Resveratrol inhibits hydrogen peroxide-induced apoptosis in endothelial cells via the activation of PI3K/Akt by miR-126

Journal of Atherosclerosis and Thrombosis

Volumn 21, Issue 2, 2014, Pages 108-118

  Sui, Xiang Qian ^a   Xu, Zheng Ming ^a   Xie, Ming Bin ^a   Pei, De An ^a  

^a Department of Cardiology Hangzhou Red Cross Hospital ^*  (China)

Author keywords

Endothelial cells; miR 126; Oxidative stress; PI3K Akt; Resveratrol

Indexed keywords

CASPASE 3; HYDROGEN PEROXIDE; MICRORNA 126; PROTEIN P85; RESVERATROL;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; ENDOTHELIUM CELL; FLOW CYTOMETRY; GENE EXPRESSION; GENE OVEREXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; MTT ASSAY; OXIDATIVE STRESS; PROTEIN PHOSPHORYLATION; REAL TIME POLYMERASE CHAIN REACTION; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION; RNA INTERFERENCE; WESTERN BLOTTING;

ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; APOPTOSIS; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; HYDROGEN PEROXIDE; MICRORNAS; OXIDANTS; OXIDATIVE STRESS; PHOSPHATIDYLINOSITOL 3-KINASES; PROTO-ONCOGENE PROTEINS C-AKT; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STILBENES;

EID: 84894615699     PISSN: 13403478     EISSN: 18803873     Source Type: Journal    
DOI: 10.5551/jat.19257     Document Type: Article

References (29)

1. Gersh BJ, Sliwa K, Mayosi BM, Yusuf S: Novel therapeutic concepts: the epidemic of cardiovascular disease in the developingworld: global implications. Eur Heart J, 2010; 31: 642-648.
2. Plutzky J: Peroxisome proliferator-activated receptors in vascular biology and atherosclerosis: emerging insights for evolving paradigms. Curr Atheroscler Rep, 2000; 2: 327-335.
3. Cai H: Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences. Cardiovasc Res, 2005; 68: 26-36.
4. Bayraktutan U: Free radicals, diabetes and endothelial dysfunction. Diabetes Obes Metab, 2002; 4: 224-238.
5. Harikumar KB, Aggarwal BB: Resveratrol: a multitargeted agent for age-associated chronic diseases. Cell Cycle, 2008; 7: 1020-1035.
6. Borriello A, Cucciolla V, Della Raqione F, Galletti P: Dietary polyphenols: Focus on resveratrol, a promising agent in the prevention of cardiovascular diseases and control of glucose homeostasis. Nutr Metab Cardiovasc Dis, 2010; 20: 618-625.
7. Albani D, Polito L, Forloni G: Sirtuins as novel targets for Alzheimer's disease and other neurodegenerative disorders: experimental and genetic evidence. J Alzheimers Dis, 2010; 19: 11-26.
8. Kao CL, Chen LK, Chang YL, Yung MC, Hsu CC, Chen YC, Lo WL, Chen SJ, Ku HH, Hwang SJ: Resveratrol protects human endothelium from H(2)O(2)-induced oxidative stress and senescence via SirT1 activation. J Atheroscler Thromb, 2010; 17: 970-979.
9. Spanier G, Xu H, Xia N, Tobias S, Deng S, Wojnowski L, Forstermann U, Li H: Resveratrol reduces endothelial oxidative stress by modulating gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4). J Physiol Pharmacol, 2009; 60 Suppl 4: 111-116.
10. Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell, 2004; 116: 281-297.
11. Santoro MM, Nicoli S: miRNAs in endothelial cell signaling: The endomiRNAs. Exp Cell Res, 2012; S0014-4827: 00486-7.
12. Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, Ivey KN, Bruneau BG, Stainier DY, Srivastava D: miR-126 regulates angiogenic signaling and vascular integrity. Dev Cell, 2008; 15: 272-284.
13. Meng S, Cao JT, Zhang B, Zhou Q, Shen CX, Wang CQ: Downregulation of microRNA-126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred-1. J Mol Cell Cardiol, 2012; 53: 64-72.
14. Almhanna K, Strosberg J, Malafa M: Targeting AKT protein kinase in gastric cancer. Anticancer Res, 2011; 31: 4387-4392.
15. Yang P, Peairs JJ, Tano R, Jaffe GJ: Oxidant-mediated Akt activation in human RPE cells. Invest Ophthalmol Vis Sci, 2006; 47: 4598-4606.
16. Vermes I, Haanen C, Reutelingsperger C: Flow cytometry of apoptotic cell death. J Immunol Methods, 2000; 243: 167-190.
17. Riedl SJ, Shi Y: Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol, 2004; 5: 897-907.
18. Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, Ivey KN, Bruneau BG, Stainier DY, Srivastava D. miR-126 regulates angiogenic signaling and vascular integrity. Dev Cell, 2008; 15: 272-284.
19. Harris TA, Yamakuchi M, Kondo M, Oettgen P, Lowenstein CJ: Ets-1 and Ets-2 regulate the expression of microRNA-126 in endothelial cells. Arterioscler Thromb Vasc Biol, 2010; 30: 1990-1997.
20. Spanier G, Xu H, Xia N, Tobias S, Deng S, Wojnowski L, Forstermann U, Li H: Resveratrol reduces endothelial oxidative stress by modulating the gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4). J Physiol Pharmacol, 2009; 60 Suppl 4: 111-116.
21. Rubiolo JA, Mithieux G, Vega FV: Resveratrol protects primary rat hepatocytes against oxidative stress damage: activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. Eur J Pharmacol, 2008; 591: 66-72.
22. Wang S, Aurora AB, Johnson BA, Qi X, McAnally J, Hill JA, Richardson JA, Bassel-Duby R, Olson EN: The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis. Dev Cell, 2008; 15: 261-271.
23. Noratto GD, Angel-Morales G, Talcott ST, Mertens-Talcott SU: Polyphenolics protect human umbilical vascular Endothelial cells (HUVEC) from glucose-and lipopolysaccharide (LPS)-induced inflammation and target microRNA-126. J Agric Food Chem, 2011; 59: 7999-8012.
24. Yang J, Lan H, Huang X, Liu B, Tong Y: MicroRNA-126 inhibits tumor cell growth and its expression level correlates with poor survival in non-small cell lung cancer patients. PLoS One, 2012; 7: e42978.
25. Meng S, Cao JT, Zhang B, Zhou Q, Shen CX, Wang CQ: Downregulation of microRNA-126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred-1. J Mol Cell Cardiol, 2012; 53: 64-72.
26. Ueki K, Fruman DA, Yballe CM, Fasshauer M, Klein J, Asano T, Cantley LC, Kahn CR: Positive and negative roles of p85 alpha and p85 beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling. J Biol Chem, 2003; 278: 48453-48466.
27. Fujio Y, Walsh K: Akt mediates cytoprotection of endothelial cells by vascular endothelial growth factor in an anchorage-dependent manner. J Biol Chem, 1999; 274: 16349-16354.
28. Yang B, Oo TN, Rizzo V: Lipid rafts mediate H2O2 prosurvival effects in cultured endothelial cells. FASEB J, 2006; 20: 1501-1503.
29. Hu L, Sun Y, Hu J: Catalpol inhibits apoptosis in hydrogen peroxide-induced endothelium by activating the PI3K/Akt signaling pathway and modulating expression of Bcl-2 and Bax. Eur J Pharmacol, 2010; 628: 155-163