Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Huang, Ling ^a,b   Pike, Douglas ^a,b   Sleat, David E ^a,b   Nanda, Vikas ^a,b   Lobel, Peter ^a,b  

^a RUTGERS UNIVERSITY   (United States)

^b RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; MCHERRY FUSION PROTEIN; MEMBRANE PROTEIN; NIEMANN PICK DISEASE TYPE C PROTEIN 2; PROTEINASE; TRIPEPTIDYL PEPTIDASE I; UNCLASSIFIED DRUG; AMINOPEPTIDASE; DIPEPTIDYL PEPTIDASE; HYBRID PROTEIN; NPC2 PROTEIN, MOUSE; PHOTOPROTEIN; PRIMER DNA; RED FLUORESCENT PROTEIN; SERINE PROTEINASE; TRIPEPTIDYL-PEPTIDASE 1; VESICULAR TRANSPORT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LABELING; CELL ORGANELLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EMBRYO; FEMALE; GENE DELETION; LYSOSOME; MOLECULAR MODEL; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN SECRETION; PROTEIN TARGETING; AFFINITY CHROMATOGRAPHY; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CONFOCAL MICROSCOPY; DIAGNOSTIC USE; DRUG EFFECTS; GENETICS; GLYCOSYLATION; IMMUNOBLOTTING; METABOLISM; MOLECULAR CLONING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN TRANSPORT; TISSUE CULTURE TECHNIQUE; ULTRASTRUCTURE;

AMINOPEPTIDASES; ANIMALS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DIPEPTIDYL-PEPTIDASES AND TRIPEPTIDYL-PEPTIDASES; DNA PRIMERS; FLUORESCENT DYES; GLYCOSYLATION; IMMUNOBLOTTING; LUMINESCENT PROTEINS; LYSOSOMES; MICE; MICROSCOPY, CONFOCAL; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SERINE PROTEASES; TISSUE CULTURE TECHNIQUES; VESICULAR TRANSPORT PROTEINS;

EID: 84896791418     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0088893     Document Type: Article

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