Processing of recombinant spider silk proteins into tailor-made materials for biomaterials applications

Current Opinion in Biotechnology

Volumn 29, Issue 1, 2014, Pages 62-69

  Schacht, Kristin ^a   Scheibel, Thomas ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCOMPATIBILITY; DRUG DELIVERY; MECHANICAL PROPERTIES; MEDICAL APPLICATIONS; RECOMBINANT PROTEINS;

BIOMATERIALS APPLICATIONS; BIOMEDICAL APPLICATIONS; DRUG DELIVERY SYSTEM; GENETIC MODIFICATIONS; NATURAL MATERIALS; RECOMBINANT SPIDER SILK PROTEINS; SCAFFOLDS FOR TISSUE ENGINEERING; SCALABLE PRODUCTION;

BIOLOGICAL MATERIALS;

BIOMATERIAL; CHIMERIC PROTEIN; INSECT PROTEIN; RECOMBINANT PROTEIN; SILK; SPIDER SILK PROTEIN; TISSUE SCAFFOLD; UNCLASSIFIED DRUG; DNA; NATURAL PRODUCT; POLYMER; ARTHROPOD PROTEIN;

AMINO ACID SEQUENCE; DNA MODIFICATION; DRUG DELIVERY SYSTEM; MATERIAL COATING; MICROCAPSULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN PROCESSING; PROTEIN SYNTHESIS; REVIEW; SPIDER; TISSUE ENGINEERING; WOUND DRESSING; BIOTECHNOLOGY; ENCAPSULATION; IMPLANT; TISSUE SCAFFOLD; ANIMAL; BIOSYNTHESIS; GENETICS; HUMAN; METABOLISM;

ANIMALS; ARTHROPOD PROTEINS; BIOCOMPATIBLE MATERIALS; BIOTECHNOLOGY; DRUG DELIVERY SYSTEMS; HUMANS; RECOMBINANT PROTEINS; SILK; SPIDERS;

EID: 84896541762     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2014.02.015     Document Type: Review

References (71)

1. Gerritsen V.B. The tiptoe of an airbus. Protein Spotlight Swiss Prot 2002, 24:1-2.
2. Gosline J.M., Guerette P.A., Ortlepp C.S., Savage K.N. The mechanical design of spider silks: from fibroin sequence to mechanical function. J Exp Biol 1999, 202:3295-3303.
3. Altman G.H., Diaz F., Jakuba C., Calabro T., Horan R.L., Chen J.S., Lu H., Richmond J., Kaplan D.L. Silk-based biomaterials. Biomaterials 2003, 24:401-416.
4. Vollrath F., Barth P., Basedow A., Engström W., List H. Local tolerance to spider silks and protein polymers in vivo. In Vivo 2002, 16:229-234.
5. Heidebrecht A., Scheibel T. Recombinant production of spider silk proteins. Adv Appl Microbiol 2013, 82:115-153.
6. Aprhisiart A., Vollrath F. Design-features of the orb web of the spider, Araneus-diadematus. Behav Ecol 1994, 5:280-287.
7. Hinman M.B., Lewis R.V. Isolation of a clone encoding a 2nd dragline silk fibroin - Nephila-clavipes dragline silk is a 2-protein fiber. J Biol Chem 1992, 267:19320-19324.
8. Ayoub N.A., Garb J.E., Tinghitella R.M., Collin M.A., Hayashi C.Y. Blueprint for a high-performance biomaterial: full-length spider dragline silk genes. PLoS ONE 2007, 2:e514.
9. Andersen S.O. Amino acid composition of spider silds. Compar Biochem Physiol 1970, 35:705-711.
10. Kummerlen J., van Beek J.D., Vollrath F., Meier B.H. Local structure in spider dragline silk investigated by two-dimensional spin-diffusion nuclear magnetic resonance. Macromolecules 1996, 29:2920-2928.
11. Hayashi C.Y., Shipley N.H., Lewis R.V. Hypotheses that correlate the sequence, structure, and mechanical properties of spider silk proteins. Int J Biol Macromol 1999, 24:271-275.
12. Askarieh G., Hedhammar M., Nordling K., Saenz A., Casals C., Rising A., Johansson J., Knight S.D. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature 2010, 465:236-239.
13. Eisoldt L., Smith A., Scheibel T. Decoding the secrets of spider silk. Mater Today 2011, 14:80-86.
14. Hagn F., Eisoldt L., Hardy J., Vendrely C., Coles M., Scheibel T., Kessler H. A highly conserved spider silk domain acts as a molecular switch that controls fibre assembly. Nature 2010, 465:239-242.
15. Hagn F., Thamm C., Scheibel T., Kessler H. pH-dependent dimerization and salt-dependent stabilization of the N-terminal domain of spider dragline silk-implications for fiber formation. Angew Chem Int Ed 2011, 50:310-313.
16. Bon M. A discourse upon usefulness of the silk of spiders. Philos Trans 1710, 27:2-16.
17. Allmeling C., Jokuszies A., Reimers K., Kall S., Choi C.Y., Brandes G., Kasper C., Scheper T., Guggenheim M., Vogt P.M. Spider silk fibres in artificial nerve constructs promote peripheral nerve regeneration. Cell Prolif 2008, 41:408-420.
18. Allmeling C., Jokuszies A., Reimers K., Kall S., Vogt P.M. Use of spider silk fibres as an innovative material in a biocompatible artificial nerve conduit. J Cell Mol Med 2006, 10:770-777.
19. Radtke C., Allmeling C., Waldmann K.H., Reimers K., Thies K., Schenk H.C., Hillmer A., Guggenheim M., Brandes G., Vogt P.M. Spider silk constructs enhance axonal regeneration and remyelination in long nerve defects in sheep. PLoS ONE 2011, 6:e16990.
20. Hennecke K., Redeker J., Kuhbier J.W., Strauss S., Allmeling C., Kasper C., Reimers K., Vogt P.M. Bundles of spider silk, braided into sutures, resist basic cyclic tests: potential use for flexor tendon repair. PLoS ONE 2013, 8:e61100.
21. Kuhbier J.W., Reimers K., Kasper C., Allmeling C., Hillmer A., Menger B., Vogt P.M., Radtke C. First investigation of spider silk as a braided microsurgical suture. J Biomed Mater Res B Appl Biomater 2011, 97:381-387.
22. Madsen B., Shao Z.Z., Vollrath F. Variability in the mechanical properties of spider silks on three levels: interspecific, intraspecific and intraindividual. Int J Biol Macromol 1999, 24:301-306.
23. Fox L.R. Cannibalism in natural-populations. Annu Rev Ecol System 1975, 6:87-106.
24. Hardy J.G., Romer L.M., Scheibel T.R. Polymeric materials based on silk proteins. Polymer 2008, 49:4309-4327.
25. Spiess K., Wohlrab S., Scheibel T. Structural characterization and functionalization of engineered spider silk films. Soft Matter 2010, 6:4168-4174.
26. Schacht K., Scheibel T. Controlled hydrogel formation of a recombinant spider silk protein. Biomacromolecules 2011, 12:2488-2495.
27. Slotta U.K., Rammensee S., Gorb S., Scheibel T. An engineered spider silk protein forms microspheres. Angew Chem Int Ed 2008, 47:4592-4594.
28. Leal-Egana A., Lang G., Mauerer C., Wickinghoff J., Weber M., Geimer S., Scheibel T. Interactions of fibroblasts with different morphologies made of an engineered spider silk protein. Adv Eng Mater 2012, 14:B67-B75.
29. Hermanson K.D., Huemmerich D., Scheibel T., Bausch A.R. Engineered microcapsules fabricated from reconstituted spider silk. Adv Mater 2007, 19:1810-1815.
30. Wohlrab S., Muller S., Schmidt A., Neubauer S., Kessler H., Leal-Egana A., Scheibel T. Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins. Biomaterials 2012, 33:6650-6659.
31. Bini E., Foo C.W.P., Huang J., Karageorgiou V., Kitchel B., Kaplan D.L. RGD-functionalized bioengineered spider dragline silk biomaterial. Biomacromolecules 2006, 7:3139-3145.
32. Leal-Egana A., Scheibel T. Interactions of cells with silk surfaces. J Mater Chem 2012, 22:14330-14336.
33. Grant D.S., Tashiro K-I., Segui-Real B., Yamada Y., Martin G., Kleinman H. Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell 1989, 58:933-943.
34. Graf J., Iwamoto Y., Sasaki M., Martin G., Kleinman H., Robey F., Yamada Y. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell 1987, 48:989-996.
35. Widhe M., Johansson U., Hillerdahl C.O., Hedhammar M. Recombinant spider silk with cell binding motifs for specific adherence of cells. Biomaterials 2013, 34:8223-8234.
36. Gomes S.C., Leonor I.B., Mano J.F., Reis R.L., Kaplan D.L. Antimicrobial functionalized genetically engineered spider silk. Biomaterials 2011, 32:4255-4266.
37. Gomes S., Gallego-Llamas J., Leonor I.B., Mano J.F., Reis R.L., Kaplan D.L. Biological responses to spider silk-antibiotic fusion protein. J Tissue Eng Regener Med 2011, 6:356-368.
38. Currie H.A., Deschaume O., Naik R.R., Perry C.C., Kaplan D.L. Genetically engineered chimeric silk-silver binding proteins. Adv Funct Mater 2011, 21:2889-2895.
39. Foo C.W.P., Patwardhan S.V., Belton D.J., Kitchel B., Anastasiades D., Huang J., Naik R.R., Perry C.C., Kaplan D.L. Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. Proc Natl Acad Sci U S A 2006, 103:9428-9433.
40. Mieszawska A.J., Fourligas N., Georgakoudi I., Ouhib N.M., Belton D.J., Perry C.C., Kaplan D.L. Osteoinductive silk-silica composite biomaterials for bone regeneration. Biomaterials 2010, 31:8902-8910.
41. Belton D.J., Mieszawska A.J., Currie H.A., Kaplan D.L., Perry C.C. Silk-silica composites from genetically engineered chimeric proteins: materials properties correlate with silica condensation rate and colloidal stability of the proteins in aqueous solution. Langmuir 2012, 28:4373-4381.
42. Numata K., Kaplan D.L. Silk-based gene carriers with cell membrane destabilizing peptides. Biomacromolecules 2010, 11:3189-3195.
43. Gomes S., Numata K., Leonor I.B., Mano J.F., Reis R.L., Kapan D.L. AFM study of morphology and mechanical properties of a chimeric spider silk and bone sialoprotein protein for bone regeneration. Biomacromolecules 2011, 12:1675-1685.
44. Mizuno M., Imai T., Fujisawa R., Tani H., Kuboki Y. Bone sialoprotein (BSP) is a crucial factor for the expression of osteoblastic phenotypes of bone marrow cells cultured on type I collagen matrix. Calcif Tissue Int 2000, 66:388-396.
45. Valverde P., Zhang J., Fix A., Zhu J., Ma W.L., Tu Q.S., Chen J. Overexpression of bone sialoprotein leads to an uncoupling of bone formation and bone resorption in mice. J Bone Miner Res 2008, 23:1775-1788.
46. Gomes S., Leonor I.B., Mano J.F., Reis R.L., Kaplan D.L. Spider silk-bone sialoprotein fusion proteins for bone tissue engineering. Soft Matter 2011, 7:4964-4973.
47. Gomes S., Gallego-Llamas J., Leonor I.B., Mano J.F., Reis R.L., Kaplan D.L. In vivo biological responses to silk proteins functionalized with bone sialoprotein. Macromol Biosci 2013, 13:444-454.
48. Huang J., Wong C., George A., Kaplan D.L. The effect of genetically engineered spider silk-dentin matrix protein 1 chimeric protein on hydroxyapatite nucleation. Biomaterials 2007, 28:2358-2367.
49. Yan W.Q., Nakamura T., Kawanabe K., Nishigochi S., Oka M., Kokubo T. Apatite layer-coated titanium for use as bone bonding implants. Biomaterials 1997, 18:1185-1190.
50. Li P.J. Biomimetic nano-apatite coating capable of promoting bone ingrowth. J Biomed Mater Res A 2003, 66A:79-85.
51. Barrere F., van der Valk C.M., Meijer G., Dalmeijer R.A.J., de Groot K., Layrolle P. Osteointegration of biomimetic apatite coating applied onto dense and porous metal implants in femurs of goats. J Biomed Mater Res B Appl Biomater 2003, 67:655-665.
52. Chou Y.F., Dunn J.C.Y., Wu B.M. In vitro response of MC3T3-E1 preosteoblasts within three-dimensional apatite-coated PLGA scaffolds. J Biomed Mater Res B Appl Biomater 2005, 75:81-90.
53. Pajkos A., Deva A.K., Vickery K., Cope C., Chang L., Cossart Y.E. Detection of subclinical infection in significant breast implant capsules. Plast Reconstruct Surg 2003, 111:1605-1611.
54. Hezi-Yamit A., Sullivan C., Wong J., David L., Chen M.F., Cheng P.W., Shumaker D., Wilcox J.N., Udipi K. Impact of polymer hydrophilicity on biocompatibility: implication for DES polymer design. J Biomed Mater Res A 2009, 90:133-141.
55. Henriksen T.F., Fryzek J.P., Holmich L.R., McLaughlin J.K., Kjoller K., Hoyer A.P., Olsen D.H., Friis S. Surgical intervention and capsular contracture after breast augmentation - a prospective study of risk factors. Ann Plast Surg 2005, 54:343-351.
56. Dancey A., Nassimizadeh A., Levick P. Capsular contracture - what are the risk factors? A 14 year series of 1400 consecutive augmentations. J Plast Reconstruct Aesth Surg 2012, 65:213-218.
57. Zeplin P.H., Larena-Avellaneda A., Jordan M., Laske M., Schmidt K. Phosphorylcholine-coated silicone implants effect on inflammatory response and fibrous capsule formation. Ann Plast Surg 2010, 65:560-564.
58. Zeplin P.H., Maksimovikj N.C., Jordan M.C., Nickel J., Lang G., Leimer A.H., Römer L., Scheibel T. Spider silk coatings as a bioshield to reduce periprosthetic fibrous capsule formation. Adv Funct Mater 2014, 10.1002/adfm20130281.
59. Sell S.A., Wolfe P.S., Garg K., McCool J.M., Rodriguez I.A., Bowlin G.L. The use of natural polymers in tissue engineering: a focus on electrospun extracellular matrix analogues. Polymers 2010, 2:522-553.
60. Bauer F., Wohlrab S., Scheibel T. Controllable cell adhesion, growth and orientation on layered silk protein films. Biomater Sci 2013, 1:1244-1249.
61. Agapov I.I., Pustovalova O.L., Moisenovich M.M., Bogush V.G., Sokolova O.S., Sevastyanov V.I., Debabov V.G., Kirpichnikov M.P. Three-dimensional scaffold made from recombinant spider silk protein for tissue engineering. Doklady Biochem Biophys 2009, 426:127-130.
62. Moisenovich M.M., Pustovalova O., Shackelford J., Vasiljeva T.V., Druzhinina T.V., Kamenchuk Y.A., Guzeev V.V., Sokolova O.S., Bogush V.G., Debabov V.G., et al. Tissue regeneration in vivo within recombinant spidroin 1 scaffolds. Biomaterials 2012, 33:3887-3898.
63. Widhe M., Bysell H., Nystedt S., Schenning I., Malmsten M., Johansson J., Rising A., Hedhammar M. Recombinant spider silk as matrices for cell culture. Biomaterials 2010, 31:9575-9585.
64. Fredriksson C., Hedhammar M., Feinstein R., Nordling K., Kratz G., Johansson J., Huss F., Rising A. Tissue response to subcutaneously implanted recombinant spider silk: an in vivo study. Materials 2009, 2:1908-1922.
65. Yang L.A., Hedhammar M., Blom T., Leifer K., Johansson J., Habibovic P., van Blitterswijk C.A. Biomimetic calcium phosphate coatings on recombinant spider silk fibres. Biomed Mater 2010, 5:1-10.
66. Lammel A., Schwab M., Hofer M., Winter G., Scheibel T. Recombinant spider silk particles as drug delivery vehicles. Biomaterials 2011, 32:2233-2240.
67. Hofer M., Winter G., Myschik J. Recombinant spider silk particles for controlled delivery of protein drugs. Biomaterials 2012, 33:1554-1562.
68. Blüm C., Scheibel T. Control of drug loading and release properties of spider silk sub-microparticles. BioNanoSci 2012, 2:67-74.
69. Numata K., Mieszawska-Czajkowska A.J., Kvenvold L.A., Kaplan D.L. Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery. Macromol Biosci 2012, 12:75-82.
70. Numata K., Reagan M.R., Goldstein R.H., Rosenblatt M., Kaplan D.L. Spider silk-based gene carriers for tumor cell-specific delivery. Bioconjug Chem 2011, 22:1605-1610.
71. Blüm C., Nichtl A., Scheibel T. Spider silk capsules as protective reaction containers for enzymes. Adv Funct Mater 2014, 24:763-768.