Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells

Ultramicroscopy

Volumn 143, Issue , 2014, Pages 3-14

  Peddie, Christopher J ^a   Blight, Ken ^a   Wilson, Emma ^a   Melia, Charlotte ^a,c   Marrison, Jo ^b   Carzaniga, Raffaella ^a   Domart, Marie Charlotte ^a   O'Toole, Peter ^b   Larijani, Banafshe ^a,d,e   Collinson, Lucy M ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

^c LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^d CSIC UPV Unidad de Biofisica UBF   (Spain)

^e BASQUE FOUNDATION FOR SCIENCE   (Spain)

Author keywords

Correlative light and electron microscopy; Diacylglycerol; GFP; In resin fluorescence; Integrated light and electron microscopy; Mammalian cells

Indexed keywords

CYTOLOGY; FLUORESCENCE MICROSCOPY; GLYCEROL; LIPIDS; RESINS; SCANNING ELECTRON MICROSCOPY; TOOLS;

DIACYLGLYCEROL; FUNCTIONAL INFORMATION; FUNDAMENTAL TOOLS; GFP; INTEGRATED IMAGING; MAMMALIAN CELLS; MEMBRANE MORPHOLOGY; SUB-CELLULAR LOCALISATION;

FLUORESCENCE;

DIACYLGLYCEROL; GREEN FLUORESCENT PROTEIN; RESIN; 1,2-DIACYLGLYCEROL; NUCLEOPLASMIN;

ARTICLE; CELL NUCLEUS MEMBRANE; CELL ORGANELLE; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CORRELATIVE LIGHT AND ELECTRON MICROSCOPY; FEMALE; FLUORESCENCE; FREEZING; GOLGI COMPLEX; HUMAN; HUMAN CELL; IN RESIN FLUORESCENCE; INTEGRATED LIGHT AND ELECTRON MICROSCOPY; MAMMAL CELL; MEASUREMENT ACCURACY; MICROSCOPY; NUCLEOPLASMIC RETICULUM; VALIDATION STUDY; ANIMAL; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; HELA CELL LINE; LIGHT; MAMMAL; METABOLISM; PROCEDURES; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; DIGLYCERIDES; FLUORESCENCE; GOLGI APPARATUS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LIGHT; MAMMALS; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; NUCLEAR ENVELOPE; NUCLEOPLASMINS;

EID: 84895728462     PISSN: 03043991     EISSN: 18792723     Source Type: Journal    
DOI: 10.1016/j.ultramic.2014.02.001     Document Type: Article

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