman A., and Moya L. An easy method for removal of Epon resin from semi-thin sections. Application of the avidin-biotin technique. Histochem. J. 27 (1995) 204
255. Volkin D.B., and Klibanov A.M. Minimizing protein inactivation. In: Creighton T.E. (Ed). Protein Function. A Practical Approach (1989), IRL Press, Oxford 1
256. von Wasielewski R., Werner M., Nolte M., Wilkens L., and Georgii A. Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies. Histochemistry 102 (1994) 165
257. Wakamatsu K., Ghazizadeh M., Ishizaki M., Fukuda Y., and Yamanaka N. Optimizing collagen antigen unmasking in paraffin-embedded tissues. Histochem. J. 29 (1997) 65
258. Ward J.M., Erexson C.R., Faucette L.J., Foley J.F., Dijkstra C., and Cattoretti G. Immunohistochemical markers for the rodent immune system. Toxicol. Pathol. 34 (2006) 616
259. Werner M., Wasielewski R., and von Komminoth P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem. Cell Biol. 105 (1996) 253
260. Werner M., Chott A., Fabiano A., and Battifora H. Effect of formalin tissue fixation and processing on immunohistochemistry. Am. J. Surg. Pathol. 24 (2000) 1016
261. Wiley C.A., Burrola P.G., Buchmeier M.J., Wooddell M.K., Barry R.A., Prusiner S.B., and Lampert P.W. Immuno-gold localization of prion filaments in scrapie-infected hamster brains. Lab. Invest. 57 (1987) 646
262. Wilson E., Jackson S., Cruwys S., and Kerry P. An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J. Immunol. Methods 322 (2007) 137
263. Wong S.C.C., Chan J.K.C., Lo E.S.F., Chan A.K.C., Wong M.C.K., Chan C.M.L., Lam M.Y.Y., and Chan A.T.C. The contribution of bifunctional SkipDewax pretreatment solution, rabbit monoclonal antibodies, and polymer detection systems in immunohistochemistry. Arch. Pathol. Lab. Med. 131 (2007) 1047
264. Wood G.S., and Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J. Histochem. Cytochem. 29 (1981) 1196
265. Xiao J.-C., Adam A., Ruck P., and Kaiserling E. A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstration of cytokeratin no. 18 in normal and neoplastic hepatocytes. Biotech. Histochem. 71 (1996) 278
266. Yamashita S. Heat-induced antigen retrieval: mechanisms and application to histochemistry. Prog. Histochem. Cytochem. 41 (2007) 141
267. Yamashita S., and Okada Y. Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry. J. Histochem. Cytochem. 53 (2005) 13
268. Yamashita S., and Okada Y. Application of heat-induced antigen retrieval to aldehyde-fixed fresh frozen sections. J. Histochem. Cytochem. 53 (2005) 1421
269. Yamashita S., Aiso S., Shiozawa M., and Yasuda K. Immunohistochemical study of gamma-glutamyl transpeptidase with monoclonal antibodies. II. An immunoelectron microscopic study in rat kidney. Acta Histochem. Cytochem. 22 (1989) 367
270. Yamashita S., Sogo T., Shiozawa M., and Yasuda K. Immunolocalization of aldolase A subunit using monoclonal antibody in rabbit tissues. Acta Histochem. Cytochem. 30 (1997) 601
271. Yano S., Kashima K., Daa T., Urabe S., Tsuji K., Nakayama I., and Yokoyama S. An antigen retrieval method using an alkaline solution allows immunoelectron microscopic identification of secretory granule. J. Histochem. Cytochem. 51 (2003) 199
272. Yasuda K., Yamashita S., Aiso S., Shiozawa M., and Komatsu T. Immunohistochemical study of gamma-glutamyl transpeptidase with monoclonal antibodies. I. Preparation and characteristics of monoclonal antibodies to gamma-glutamyl transpeptidase. Acta Histochem. Cytochem. 19 (1986) 589
273. Zhai X.Y., Kristoffersen I.B., and Christensen E.I. Immunocytochemistry of renal membrane proteins on epoxy sections. Kidney Int. 72 (2007) 731