Structural insights into the catalytic mechanism of human squalene synthase

Acta Crystallographica Section D: Biological Crystallography

Volumn 70, Issue 2, 2014, Pages 231-241

  Liu, Chia I ^a,b,c   Jeng, Wen Yih ^d   Chang, Wei Jung ^a,b   Shih, Min Fang ^a,b   Ko, Tzu Ping ^a,b   Wang, Andrew H J ^a,b,c  

^a INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^b ACADEMIA SINICA   (Taiwan)

^c TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^d NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

cholesterol synthesis; farnesyl pyrophosphate; nicotinamide adenine dinucleotide; squalene synthase; terpenoids

Indexed keywords

DIVALENT CATION; FARNESYL DIPHOSPHATE; ISOPRENOID PHOSPHATE; MAGNESIUM; MANGANESE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PRESQUALENE PYROPHOSPHATE; RECOMBINANT PROTEIN; SESQUITERPENE; SQUALENE; SQUALENE SYNTHASE;

ARTICLE; BIOCATALYSIS; CHEMISTRY; CHOLESTEROL SYNTHESIS; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STATIC ELECTRICITY; TERPENOIDS; X RAY CRYSTALLOGRAPHY;

CHOLESTEROL SYNTHESIS; FARNESYL PYROPHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE; SQUALENE SYNTHASE; TERPENOIDS;

BIOCATALYSIS; CATALYTIC DOMAIN; CATIONS, DIVALENT; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; GENE EXPRESSION; HUMANS; MAGNESIUM; MANGANESE; MUTAGENESIS, SITE-DIRECTED; NADP; POLYISOPRENYL PHOSPHATES; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SESQUITERPENES; SQUALENE; STATIC ELECTRICITY;

EID: 84894194357     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S1399004713026230     Document Type: Article

References (38)

1. Akamine, S., Nakamori, K., Chechetka, S. A., Banba, M., Umehara, Y., Kouchi, H., Izui, K. & Hata, S. (2003). Biochim. Biophys. Acta, 1626, 97-101.
2. Blagg, B. S., Jarstfer, M. B., Rogers, D. H. & Poulter, C. D. (2002). J. Am. Chem. Soc. 124, 8846-8853.
3. Busquets, A., Keim, V., Closa, M., Del Arco, A., Boronat, A., Arro, M. & Ferrer, A. (2008). Plant Mol. Biol. 67, 25-36.
4. Cao, R., Zhang, Y, Mann, F. M., Huang, C, Mukkamala, D., Hudock, M. P., Mead, M. E., Prisic, S., Wang, K., Lin, F.-Y, Chang, T.-K., Peters, R. J. & Oldfield, E. (2010). Proteins, 78, 2417-2432.
5. Dogbo, O., Laferriere, A., D'Harlingue, A. & Camara, B. (1988). Proc. Natl Acad. Sci. USA, 85, 7054-7058.
6. Emsley, P & Cowtan, K. (2004). Acta Cryst. D60, 2126-2132.
7. Gu, P., Ishii, Y, Spencer, T. A. & Shechter, I. (1998). J. Biol. Chem. 273, 12515-12525.
8. Guo, R.-T., Cao, R., Liang, P. H., Ko, T.-P, Chang, T.-H., Hudock, M. P., Jeng, W.-Y, Chen, C. K.-M., Zhang, Y, Song, Y, Kuo, C.-J., Yin, F., Oldfield, E. & Wang, A. H.-J. (2007). Proc. Natl Acad. Sci. USA, 104, 10022-10027.
9. Hyatt, D. C, Youn, B., Zhao, Y, Santhamma, B., Coates, R. M., Croteau, R. B. & Kang, C (2007). Proc. Natl Acad. Sci. USA, 104, 5360-5365.
10. Iwata-Reuyl, D., Math, S. K., Desai, S. B. & Poulter, C. D. (2003). Biochemistry, 42, 3359-3365.
11. Jarstfer, M. B., Zhang, D.-L. & Poulter, C. D. (2002). J. Am. Chem. Soc. 124, 8834-8845.
12. Kahlon, A. K., Roy, S. & Sharma, A. (2010). J. Biomol. Struct. Dyn. 28, 201-210.
13. Lee, S. & Poulter, C. D. (2008). J. Bacteriol. 190, 3808-3816.
14. Liang, P.-H. (2009). Biochemistry, 48, 6562-6570.
15. Lin, F.-Y., Liu, C.-I., Liu, Y.-L., Zhang, Y., Wang, K., Jeng, W.-Y., Ko, T.-P., Cao, R., Wang, A. H.-J. & Oldfield, E. (2010). Proc. Natl Acad. Sci. USA, 107, 21337-21342.
16. Lin, F.-Y., Zhang, Y., Hensler, M., Liu, Y.-L., Chow, O. A., Zhu, W., Wang, K., Pang, R., Thienphrapa, W., Nizet, V. & Oldfield, E. (2012). ChemMedChem, 7, 561-564.
17. Liu, C.-I., Jeng, W.-Y., Chang, W.-J., Ko, T.-P. & Wang, A. H.-J. (2012). J. Biol. Chem. 287, 18750-18757.
18. Lovell, S. C., Davis, I. W., Arendall, W. B. III, De Bakker, P. I., Word, J. M., Prisant, M. G., Richardson, J. S. & Richardson, D. C. (2003). Proteins, 50, 437-450.
19. Mookhtiar, K. A., Kalinowski, S. S., Zhang, D. & Poulter, C. D. (1994). J. Biol. Chem. 15, 11201-11207.
20. Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Long, F. & Vagin, A. A. (2011). Acta Cryst. D67, 355-367.
21. Nakashima, T., Inoue, T., Oka, A., Nishino, T., Osumi, T. & Hata, S. (1995). Proc. Natl Acad. Sci. USA, 92, 2328-2332.
22. Okada, S., Devarenne, T. P., Murakami, M., Abe, H. & Chappell, J. (2004). Arch. Biochem. Biophys. 422, 110-118.
23. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
24. Pan, J.-J., Bugni, T. S. & Poulter, C. D. (2009). J. Org. Chem. 74, 7562-7565.
25. Pandit, J., Danley, D. E., Schulte, G. K., Mazzalupo, S., Pauly, T. A., Hayward, C. M., Hamanaka, E. S., Thompson, J. F. & Harwood, H. J. Jr (2000). J. Biol. Chem. 275, 30610-30617.
26. Pelz, A., Wieland, K. P., Putzbach, K., Hentschel, P., Albert, K. & Götz, F. (2005). J. Biol. Chem. 280, 32493-32498.
27. Perzl, M., Reipen, I. G., Schmitz, S., Poralla, K., Sahm, H., Sprenger, G. A. & Kannenberg, E. L. (1998). Biochim. Biophys. Acta, 1393, 108-118.
28. Radisky, E. S. & Poulter, C. D. (2000). Biochemistry, 39, 1748-1760.
29. Rilling, H. C. (1966). J. Biol. Chem. 10, 241, 3233-3236.
30. Rondeau, J. M., Bitsch, F., Bourgier, E., Geiser, M., Hemmig, R., Kroemer, M., Lehmann, S., Ramage, P., Rieffel, S., Strauss, A., Green, J. R. & Jahnke, W. (2006). ChemMedChem, 1, 267-273.
31. Starks, C. M., Back, K., Chappell, J. & Noel, J. P. (1997). Science, 277, 1815-1820.
32. Takatsuji, H., Nishino, T., Izui, K. & Katsuki, H. (1982). J. Biochem. 91, 911-921.
33. Vagin, A. & Teplyakov, A. (2010). Acta Cryst. D66, 22-25.
34. Vaguine, A. A., Richelle, J. & Wodak, S. J. (1999). Acta Cryst. D55, 191-205.
35. Wallace, A. C., Laskowski, R. A. & Thornton, J. M. (1995). Protein Eng. 8, 127-134.
36. Winn, M. D. et al. (2011). Acta Cryst. D67, 235-242.
37. Zhang, D., Jennings, S. M., Robinson, G. W. & Poulter, C. D. (1993). Arch. Biochem. Biophys. 304, 133-143.
38. Zhao, R.-Y., Xiao, W., Cheng, H.-L., Zhu, P. & Cheng, K.-D. (2010). J. Ind. Microbiol. Biotechnol. 37, 1171-1182.