Confocal/TEM overlay microscopy: A simple method for correlating confocal and electron microscopy of cells expressing GFP/YFP fusion proteins

Microscopy and Microanalysis

Volumn 14, Issue 4, 2008, Pages 342-348

  Keene, Douglas R ^a,b   Tufa, Sara F ^a   Lunstrum, Gregory P ^a   Holden, Paul ^a   Horton, William A ^a,b  

^a SHRINERS HOSPITAL FOR CHILDREN   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Adobe Photoshop; Cell culture; Confocal transmission overlay (CTO) microscopy; Correlative microscopy; Method; Technique; Transmission electron microscopy

Indexed keywords

HYBRID PROTEIN; PHOTOPROTEIN;

ANIMAL; ARTICLE; CELL; CHEMISTRY; CHO CELL; CONFOCAL MICROSCOPY; CRICETULUS; ELECTRON MICROSCOPY; HAMSTER; METHODOLOGY; RAT; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CELLS; CHO CELLS; CRICETINAE; CRICETULUS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; RATS; RECOMBINANT FUSION PROTEINS;

EID: 46749143349     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927608080306     Document Type: Article

References (12)

1. BIEL, S.S., KAWASCHINSKI, K., WITTERN, K.-P., HINTZE, U. & WEPF, R. (2003). From tissue to cellular ultrastructure: Closing the gap between micro- and nanostructural imaging. J Microsc 212, 91-99.
2. BRORSON, S.-H. (1998). Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin. Histol Histopathol 13, 275-281.
3. CHO, J.Y., GUO, C., TORELLO, M., LUNSTRUM, G.P., IWATA, T., DENG, C. & HORTON, W.A. (2004). Defective lysosomal targeting of activated fibroblast growth factor receptor 3 in achondroplasia. Proc Natl Acad Sci 101, 609-614.
4. GRABENBAUER, M., GEERTS, W.J., FERNANDEZ- RODRIGUEZ, J., HOENGER, A., KOSTER, A.J. & NILSSON, T. (2005). Correlative microscopy and electron tomography of GFP through photooxidation. Nat Methods 2, 857-862.
5. GROOS, S., REALE, E. & LUCIANO, L. (2001). Re-evaluation of epoxy resin sections for light and electron microscopic immunostaining. J Histochem Cytochem 49, 397-406.
6. HOLDEN, P., KEENE, D.R., LUNSTRUM, G.P., BÄCHINGER, H.P. & HORTON, W.A. (2005). Secretion of cartilage oligomeric matrix protein is affected by the signal peptide. J Biol Chem 280, 17172-17179.
7. LUBY-PHELPS, K., NING, G., FOGERTY, J. & BESHARSE, J.C. (2003). Visualization of identified GFP-expressing cells by light and electron microscopy. J Histochem Cytochem 51, 271-274.
8. MOROZOV, Y., KHALILOV, I., YEHEZKEL, B. & REPRESA, A. (2002). Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure. J Neurosci Methods 117, 81-85.
9. PAWLEY, J.B. (1995). Handbook of Biological Confocal Microscopy. New York: Plenum Press.
10. POMBO, A., HOLLINSHEAD, M. & COOK, P.R. (1999). Bridging and resolution gap: Imaging the same transcription factories in cryosections by light and electron microscopy. J Histochem Cytochem 47, 471-480.
11. REN, Y., KRUHLAK, M.J. & BAZETT- JONES, D.P. (2003). Same serial section correlative light and energy-filtered transmission electron microscopy. J Histochem Cytochem 51, 605-612.
12. SIMS, P.A. & HARDIN, J.D. (2007). Fluorescence-integrated transmission electron microscopy images: Integrating fluorescence microscopy with transmission electron microscopy. Methods Mol Biol 369, 291-308.