Biochemical characterization of serine acetyltransferase and cysteine desulfhydrase from Leishmania major

Molecular and Biochemical Parasitology

Volumn 173, Issue 2, 2010, Pages 170-174

  Marciano, Daniela ^a   Santana, Marianela ^a   Mantilla, Brian Suárez ^b   Silber, Ariel Mariano ^b   Marino Buslje, Cristina ^a,b   Nowicki, Cristina ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cysteine biosynthesis; Cysteine desulfhydrase; Leishmania; Serine acetyltransferase; Transsulfuration pathways

Indexed keywords

BACTERIAL ENZYME; CYSTATHIONINE GAMMA LYASE; SERINE ACETYLTRANSFERASE; CYSTEINE; PROTOZOAL PROTEIN; PYRUVIC ACID;

ARTICLE; ENZYME ANALYSIS; ENZYME SYNTHESIS; LEISHMANIA MAJOR; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE VIRULENCE; PRIORITY JOURNAL; PROTEIN FOLDING; TERMINAL SEQUENCE; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; ENERGY METABOLISM; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CYSTATHIONINE GAMMA-LYASE; CYSTEINE; ENERGY METABOLISM; LEISHMANIA MAJOR; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; PROTOZOAN PROTEINS; PYRUVIC ACID; SEQUENCE HOMOLOGY; SERINE O-ACETYLTRANSFERASE;

BACTERIA (MICROORGANISMS); LEISHMANIA MAJOR;

EID: 77954757372     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2010.06.004     Document Type: Article

References (19)

1. Croft S.L., Sundar S., Fairlamb A.H. Drug resistance in leishmaniasis. Clin Microbiol Rev 2006, 19:111-126.
2. Williams R.A., Westrop G.D., Coombs G.H. Two pathways for cysteine biosynthesis in Leishmania major. Biochem J 2009, 420:451-462.
3. Wirtz M., Hell R. Functional analysis of the cysteine synthase protein complex from plants: structural, biochemical and regulatory properties. J Plant Physiol 2006, 163:273-286.
4. Ali V., Nozaki T. Current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by " amitochondriate" protozoan parasites. Clin Microbiol Rev 2007, 20:164-187.
5. Bzymek K.P., Newton G.L., Ta P., Fahey R.C. Mycothiol import by Mycobacterium smegmatis and function as a resource for metabolic precursors and energy production. J Bacteriol 2007, 189:6796-6805.
6. Mihara H., Kurihara T., Yoshimura T., Soda K., Esaki N. Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities: gene cloning, purification, and characterization of a novel pyridoxal enzyme. J Biol Chem 1997, 272:22417-22424.
7. Fraser R.D.B., Suzuki E. The use of least squares in the data analysis. Physic. Princ. and Tech. of Protein. Chem., vol. 21 1973, 301-355. S.J. Leach (Ed.).
8. Clausen T., Huber R., Messerschmidt A., Pohlenz H., Laber B. Slow-binding inhibition of Escherichia coli cystathionine β-lyase by l-aminoethoxyvinylglycine: a kinetic and X-ray study. Biochemistry 1997, 36:12633-12643.
9. Yoshida Y., Nakano Y., Amano A., et al. lcd from Streptococcus anginosus encodes a C-S lyase with alpha, beta-elimination activity that degrades l-cysteine. Microbiology 2002, 148:3961-3970.
10. Bloem E., Haneklaus S., Salac I., Wickenhäuser P., Schnug E. Facts and fiction about sulfur metabolism in relation to plant-pathogen interactions. Plant Biol (Stuttg) 2007, 9:596-607.
11. Ruffet N., Droux M., Douce R. Purification and kinetic properties of serine acetyltransferase free of O-acetylserine(thiol)lyase from spinach chloroplast. Plant Physiol 1994, 104:597-604.
12. Wirtz M., Hell R. Dominant-negative modification reveals the regulatory function of the multimeric cysteine synthase protein complex in transgenic tobacco. Plant Cell 2007, 2:625-639.
13. Kredich N.M., Tomkins G.M. The enzymic synthesis of l-cysteine in Escherichia coli and Salmonella typhimurium. J Biol Chem 1966, 241:4955-4965.
14. Kumaran S., Yi H., Krishnan H.B., Jez J.M. Assembly of the cysteine synthase complex and the regulatory role of protein-protein interactions. J Biol Chem 2009, 284:10268-10275.
15. Bonner E., Cahoon R., Knapke S., Jez J. Molecular basis of cysteine biosynthesis in plants. Structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. J Biol Chem 2005, 280:38803-38813.
16. Laskowski R.A., MacArthur M.W., Moss D.S., Thornton J.M. PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Crystallogr 1993, 26:283-291.
17. Kumaran S., Jez J.M. Structural basis for interaction of O-acetylserine sulfhydrylase and serine acetyltransferase in the Arabidopsis cysteine synthase complex. Plant Cell 2006, 18:3647-3655.
18. Arnold K., Bordoli L., Kopp J., Schwede T. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 2006, 22:195-201.
19. Pettersen E.F., Goddard T.D., Huang C.C., et al. Chimera: a visualization system for exploratory research and analysis. J Comput Chem 2004, 13:1605-1612.