Construction of mesenchymal stem cell-containing collagen gel with a macrochanneled polycaprolactone scaffold and the flow perfusion culturing for bone tissue engineering

BioResearch Open Access

Volumn 1, Issue 3, 2012, Pages 124-136

  Yu, Hye Sun ^a,b   Won, Jong Eun ^a,b   Jin, Guang Zhen ^a,b   Kim, Hae Won ^a,b,c  

^a Dankook University   (South Korea)

^b BK21 Plus NBM Global Research Center for Regenerative Medicine   (South Korea)

^c Dankook University   (South Korea)

Author keywords

3D scaffolds; Bone tissue engineering; Collagen gel; Flow perfusion; Osteogenic differentiation

Indexed keywords

BONE SIALOPROTEIN; COLLAGEN GEL; CYCLOOXYGENASE 2; OSTEOCALCIN; OSTEOPONTIN; POLYCAPROLACTONE; PROTEIN C FOS; TISSUE SCAFFOLD;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BONE DEVELOPMENT; BONE TISSUE; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; FORCE TRANSDUCER; GENE EXPRESSION; HYDROGEL; MESENCHYMAL STEM CELL; MINERALIZATION; NONHUMAN; ONCOGENE C FOS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN INDUCTION; RAT; TISSUE ENGINEERING; UPREGULATION; WESTERN BLOTTING;

EID: 84893767601     PISSN: None     EISSN: 21647860     Source Type: Journal    
DOI: 10.1089/biores.2012.0234     Document Type: Article

References (51)

1. Langer R, Vacanti JP. Tissue engineering. Science. 1993; 260:920-926.
2. Laurencin CT, Ambrosis AM, Borden MD, et al. Tissue engineering: orthopedic applications. Annu Rev Biomed Eng. 1999;1:19-46.
3. Hollister SJ. Porous scaffold design for tissue engineering. Nat Mater. 2005;4:518-524.
4. Ikada Y. Challenges in tissue engineering. JR Soc Interface. 2006;3:589-601.
5. Liu X, Ma PX. Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng. 2004;32:477-486.
6. Gunatillake PA, Adhikari R. Biodegradable synthetic polymers for tissue engineering. Eur Cell Mater. 2003;5:1-16.
7. Nair LS, Laurencin CT. Polymers as biomaterials for tissue engineering and controlled drug delivery. Adv Biochem Eng Biotech. 2006;102:47-90.
8. Oh CH, Hong SJ, Yu HS, et al. Development of robotic dispensed bioactive scaffolds and human adipose-derived stem cell culturing for bone tissue engineering. Tissue Eng C. 2010;16:561-571.
9. Weigel T, Schinkel G, Lendlein A. Design and preparation scaffolds for tissue engineering. Expert Rev Med Devices. 2006;3:835-851.
10. Yang S, Leong K, Du Z, et al. The design of scaffolds for use in tissue engineering. Rapid prototyping techniques. Tissue Eng. 2002;8:1-11.
11. Yeong WY, Chua CK, Leong KF, et al. Rapid prototyping in tissue engineering: challenges and potential. Trends Biotech. 2004;22:643-652.
12. Hong SJ, Jeong I, Noh KT, et al. Robotic dispensing of composite scaffolds and in vitro responses of bone marrow stromal cells. J Mater Sci Mater Med. 2009;20:1955-1962.
13. Lee EJ, Vunjak-Novakovic G, Wang Y, et al. A biocompatible endothelial cell delivery system for in vitro tissue engineering. Cell Transplant. 2009;18:731-743.
14. Yang J, Cao C, Wang W, et al. Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture. J Biomed Mater Res A. 2010;92: 817-829.
15. Jeong SI, Kwon JH, Lim JI, et al. Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds. Biomaterials. 2005;26: 1405-1411.
16. Bancroft GN, Sikavitsas VI, Van Den Dolder J, et al. Fluid flow increase mineralized matrix deposition in 3D perfusion culture of marrow stromal ostoblasts in a dose-dependent manner. Proc Natl Acad Sci USA. 2002;99:12600-12605.
17. Kim L, Toh YC, Voldman J, et al. A practical guide to microfluidic perfusion culture of adherent mammalian cells. Lab Chip. 2007;7:681-694.
18. Chen HC, Hu YC. Bioreactors for tissue engineering. Biotechnol Lett. 2006;28:1415-1423.
19. Oh SA, Kim SH, Won JE, et al. Effects on growth and osteogenic differentiation of mesenchymal stem cells by the zincadded sol-gel bioactive glass granules. J Tissue Eng. 2010; DOI:10.4061/2010/475260.
20. Nam SY, Won JE, Kim CH, et al. Odontogenic differentiation of human dental pulp stem cells stimulated by the addition of calcium phosphate porous granules. J Tissue Eng. 2011; DOI:10.4061/2011/812547.
21. -ΔΔDDCT method. Methods. 2001;25:402-408.
22. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21:2529-2543.
23. Godbey WT, Hindy SB, Sherman ME, et al. A novel use of centrifugal force for cell seeding into porous scaffolds. Biomaterials. 2004;25:2799-2805.
24. KimHW, KimHE, SalihV. Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds. Biomaterials. 2005;26:5221-5230.
25. Vunjak-Novakovic G, Obradovic B, Martin I, et al. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotech Prog. 1998;14:193-202.
26. Pu F, Rhodes N, Bayon Y, et al. The use of flow perfusion culture and subcutaneous implantation with fibroblast-seeded PLLA-collagen 3D scaffolds for abdominal wall repair. Biomaterials. 2010;31:4330-4340.
27. Gomes ME, Holtorf HL, Reis RL, et al. Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. Tissue Eng. 2006;12: 801-809.
28. Bancroft GN, Sikavitsas VI, Mikos AG. Technical note: design of a flow perfusion bioreactor system for bone tissueengineering applications. Tissue Eng. 2003;9:549-554.
29. Scherberich A, Galli R, Jaquiery C, et al. Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity. Stem Cells. 2007:25:1823-1829.
30. Cartmell SH, Porter BD, García AJ, et al. Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro. Tissue Eng. 2003;9:1197-1203.
31. Shimizu K, Ito A, Honda H. Mag-seeding of rat bone marrow stromal cells into porous hydroxyapatite scaffolds for bone tissue engineering. J Biosci Bioeng. 2007;104:171-177.
32. Weinand C, Pomerantseva I, Neville CM, et al. Hydrogel-b-TCP scaffolds and stem cells for tissue engineering bone. Bone. 2006;38:555-563.
33. Potier E, Noailly J, Ito K. Directing bone marrow-derived stromal cell function with mechanics. J Biomech. 2010;43: 807-817.
34. Chow JWM, Chambers TJ. Indomethacin has distinct early and late actions on bone formation induced by mechanical stimulation. Am J Physiol. 1994;267:E287-E292.
35. Turner CH, Tu Y, Onyia JE. Mechanical loading of bone in vivo caused bone formation through early induction of cfos, but not c-jun or c-myc. Ann Biomed Eng. 1996;24:S74.
36. Klein-Nulend J, Burger EH, Semeins CM, et al. Pulsating fluid flow stimulates prostaglandin release and inducible prostaglandin G/H synthase mRNA expression in primary mouse bone cells. J Bone Miner Res. 1997;12:45-51.
37. Pavalko FM, Chen NX, Turner CH, et al. Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton- integrin interactions. Am J Physiol Cell Physiol. 1998;275:C1591-C1601.
38. Gardinier JD, Majumdar S, Duncan RL, et al. Cyclic hydraulic pressure and fluid flow differentially modulate cytoskeleton re-organization in MC3T3 osteoblasts. Cell Mol Bioeng. 2009;2:133-143.
39. Hoffman BD, Grashoff C, Schwartz MA. Dynamic molecular processes mediate cellular mechanotransduction. Nature. 475;316-323.
40. Zhang X, Schwarz EM, Young DA, et al. Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair. J Clin Invest. 2002;109:1405-1415.
41. Takai E, Mauck RL, Hung CT, et al. Osteocyte viability and regulation of osteoblast function in a 3D trabecular bone explant under dynamic hydrostatic pressure. J Bone Miner Res. 2004;19:1403-1410.
42. Nagatomi J, Arulanandam BP, Metzger DW, et al. Frequency-and duration dependent effects of cyclic pressure on select bone cell functions. Tissue Eng. 2001;7:717-728.
43. Myers KA, Rattner JB, Shrive NG, et al. Osteoblast-like cells and fluid flow: cytoskeleton dependent shear sensitivity. Biochem Biophys Res Commun. 2007;364:214-219.
44. You J, Yellowley CE, Donahue HJ, et al. Substrate deformation levels associated with routine physical activity are less stimulatory to bone cells relative to loading-induced oscillatory fluid flow. J Biomech Eng. 2000;122:387-393.
45. Brindley D, Moorthy K, Lee JH, et al. Bioprocess forces and their impact on cell behaviour: implications for bone regeneration therapy. J Tissue Eng. 2011; DOI:10.4061/2011/620247.
46. Tanaka SM, Sun HB, Roeder RK, et al. Osteoblast responses one hour after load-induced fluid flow in a three-dimensional porous matrix. Calcif Tissue Int. 2005;76:261-271.
47. Sikavitsas VI, Bancroft GN, Holtorf HL, et al. Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces. Proc Natl Acad Sci USA. 2003;100:14683-14688.
48. Fassina L, Visai L, De Angelis MG, et al. Surface modification of a porous polyurethane through a culture of human osteoblasts and an electromagnetic bioreactor. Technol Health Care. 2007;15:33-45.
49. McCoy RJ, O'Brien FJ. Influence of shear stress in perfusion bioreactor cultures for the development of three-dimensional bone tissue constructs: a review. Tissue Eng Part B Rev. 2010;16:587-601.
50. Weyand B, Israelowitz M, von Schroeder HP, et al. Fluid dynamics in bioreactor design: considerations for the theoretical and practical approach. Adv Biochem Eng Biotechnol. 2009;112:251-268.
51. Rauh J, Milan F, Günther K, et al. Bioreactor systems for bone tissue engineering. Tissue Eng Part B Rev. 2011;17:263-280.