Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Jørgensen, Mathias L ^a   Friis, Niels A ^a   Just, Jesper ^a   Madsen, Peder ^a   Petersen, Steen V ^a   Kristensen, Peter ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Leishmania tarentolae; Protein expression; Recombinant antibodies

Indexed keywords

FC RECEPTOR; GLYCAN; IMMUNOGLOBULIN G; LAMININ; PROTEIN A; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; VIMENTIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CULTURE MEDIUM; LEISHMANIA TARENTOLAE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN STRUCTURE; RABBIT; SUPERNATANT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; LAMININ; LEISHMANIA; MICE; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RABBITS; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES; VIMENTIN;

EID: 84893649007     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-13-9     Document Type: Article

References (42)

1. Carter PJ. Introduction to current and future protein therapeutics: a protein engineering perspective. Exp Cell Res 2011, 317:1261-1269. 10.1016/j.yexcr.2011.02.013, 21371474.
2. Holliger P, Hudson PJ. Engineered antibody fragments and the rise of single domains. Nat Biotech 2005, 23:1126-1136.
3. Hoogenboom HR. Selecting and screening recombinant antibody libraries. Nat Biotechnol 2005, 23:1105-1116. 10.1038/nbt1126, 16151404.
4. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR. Making antibodies by phage display technology. Annu Rev Immunol 1994, 12:433-455. 10.1146/annurev.iy.12.040194.002245, 8011287.
5. Bak Jensen K. Functional improvement of antibody fragments using a novel phage coat protein III fusion system. Biochem Bioph Res Co 2002, 298:566-573.
6. Chen R. Bacterial expression systems for recombinant protein production: E. coli and beyond. Biotechnol Adv 2012, 30:1102-1107. 10.1016/j.biotechadv.2011.09.013, 21968145.
7. Arbabi-Ghahroudi M. Prokaryotic expression of antibodies. Cancer Metast Rev 2005, 24:501-519.
8. Knappik A. The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in escherichia coli. Bio/technology (New York, NY 1983) 1993, 11:77-83.
9. Chan CEZ. Optimized expression of full-length IgG1 antibody in a common E. coli strain. PLoS One 2010, 5:e10261. 10.1371/journal.pone.0010261, 2857744, 20422027.
10. Deyev SM. Multivalency: the hallmark of antibodies used for optimization of tumor targeting by design. Bioessays 2008, 30:904-918. 10.1002/bies.20805, 18693269.
11. Kipriyanov SM. Affinity enhancement of a recombinant antibody: Formation of complexes with multiple valency by a single-chain Fv fragment-core streptavidin fusion. Method Enzymol 1996, 9:203-211.
12. Czajkowsky DM. Fc-fusion proteins: new developments and future perspectives. EMBO Mol Med 2012, 4:1015-1028. 10.1002/emmm.201201379, 3491832, 22837174.
13. Chames P. Therapeutic antibodies: successes, limitations and hopes for the future Therapeutic antibodies: an update. Br J Pharmacol 2009, 157:220-233. 10.1111/j.1476-5381.2009.00190.x, 2697811, 19459844.
14. Hinton PR. An engineered human IgG1 antibody with longer serum half-life. J Immunol (1950) 2006, 176:346-356.
15. Kontermann RE. Strategies to extend plasma half-lives of recombinant antibodies. BioDrugs 2009, 23:93-109. 10.2165/00063030-200923020-00003, 19489651.
16. Hober S. Protein A chromatography for antibody purification. J Chromatogr B 2007, 848:40-47.
17. Akerstrom B. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies. J Immunol (1950) 1985, 135:2589-2592.
18. Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dübel S, Perez F. A multi-Fc-species system for recombinant antibody production. BMC Biotechnol 2009, 9:14. 10.1186/1472-6750-9-14, 2654441, 19245715.
19. Jäger V. High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC Biotechnol 2013, 13:52. 10.1186/1472-6750-13-52, 3699382, 23802841.
20. Frenzel A, Hust M, Schirrmann T. Expression of recombinant antibodies. Front Immunol 2013, 4:Article 217.
21. Yin J. Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J Biotechnol 2007, 127:335-347. 10.1016/j.jbiotec.2006.07.012, 16959350.
22. Niimi T. Recombinant protein production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: a review. Method Mol Biol (Clifton, NJ) 2012, 824:307.
23. Breitling R, Klingner S, Callewaert N, Pietrucha R, Geyer A, Ehrlich G, Hartung R, Müller A, Contreras R, Beverley SM. Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expr Purif 2002, 25:209-218. 10.1016/S1046-5928(02)00001-3, 12135552.
24. Kushnir S, Cirstea IC, Basiliya L, Lupilova N, Breitling R, Alexandrov K. Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae. Mol Biochem Parasitol 2011, 176:69-79. 10.1016/j.molbiopara.2010.12.002, 21167214.
25. Wirtz E. Gene expression mediated by bacteriophage T3 and T7 RNA polymerases in transgenic trypanosomes. Nucleic Acids Res 1994, 22:3887-3894. 10.1093/nar/22.19.3887, 308385, 7937108.
26. Klatt S, Konthur Z. Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae. Microb Cell Fact 2012, 11:97. 10.1186/1475-2859-11-97, 3416730, 22830363.
27. Lu QL. A new blocking method for application of murine monoclonal antibody to mouse tissue sections. J Histochem Cytochem 1998, 46:977-983. 10.1177/002215549804600813, 9671449.
28. Basile G. Recombinant Protein Expression in Leishmania tarentolae. Mol Biotechnol 2009, 43:273-278. 10.1007/s12033-009-9213-5, 19779853.
29. Lee CMY, Iorno N, Sierro F, Christ D. Selection of human antibody fragments by phage display. Nat Protocols 2007, 2:3001-3008.
30. Yamabhai M. Sticky PCR: a PCR-based protocol for targeted protein engineering. Biotechnol J 2009, 4:544-553. 10.1002/biot.200800198, 19370716.
31. de Wildt RMT. Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat Biotechnol 2000, 18:989-994. 10.1038/79494, 10973222.
32. Pansri P. A compact phage display human scFv library for selection of antibodies to a wide variety of antigens. BMC Biotechnol 2009, 9:6. 10.1186/1472-6750-9-6, 2642811, 19175944.
33. Lal A. Clean western blot signals from immunoprecipitated samples. Mol Cell Probes 2005, 19:385-388. 10.1016/j.mcp.2005.06.007, 1350844, 16146684.
34. Rattan SI, Sodagam L. Gerontomodulatory and youth-preserving effects of zeatin on human skin fibroblasts undergoing aging in vitro. Rejuvenation Res 2005, 8:46-57. 10.1089/rej.2005.8.46, 15798374.
35. Wan L. Production and characterization of a CD25-specific scFv-Fc antibody secreted from Pichia pastoris. Appl Microbiol Biotechnol 2013, 97:3855-3859. 10.1007/s00253-012-4632-9, 23250227.
36. Kawabe Y. Production of scFv-Fc fusion protein using genetically manipulated quails. J Biosci Bioeng 2006, 102:297-303. 10.1263/jbb.102.297, 17116575.
37. Cao P. Development of a compact anti-BAFF antibody in Escherichia coli. Appl Microbiol Biotechnol 2006, 73:151-157. 10.1007/s00253-006-0432-4, 16625393.
38. Henquet M. Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds. Transgenic Res 2011, 20:1033-1042. 10.1007/s11248-010-9475-5, 3174376, 21188635.
39. Sonoda H. Production of single-chain Fv-Fc fusion protein in stably transformed insect cells. Biochem Eng J 2012, 67:77-82.
40. Klatt S. Production of glycosylated soluble amyloid precursor protein alpha (sAPPalpha) in. J Proteome Res 2013, 12:396-403. 10.1021/pr300693f, 23214446.
41. Thomas AW, Topping AW, Slater JH, Weightman AJ. Localization and functional analysis of structural and regulatory dehalogenase genes carried on DEH from Pseudomonas putida PP3. J Bacteriol 1992, 174:1941-1947. 205800, 1312534.
42. Danquah MK. Growth medium selection and its economic impact on plasmid DNA production. J Biosci Bioeng 2007, 104:490-497. 10.1263/jbb.104.490, 18215636.