Cystathionine γ-lyase, an enzyme related to the reverse transsulfuration pathway, is functional in Leishmania spp.

Journal of Eukaryotic Microbiology

Volumn 61, Issue 2, 2014, Pages 204-213

  Giordana, Lucila ^a   Mantilla, Brian Suárez ^b   Santana, Marianela ^a   Silber, Ariel M ^b   Nowicki, Cristina ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

DL propargylglycine; Leishmania parasites

Indexed keywords

COEXISTENCE; ENZYME ACTIVITY; IDENTIFICATION METHOD; MICROBIAL ACTIVITY; PARASITE; PATHOGEN; PHENOTYPE; PROTIST;

ALKYNE; CYSTATHIONINE GAMMA LYASE; CYSTEINE; DRUG DERIVATIVE; ENZYME INHIBITOR; GLYCINE; PROPARGYLGLYCINE; SULFUR;

ARTICLE; BIOSYNTHESIS; DL-PROPARGYLGLYCINE; ENZYMOLOGY; GENETIC COMPLEMENTATION; GENETICS; IC 50; LEISHMANIA MAJOR; LEISHMANIA PARASITES; METABOLISM; SACCHAROMYCES CEREVISIAE;

DL-PROPARGYLGLYCINE; LEISHMANIA PARASITES;

ALKYNES; BIOSYNTHETIC PATHWAYS; CYSTATHIONINE GAMMA-LYASE; CYSTEINE; ENZYME INHIBITORS; GENETIC COMPLEMENTATION TEST; GLYCINE; INHIBITORY CONCENTRATION 50; LEISHMANIA MAJOR; SACCHAROMYCES CEREVISIAE; SULFUR;

EID: 84896372949     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/jeu.12100     Document Type: Article

References (31)

1. Ali, V., &, Nozaki, T., 2007. Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites. Clin. Microbiol. Rev., 20: 164-187.
2. Burke, D., Dawson, D., &, Stearns, T., 2000. Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
3. Cazzulo, J. J., Franke de Cazzulo, B. M., Engel, J. C., &, Cannata, J. J. B., 1985. End products and enzyme levels of aerobic glucose fermentation in Trypanosomatids. Mol. Biochem. Parasitol., 16: 329-343.
4. Croft, S. L., &, Olliaro, P., 2011. Leishmaniasis chemotherapy-challenges and opportunities. Clin. Microbiol. Infect., 17: 1478-1483.
5. Field, M. C., Allen, C. L., Dhir, V., Goulding, D., Hall, B. S., Morgan, G. W., Veazey, P., &, Engstler, M., 2004. New approaches to the microscopic imaging of Trypanosoma brucei. Microsc. Microanal., 10: 621-636.
6. Gaitonde, M., 1967. A spectrophotometric method for direct determination of cysteine Biochem. J., 104: 627-633.
7. Gietz, D., St Jean, A., Woods, R. A., &, Schiestl, R. H., 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res., 20: 1425.
8. Krauth-Siegel, R. L., &, Comini, M. A., 2008. Redox control in trypanosomatids, parasitic protozoa with trypanothione-based thiol metabolism. Biochim. Biophys. Acta, 1780: 1236-1248.
9. Leroux, A., Fleming-Canepa, X., Aranda, A., Maugeri, D., Cazzulo, J. J., Sánchez, M. A., &, Nowicki, C., 2006. Functional characterization and subcellular localization of the three malate dehydrogenase isozymes in Leishmania spp. Mol. Biochem. Parasitol., 149: 74-85.
10. Magdaleno, A., Ahn, I. Y., Paes, L. S., &, Silber, A. M., 2009. Actions of proline analogue, L-thiazolidine-4-carboxylic acid (T4C), on Trypanosoma cruzi. PLoS ONE, 4: e4534-e4544.
11. Marciano, D., Maugeri, D. A., Cazzulo, J. J., &, Nowicki, C., 2009. Functional characterization of stage-specific aminotransferases from trypanosomatids. Mol. Biochem. Parasitol., 166: 172-182.
12. Marciano, D., Santana, M., Mantilla, B. S., Silber, A. M., Marino-Buslje, C., &, Nowicki, C., 2010. Biochemical characterization of serine acetyltransferase and cysteine desulfhydrase from Leishmania major. Mol. Biochem. Parasitol., 173: 170-174.
13. Marciano, D., Santana, M., &, Nowicki, C., 2012. Functional characterization of enzymes involved in cysteine biosynthesis and H(2)S production in Trypanosoma cruzi. Mol. Biochem. Parasitol., 185: 114-120.
14. Medina-Acosta, E., &, Cross, G. A., 1993. Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure. Mol. Biochem. Parasitol., 59: 327-329.
15. Messerschmidt, A., Worbs, M., Steegborn, C., Wahl, M. C., Huber, R., Laber, B., &, Clausen, T., 2003. Determinants of enzymatic specificity in the Cys-Met-metabolism PLP-dependent enzymes family: crystal structure of cystathionine gamma-lyase from yeast and intrafamiliar structure comparison. Biol. Chem., 384: 373-386.
16. Mozzarelli, A., Bettati, B., Campanini, B., Salsi, E., Raboni, S., Singh, R., Spyrakis, F., Kumar, V. P., &, Cook, P. F., 2011. The multifaceted pyridoxal 5′-phosphate-dependent O-acetylserine sulfhydrylase. Biochim. Biophys. Acta, 1814: 1497-1510.
17. 2S signals through protein S-sulfhydration. Sci. Signal, 2: ra72.
18. Nozaki, T., Ali, V., &, Tokoro, M., 2005. Sulfur-containing amino acid metabolism in parasitic protozoa. Adv. Parasitol., 60: 1-99.
19. Nozaki, T., Shigeta, Y., Saito-Nakano, Y., Imada, M., &, Kruger, W., 2001. Characterization of transsulfuration and cysteine biosynthetic pathways in the protozoan hemoflagelate, Trypanosoma cruzi. J. Biol. Chem., 276: 6516-6523.
20. Paape, D., Barrios-Llerena, M. E., Le Bihan, T., Mackay, L., &, Aebischer, T., 2010. Gel free analysis of the proteome of intracellular Leishmania mexicana. Mol. Biochem. Parasitol., 169: 108-114.
21. Pastore, A., &, Piemonte, F., 2012. S-Glutathionylation signaling in cell biology: progress and prospects. Eur. J. Pharm. Sci., 46: 279-292.
22. Piñeyro, M. D., Parodi-Talice, A., Portela, M., Arias, D. G., Guerrero, S. A., &, Robello, C., 2011. Molecular characterization and interactome analysis of Trypanosoma cruzi tryparedoxin 1. J. Proteomics, 74: 1683-1692.
23. Steegborn, C., Clausen, T., Sondermann, P., Jacob, U., Worbs, M., Marinkovic, S., Huber, R., &, Wahl, M. C., 1999. Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration. J. Biol. Chem., 274: 12675-12684.
24. 2S. J. Biol. Chem., 284: 3076-3085.
25. Thomas, D., &, Surdin-Kerjan, Y., 1997. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev., 61: 503-532.
26. Vickers, T. J., Orsomando, G., de la Garza, R. D., Scott, D. A., Kang, S. O., Hanson, A. D., &, Beverley, S. M., 2006. Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence. J. Biol. Chem., 281: 38150-38158.
27. Walker, J., Vasquez, J. J., Gomez, M. A., Drummelsmith, J., Burchmore, R., Girard, I., &, Ouellette, M., 2006. Identification of developmentally-regulated proteins in Leishmania panamensis by proteome profiling of promastigotes and axenic amastigotes. Mol. Biochem. Parasitol., 147: 64-73.
28. WHO 2010. Control of the Leishmaniasis: report of a meeting of the WHO. Expert Committee on the Control of Leishmaniases. WHO Technical Report Series No. 949. WHO, Geneva.
29. Williams, R. A., Westrop, G. D., &, Coombs, G. H., 2009. Two pathways for cysteine biosynthesis in Leishmania major. Biochem. J., 420: 451-462.
30. Wirtz, M., &, Hell, R., 2006. Functional analysis of the cysteine synthase protein complex from plants: structural, biochemical and regulatory properties. J. Plant Physiol., 163: 273-286.
31. Yamagata, S., D'Andrea, R. J., Fujisaki, S., Isaji, M., &, Nakamura, K., 1993. Cloning and bacterial expression of the CYS3 gene encoding cystathionine γ-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the protein. J. Bacteriol., 175: 4800-4808.