Epitopes of microbial and human heat shock protein 60 and their recognition in myalgic encephalomyelitis

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Elfaitouri, Amal ^a   Herrmann, Björn ^a   Bölin Wiener, Agnes ^a   Wang, Yilin ^a   Gottfries, Carl Gerhard ^b   Zachrisson, Olof ^b   Pipkorn, Rüdiger ^c   Rönnblom, Lars ^a   Blomberg, Jonas ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b Gottfries Clinic AB   (Sweden)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONIN 60; EPITOPE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; RECOMBINANT PROTEIN; AUTOANTIBODY; BACTERIAL PROTEIN;

ANTIGENICITY; ARTICLE; CHLAMYDIA; CHLAMYDOPHILA PNEUMONIAE; CHRONIC FATIGUE SYNDROME; CONTROLLED STUDY; EPITOPE MAPPING; HUMAN; HUMORAL IMMUNITY; MAJOR CLINICAL STUDY; MULTIPLE SCLEROSIS; MYCOPLASMA; NONHUMAN; PLASMODIUM; STAPHYLOCOCCUS; SYSTEMIC LUPUS ERYTHEMATOSUS; IMMUNOLOGY;

AUTOANTIBODIES; BACTERIAL PROTEINS; CHAPERONIN 60; EPITOPES; FATIGUE SYNDROME, CHRONIC; HUMANS;

EID: 84896728657     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081155     Document Type: Article

References (93)

1. Bukau B, Horwich AL (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92: 351-366. doi:10.1016/S0092-8674(00)80928-9. PubMed: 9476895. (Pubitemid 28093013)
2. Zügel U, Kaufmann SH (1999) Role of heat shock proteins in protection from and pathogenesis of infectious diseases. Clin Microbiol Rev 12: 19-39. PubMed: 9880473. (Pubitemid 29055952)
3. Van Noort JM, van Sechel AC, van Stipdonk MJ, Bajramovic JJ (1998) The small heat shock protein alpha B-crystallin as key autoantigen in multiple sclerosis. Prog Brain Res 117: 435-452. doi:10.1016/S0079-6123(08)64031-5. PubMed: 9932424. (Pubitemid 28530705)
4. Quintana FJ, Farez MF, Viglietta V, Iglesias AH, Merbl Y et al. (2008) Antigen microarrays identify unique serum autoantibody signatures in clinical and pathologic subtypes of multiple sclerosis. Proc Natl Acad Sci U S A 105: 18889-18894. doi:10.1073/pnas.0806310105. PubMed: 19028871.
5. Mansilla MJ, Montalban X, Espejo C (2012) Heat shock protein 70: roles in multiple sclerosis. Mol Med 18: 1018-1028. PubMed: 22669475.
6. Horváth L, Cervenak L, Oroszlán M, Prohászka Z, Uray K et al. (2002) Antibodies against different epitopes of heat-shock protein 60 in children with type 1 diabetes mellitus. Immunol Lett 80: 155-162. doi:10.1016/S0165-2478(01)00336-4. PubMed: 11803047. (Pubitemid 34136002)
7. Jones DB, Coulson AF, Duff GW (1993) Sequence homologies between hsp60 and autoantigens. Immunol Today 14: 115-118. doi: 10.1016/0167-5699(93)90210-C. PubMed: 8466626. (Pubitemid 23068294)
8. Lorusso L, Mikhaylova SV, Capelli E, Ferrari D, Ngonga GK et al. (2009) Immunological aspects of chronic fatigue syndrome. Autoimmun Rev 8: 287-291. doi:10.1016/j.autrev.2008.08.003. PubMed: 18801465.
9. Konstantinov K, von Mikecz A, Buchwald D, Jones J, Gerace L et al. (1996) Autoantibodies to nuclear envelope antigens in chronic fatigue syndrome. J Clin Invest 98: 1888-1896. doi:10.1172/JCI118990. PubMed: 8878441. (Pubitemid 26359207)
10. Ortega-Hernandez OD, Shoenfeld Y (2009) Infection, vaccination, and autoantibodies in chronic fatigue syndrome, cause or coincidence? Ann N Y Acad Sci 1173: 600-609. doi:10.1111/j.1749-6632.2009.04799.x. PubMed: 19758205.
11. Ortega-Hernandez OD, Cuccia M, Bozzini S, Bassi N, Moscavitch S et al. (2009) Autoantibodies, polymorphisms in the serotonin pathway, and human leukocyte antigen class II alleles in chronic fatigue syndrome: are they associated with age at onset and specific symptoms? Ann N Y Acad Sci 1173: 589-599. doi:10.1111/j.1749-6632.2009.04802.x. PubMed: 19758204.
12. Hokama Y, Campora CE, Hara C, Kuribayashi T, Le Huynh D et al. (2009) Anticardiolipin antibodies in the sera of patients with diagnosed chronic fatigue syndrome. J Clin Lab Anal 23: 210-212. doi:10.1002/jcla.20325. PubMed: 19623655.
13. Maes M, Mihaylova I, Kubera M, Leunis JC, Twisk FN et al. (2012) IgM-mediated autoimmune responses directed against anchorage epitopes are greater in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) than in major depression. Metab Brain Dis 27: 415-423. doi:10.1007/s11011-012-9316-8. PubMed: 22614823.
14. Maes M, Mihaylova I, Leunis JC (2007) Increased serum IgA and IgM against LPS of enterobacteria in chronic fatigue syndrome (CFS): indication for the involvement of gram-negative enterobacteria in the etiology of CFS and for the presence of an increased gut-intestinal permeability. J Affect Disord 99: 237-240. doi:10.1016/j.jad.2006.08.021. PubMed: 17007934.
15. Booth NE, Myhill S, McLaren-Howard J (2012) Mitochondrial dysfunction and the pathophysiology of Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome (ME/CFS). Int J Clin Exp Med 5: 208-220. PubMed: 22837795.
16. Ablashi DV, Zompetta C, Lease C, Josephs SF, Balachandra N, et al. (1991) Human herpesvirus 6 (HHV6) and chronic fatigue syndrome (CFS). Canada diseases weekly report = Rapport hebdomadaire des maladies au Canada 17 Suppl 1E: 33-40
17. Cameron B, Flamand L, Juwana H, Middeldorp J, Naing Z et al. (2010) Serological and virological investigation of the role of the herpesviruses EBV, CMV and HHV-6 in post-infective fatigue syndrome. J Med Virol 82: 1684-1688. doi:10.1002/jmv.21873. PubMed: 20827765.
18. Kozireva S, Nemceva G, Danilane I, Pavlova O, Blomberg J et al. (2001) Prevalence of blood-borne viral infections (cytomegalovirus, human herpesvirus-6, human herpesvirus-7, human herpesvirus-8, human T-cell lymphotropic virus-I/II, human retrovirus-5) among blood donors in Latvia. Ann Hematol 80: 669-673. doi:10.1007/s002770100359. PubMed: 11757726. (Pubitemid 33725592)
19. Sairenji T, Yamanishi K, Tachibana Y, Bertoni G, Kurata T (1995) Antibody responses to Epstein-Barr virus, human herpesvirus 6 and human herpesvirus 7 in patients with chronic fatigue syndrome. Intervirology 38: 269-273. doi:10.1159/000150450. PubMed: 8724857. (Pubitemid 26063983)
20. Kawai K, Kawai A (1992) Studies on the relationship between chronic fatigue syndrome and Epstein-Barr virus in Japan. Intern Med 31: 313-318. doi:10.2169/internalmedicine.31.313. PubMed: 1319246.
21. Lerner AM, Ariza ME, Williams M, Jason L, Beqaj S et al. (2012) Antibody to Epstein-Barr virus deoxyuridine triphosphate nucleotidohydrolase and deoxyribonucleotide polymerase in a chronic fatigue syndrome subset. PLOS ONE 7: e47891. doi:10.1371/journal.pone.0047891. PubMed: 23155374.
22. Lerner AM, Beqaj SH, Deeter RG, Dworkin HJ, Zervos M et al. (2002) A six-month trial of valacyclovir in the Epstein-Barr virus subset of chronic fatigue syndrome: improvement in left ventricular function. Drugs Today (Barc) 38: 549-561. doi:10.1358/dot.2002.38.8.820095. PubMed: 12582420.
23. Lerner AM, Beqaj SH, Deeter RG, Fitzgerald JT (2004) IgM serum antibodies to Epstein-Barr virus are uniquely present in a subset of patients with the chronic fatigue syndrome. In Vivo 18: 101-106. PubMed: 15113035. (Pubitemid 38488991)
24. Lerner AM, Beqaj SH, Deeter RG, Fitzgerald JT (2007) Valacyclovir treatment in Epstein-Barr virus subset chronic fatigue syndrome: thirty-six months follow-up. In Vivo 21: 707-713. PubMed: 18019402.
25. Natelson BH, Ye N, Moul DE, Jenkins FJ, Oren DA et al. (1994) High titers of anti-Epstein-Barr virus DNA polymerase are found in patients with severe fatiguing illness. J Med Virol 42: 42-46. doi:10.1002/jmv.1890420109. PubMed: 8308519. (Pubitemid 24043052)
26. Petrov D, Marchalik D, Sosin M, Bal A (2012) Factors affecting duration of chronic fatigue syndrome in pediatric patients. Indian J Pediatr 79: 52-55. doi:10.1007/s12098-011-0463-4. PubMed: 21617905.
27. Sumaya CV (1991) Serologic and virologic epidemiology of Epstein-Barr virus: relevance to chronic fatigue syndrome. Rev Infect Dis 13 Suppl 1: S19-S25. doi:10.1093/clinids/12.5.19. PubMed: 1850540.
28. Swanink CM, van der Meer JW, Vercoulen JH, Bleijenberg G, Fennis JF et al. (1995) Epstein-Barr virus (EBV) and the chronic fatigue syndrome: normal virus load in blood and normal immunologic reactivity in the EBV regression assay. Clin Infect Dis 20: 1390-1392. doi:10.1093/clinids/20.5.1390. PubMed: 7620030.
29. Whelton CL, Salit I, Moldofsky H (1992) Sleep, Epstein-Barr virus infection, musculoskeletal pain, and depressive symptoms in chronic fatigue syndrome. J Rheumatol 19: 939-943. PubMed: 1328633.
30. Chia J, Chia A, Voeller M, Lee T, Chang R (2010) Acute enterovirus infection followed by myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and viral persistence. J Clin Pathol 63: 165-168. doi:10.1136/jcp.2009. 070466. PubMed: 19828908.
31. Kerr JR (2008) Enterovirus infection of the stomach in chronic fatigue syndrome/myalgic encephalomyelitis. J Clin Pathol 61: 1-2. PubMed: 17873115.
32. Zhang L, Gough J, Christmas D, Mattey DL, Richards SC et al. (2010) Microbial infections in eight genomic subtypes of chronic fatigue syndrome/myalgic encephalomyelitis. J Clin Pathol 63: 156-164. doi:10.1136/jcp.2009.072561. PubMed: 19955554.
33. Kerr JR, Gough J, Richards SC, Main J, Enlander D et al. (2010) Antibody to parvovirus B19 nonstructural protein is associated with chronic arthralgia in patients with chronic fatigue syndrome/myalgic encephalomyelitis. J Gen Virol 91: 893-897. doi:10.1099/vir.0.017590-0. PubMed: 20007355.
34. Chia JK, Chia LY (1999) Chronic Chlamydia pneumoniae infection: a treatable cause of chronic fatigue syndrome. Clin Infect Dis 29: 452-453. doi:10.1086/520239. PubMed: 10476765. (Pubitemid 29380223)
35. Komaroff AL, Wang SP, Lee J, Grayston JT (1992) No association of chronic Chlamydia pneumoniae infection with chronic fatigue syndrome. J Infect Dis 165: 184. doi:10.1093/infdis/165.1.184. PubMed: 1727893.
36. Endresen GK (2003) Mycoplasma blood infection in chronic fatigue and fibromyalgia syndromes. Rheumatol Int 23: 211-215. doi:10.1007/s00296-003-0355- 7. PubMed: 12879275.
37. Komaroff AL, Bell DS, Cheney PR, Lo SC (1993) Absence of antibody to Mycoplasma fermentans in patients with chronic fatigue syndrome. Clin Infect Dis 17: 1074-1075. doi:10.1093/clinids/17.6.1074. PubMed: 8110942. (Pubitemid 23358767)
38. Nasralla M, Haier J, Nicolson GL (1999) Multiple mycoplasmal infections detected in blood of patients with chronic fatigue syndrome and/or fibromyalgia syndrome. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 18: 859-865
39. Nijs J, Nicolson GL, De Becker P, Coomans D, De Meirleir K (2002) High prevalence of Mycoplasma infections among European chronic fatigue syndrome patients. Examination of four Mycoplasma species in blood of chronic fatigue syndrome patients. FEMS Immunol Med Microbiol 34: 209-214. doi:10.1111/j.1574- 695X.2002.tb00626.x. PubMed: 12423773. (Pubitemid 35335521)
40. Vojdani A, Choppa PC, Tagle C, Andrin R, Samimi B et al. (1998) Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome. FEMS Immunol Med Microbiol 22: 355-365. doi:10.1111/j.1574- 695X.1998.tb01226.x. PubMed: 9879928. (Pubitemid 29010294)
41. Blomberg J, Sheikholvaezin A, Elfaitouri A, Blomberg F, Sjosten A, et al. (2011) Phylogeny-directed search for murine leukemia virus-like retroviruses in vertebrate genomes and in patients suffering from myalgic encephalomyelitis/ chronic fatigue syndrome and prostate cancer. Advances in Virology 2011: 341294
42. Blomberg J, Blomberg F, Sjösten A, Sheikholvaezin A, Bölin-Wiener A et al. (2012) No evidence for xenotropic murine leukemia-related virus infection in Sweden using internally controlled multiepitope suspension array serology. Clin Vaccine Immunol 19: 1399-1410. doi:10.1128/CVI.00391-12. PubMed: 22787191.
43. Thambirajah AA, Sleigh K, Stiver HG, Chow AW (2008) Differential heat shock protein responses to strenuous standardized exercise in chronic fatigue syndrome patients and matched healthy controls. Clin Invest Med 31: E319-E327. PubMed: 19032901.
44. Braig K, Otwinowski Z, Hegde R, Boisvert DC, Joachimiak A et al. (1994) The crystal structure of the bacterial chaperonin GroEL at 2.8 A. Nature 371: 578-586. doi:10.1038/371578a0. PubMed: 7935790.
45. Chen L, Sigler PB (1999) The crystal structure of a GroEL/peptide complex: plasticity as a basis for substrate diversity. Cell 99: 757-768. doi:10.1016/S0092-8674(00)81673-6. PubMed: 10619429. (Pubitemid 30017646)
46. Michaëlsson J, Teixeira de Matos C, Achour A, Lanier LL, Kärre K et al. (2002) A signal peptide derived from hsp60 binds HLA-E and interferes with CD94/NKG2A recognition. J Exp Med 196: 1403-1414. doi:10.1084/jem.20020797. PubMed: 12461076.
47. Dyall SD, Brown MT, Johnson PJ (2004) Ancient invasions: from endosymbionts to organelles. Science 304: 253-257. doi:10.1126/science.1094884. PubMed: 15073369. (Pubitemid 38452171)
48. Cappello F, Conway de Macario E, Di Felice V, Zummo G, Macario AJ (2009) Chlamydia trachomatis infection and anti-Hsp60 immunity: the two sides of the coin. PLoS Pathog 5: e1000552. PubMed: 19714222.
49. Hansen JJ, Dürr A, Cournu-Rebeix I, Georgopoulos C, Ang D et al. (2002) Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet 70: 1328-1332. doi:10.1086/339935. PubMed: 11898127. (Pubitemid 34450582)
50. Ranford JC, Henderson B (2002) Chaperonins in disease: mechanisms, models, and treatments. Mol Pathol 55: 209-213. doi:10.1136/mp.55.4.209. PubMed: 12147708.
51. Durai M, Huang MN, Moudgil KD (2009) Self heat-shock protein 65-mediated regulation of autoimmune arthritis. J Autoimmun 33: 208-213. doi:10.1016/j.jaut.2009.09.007. PubMed: 19800761.
52. Kim HR, Kim EY, Cerny J, Moudgil KD (2006) Antibody responses to mycobacterial and self heat shock protein 65 in autoimmune arthritis: epitope specificity and implication in pathogenesis. J Immunol 177: 6634-6641. PubMed: 17082575. (Pubitemid 44715026)
53. van Noort JM, Bajramovic JJ, Plomp AC, van Stipdonk MJ (2000) Mistaken self, a novel model that links microbial infections with myelin-directed autoimmunity in multiple sclerosis. J Neuroimmunol 105: 46-57. doi:10.1016/S0165-5728(00)00181-8. PubMed: 10713363. (Pubitemid 30125428)
54. Salinas GF, Braza F, Brouard S, Tak PP, Baeten D (2013) The role of B lymphocytes in the progression from autoimmunity to autoimmune disease. Clin Immunol 146: 34-45. doi:10.1016/j.clim.2012.10.005. PubMed: 23202542.
55. Deshmukh US, Gaskin F, Lewis JE, Kannapell CC, Fu SM (2003) Mechanisms of autoantibody diversification to SLE-related autoantigens. Ann N Y Acad Sci 987: 91-98. doi:10.1111/j.1749-6632.2003.tb06036.x. PubMed: 12727627. (Pubitemid 36584010)
56. Xue Y, O'Mara ML, Surawski PP, Trau M, Mark AE (2011) Effect of poly(ethylene glycol) (PEG) spacers on the conformational properties of small peptides: a molecular dynamics study. Langmuir 27: 296-303. doi:10.1021/ la103800h. PubMed: 21121595.
57. Maeda H, Miyamoto M, Hongyo H, Nagai A, Kurihara H et al. (1994) Heat shock protein 60 (GroEL) from Porphyromonas gingivalis: molecular cloning and sequence analysis of its gene and purification of the recombinant protein. FEMS Microbiol Lett 119: 129-135. doi:10.1111/j.1574-6968.1994.tb06879.x. PubMed: 7913687. (Pubitemid 124012166)
58. Maeda H, Miyamoto M, Kokeguchi S, Kono T, Nishimura F et al. (2000) Epitope mapping of heat shock protein 60 (GroEL) from Porphyromonas gingivalis. FEMS Immunol Med Microbiol 28: 219-224. doi:10.1111/j.1574-695X.2000.tb01480.x. PubMed: 10865174. (Pubitemid 32939404)
59. Uray K, Hudecz F, Füst G, Prohászka Z (2003) Comparative analysis of linear antibody epitopes on human and mycobacterial 60-kDa heat shock proteins using samples of healthy blood donors. Int Immunol 15: 1229-1236. doi:10.1093/intimm/dxg122. PubMed: 13679392. (Pubitemid 37220496)
60. Perschinka H, Mayr M, Millonig G, Mayerl C, van der Zee R et al. (2003) Cross-reactive B-cell epitopes of microbial and human heat shock protein 60/65 in atherosclerosis. Arterioscler Thromb Vasc Biol 23: 1060-1065. doi:10.1161/01.ATV.0000071701.62486.49. PubMed: 12702515. (Pubitemid 36713561)
61. Perschinka H, Wellenzohn B, Parson W, van der Zee R, Willeit J et al. (2007) Identification of atherosclerosis-associated conformational heat shock protein 60 epitopes by phage display and structural alignment. Atherosclerosis 194: 79-87. doi:10.1016/j.atherosclerosis.2006.09.028. PubMed: 17097662. (Pubitemid 47464310)
62. Wysocki J, Karawajczyk B, Górski J, Korzeniowski A, Maćkiewicz Z et al. (2002) Human heat shock protein 60 (409-424) fragment is recognized by serum antibodies of patients with acute coronary syndromes. Cardiovasc Pathol 11: 238-243. doi:10.1016/S1054-8807(02)00109-6. PubMed: 12140130. (Pubitemid 34828276)
63. Härkönen T, Puolakkainen M, Sarvas M, Airaksinen U, Hovi T et al. (2000) Picornavirus proteins share antigenic determinants with heat shock proteins 60/65. J Med Virol 62: 383-391. doi:10.1002/1096-9071(200011)62:3. PubMed: 11055249.
64. Metzler B, Schett G, Kleindienst R, van der Zee R, Ottenhoff T et al. (1997) Epitope specificity of anti-heat shock protein 65/60 serum antibodies in atherosclerosis. Arterioscler Thromb Vasc Biol 17: 536-541. doi:10.1161/01.ATV. 17.3.536. PubMed: 9102173. (Pubitemid 27133144)
65. Elias D, Cohen IR (1994) Peptide therapy for diabetes in NOD mice. Lancet 343: 704-706. doi:10.1016/S0140-6736(94)91582-2. PubMed: 7907681. (Pubitemid 24087851)
66. Elias D, Cohen IR (1995) Treatment of autoimmune diabetes and insulitis in NOD mice with heat shock protein 60 peptide p277. Diabetes 44: 1132-1138. doi:10.2337/diab.44.9.1132. PubMed: 7657040.
67. Elias D, Cohen IR (1996) The hsp60 peptide p277 arrests the autoimmune diabetes induced by the toxin streptozotocin. Diabetes 45: 1168-1172. doi:10.2337/diabetes.45.9.1168. PubMed: 8772717. (Pubitemid 26296314)
68. Elias D, Marcus H, Reshef T, Ablamunits V, Cohen IR (1995) Induction of diabetes in standard mice by immunization with the p277 peptide of a 60-kDa heat shock protein. Eur J Immunol 25: 2851-2857. doi:10.1002/eji.1830251021. PubMed: 7589082.
69. Elias D, Meilin A, Ablamunits V, Birk OS, Carmi P et al. (1997) Hsp60 peptide therapy of NOD mouse diabetes induces a Th2 cytokine burst and downregulates autoimmunity to various beta-cell antigens. Diabetes 46: 758-764. doi:10.2337/diab.46.5.758. PubMed: 9133541. (Pubitemid 27188251)
70. Elias D, Prigozin H, Polak N, Rapoport M, Lohse AW et al. (1994) Autoimmune diabetes induced by the beta-cell toxin STZ. Immunity to the 60-kDa heat shock protein and to insulin. Diabetes 43: 992-998. doi:10.2337/diab.43.8. 992. PubMed: 8039607.
71. Elias D, Tikochinski Y, Frankel G, Cohen IR (1999) Regulation of NOD mouse autoimmune diabetes by T cells that recognize a TCR CDR3 peptide. Int Immunol 11: 957-966. doi:10.1093/intimm/11.6.957. PubMed: 10360970. (Pubitemid 29276608)
72. Sziller I, Witkin SS, Ziegert M, Csapó Z, Ujházy A et al. (1998) Serological responses of patients with ectopic pregnancy to epitopes of the Chlamydia trachomatis 60 kDa heat shock protein. Human Reproduction 13: 1088-1093. doi:10.1093/humrep/13.4.1088. PubMed: 9619577. (Pubitemid 28239227)
73. Domeika M, Domeika K, Paavonen J, Mårdh PA, Witkin SS (1998) Humoral immune response to conserved epitopes of Chlamydia trachomatis and human 60-kDa heat-shock protein in women with pelvic inflammatory disease. J Infect Dis 177: 714-719. doi:10.1086/514218. PubMed: 9498452. (Pubitemid 28099887)
74. Thomas S, Thirumalapura NR, Crocquet-Valdes PA, Luxon BA, Walker DH (2011) Structure-based vaccines provide protection in a mouse model of ehrlichiosis. PLOS ONE 6: e27981. doi:10.1371/journal.pone.0027981. PubMed: 22114733.
75. Lachumanan R, Devi S, Cheong YM, Rodda SJ, Pang T (1993) Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. Infect Immun 61: 4527-4531. PubMed: 7691753. (Pubitemid 23298625)
76. Lin J, Adams LG, Ficht TA (1996) Immunological response to the Brucella abortus GroEL homolog. Infect Immun 64: 4396-4400. PubMed: 8926120. (Pubitemid 26318174)
77. Yong L, Yunxiao S, Qiyan X, Yu Z, Jing H et al. (2010) Immunization with P277 induces vascular leak syndrome in C57BL/6 mice via endothelial damage. Autoimmunity 43: 654-663. doi:10.3109/08916931003674683. PubMed: 20429836.
78. Yokota SI, Hirata D, Minota S, Higashiyama T, Kurimoto M et al. (2000) Autoantibodies against chaperonin CCT in human sera with rheumatic autoimmune diseases: comparison with antibodies against other Hsp60 family proteins. Cell Stress Chaperones 5: 337-346. doi:10.1379/1466-1268(2000)005. PubMed: 11048656.
79. Vanness JM, Snell CR, Strayer DR, Dempsey. Lt, Stevens SR (2003) Subclassifying chronic fatigue syndrome through exercise testing. Medicine and science in sports and exercise 35: 908-913 (Pubitemid 36706277)
80. White AT, Light AR, Hughen RW, Bateman L, Martins TB et al. (2010) Severity of symptom flare after moderate exercise is linked to cytokine activity in chronic fatigue syndrome. Psychophysiology 47: 615-624. PubMed: 20230500.
81. Myhill S, Booth NE, McLaren-Howard J (2009) Chronic fatigue syndrome and mitochondrial dysfunction. Int J Clin Exp Med 2: 1-16. PubMed: 19436827.
82. Lodi R, Taylor DJ, Radda GK (1997) Chronic fatigue syndrome and skeletal muscle mitochondrial function. Muscle Nerve 20: 765-766. PubMed: 9149090. (Pubitemid 27197735)
83. Plioplys AV, Plioplys S (1995) Electron-microscopic investigation of muscle mitochondria in chronic fatigue syndrome. Neuropsychobiology 32: 175-181. doi:10.1159/000119233. PubMed: 8587699.
84. Ichiki Y, Leung PS, Ishibashi H, Coppel RL, Ansari AA et al. (2004) Mitochondria and autoimmunity in primary biliary cirrhosis. Mitochondrion 4: 743-753. doi:10.1016/j.mito.2004.07.024. PubMed: 16120429. (Pubitemid 39646819)
85. Brenu EW, van Driel ML, Staines DR, Ashton KJ, Hardcastle SL et al. (2012) Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis. J Transl Med 10: 88. doi:10.1186/1479-5876-10-88. PubMed: 22571715.
86. Fletcher MA, Zeng XR, Maher K, Levis S, Hurwitz B et al. (2010) Biomarkers in chronic fatigue syndrome: evaluation of natural killer cell function and dipeptidyl peptidase IV/CD26. PLOS ONE 5: e10817. doi:10.1371/journal.pone.0010817. PubMed: 20520837.
87. Fluge O, Bruland O, Risa K, Storstein A, Kristoffersen EK et al. (2011) Benefit from B-lymphocyte depletion using the anti-CD20 antibody rituximab in chronic fatigue syndrome. A double-blind and placebo-controlled study. PLOS ONE 6: e26358. doi:10.1371/journal.pone.0026358. PubMed: 22039471.
88. Fluge O, Mella O (2009) Clinical impact of B-cell depletion with the anti- CD20 antibody rituximab in chronic fatigue syndrome: a preliminary case series. BMC Neurol 9: 28. doi:10.1186/1471-2377-9-28. PubMed: 19566965.
89. Carruthers BM (2007) Definitions and aetiology of myalgic encephalomyelitis: how the Canadian consensus clinical definition of myalgic encephalomyelitis works. J Clin Pathol 60: 117-119. PubMed: 16935963.
90. Wolfe F, Smythe HA, Yunus MB, Bennett RM, Bombardier C et al. (1990) The American College of Rheumatology 1990 Criteria for the Classification of Fibromyalgia. Report of the Multicenter Criteria Committee. Arthritis Rheum 33: 160-172. doi:10.1002/art.1780330203. PubMed: 2306288. (Pubitemid 20098653)
91. Drossman DA (1999) The functional gastrointestinal disorders and the Rome II process. Gut 45 Suppl 2: II1-II5. doi:10.1136/gut.45.2008.ii1. PubMed: 10457038.
92. Johansson B, Carlsson A, Carlsson ML, Karlsson M, Nilsson MKL et al. (2012) Placebo-controlled cross-over study of the monoaminergic stabiliser (-)-OSU6162 in mental fatigue following stroke or traumatic brain injury. Acta Neuropsychiatrica 24: 266-274. doi:10.1111/j.1601-5215.2012.00678.x.
93. Blomberg J, Nilsson I, Andersson M (1983) Viral antibody screening system that uses a standardized single dilution immunoglobulin G enzyme immunoassay with multiple antigens. J Clin Microbiol 17: 1081-1091. PubMed: 6308038. (Pubitemid 13098872)