Production of single-chain Fv-Fc fusion protein in stably transformed insect cells

Biochemical Engineering Journal

Volumn 67, Issue , 2012, Pages 77-82

  Sonoda, Hiroyuki ^a,b   Kumada, Yoichi ^c   Katsuda, Tomohisa ^a   Yamaji, Hideki ^a  

^a KOBE UNIVERSITY   (Japan)

^b JCR Pharmaceuticals Co   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Fc fusion protein; Insect cell culture; Medium supplement; Recombinant protein production; Single chain variable fragment; Stable expression

Indexed keywords

FUSION PROTEINS; INSECT CELL CULTURE; MEDIUM SUPPLEMENT; RECOMBINANT PROTEIN PRODUCTIONS; STABLE EXPRESSION; VARIABLE FRAGMENT;

CELLS; DIMETHYL SULFOXIDE; GENE ENCODING; GENE EXPRESSION; MAMMALS; ORGANIC SOLVENTS; PRODUCTIVITY; RECOMBINANT PROTEINS; SODIUM;

CELL CULTURE;

DIMETHYL SULFOXIDE; HYBRID PROTEIN; NEOMYCIN; PLASMID DNA; SERICIN; SINGLE CHAIN VARIABLE FRAGMENT IMMUNOGLOBULIN G1 FC FRAGMENT FUSION PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; INSECT CELL; INSECT CELL CULTURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BOVINAE; HEXAPODA; TRICHOPLUSIA NI;

EID: 84862245269     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2012.05.005     Document Type: Article

References (25)

1. Kontermann R.E. Alternative antibody formats. Curr. Opin. Mol. Ther. 2010, 12:176-183.
2. Huston J.S., Levinson D., Mudgett-Hunter M., Tai M.S., Novotný J., Margolies M.N., Ridge R.J., Bruccoleri R.E., Haber E., Crea R., Oppermann H. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 1988, 85:5879-5883.
3. Holliger P., Hudson P.J. Engineered antibody fragments and the rise of single domains. Nat. Biotechnol. 2005, 23:1126-1136.
4. Fernández L.A. Prokaryotic expression of antibodies and affibodies. Curr. Opin. Biotechnol. 2004, 15:364-373.
5. Humphreys D.P. Production of antibodies and antibody fragments in Escherichia coli and a comparison of their functions, uses and modification. Curr. Opin. Drug Discov. Dev. 2003, 6:188-196.
6. Jurado P., Ritz D., Beckwith J., de Lorenzo V., Fernández L.A. Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 2002, 320:1-10.
7. Germain C., Campigna E., Salhi I., Morisseau S., Navarro-Teulon I., Mach J.P., Pèlegrin A., Robert B. Redirecting NK cells mediated tumor cell lysis by a new recombinant bifunctional protein. Protein Eng. Des. Select. 2008, 21:665-672.
8. Heuser C., Guhlke S., Matthies A., Bender H., Barth S., Diehl V., Abken H., Hombach A. Anti-CD30-scFv-Fc-IL-2 antibody-cytokine fusion protein that induces resting NK cells to highly efficient cytolysis of Hodgkin's lymphoma derived tumour cells. Int. J. Cancer 2004, 110:386-394.
9. Sonoda H., Kumada Y., Katsuda T., Yamaji H. Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli. Biochem. Eng. J. 2011, 53:253-259.
10. Senger T., Schädlich L., Gissmann L., Müller M. Enhanced papillomavirus-like particle production in insect cells. Virology 2009, 388:344-353.
11. Yamaji H. Production of antibody in insect cells. Cell Engineering 2011, vol. 7:53-76. Springer Science+Business Media, Dordrecht. M. Al-Rubeai (Ed.).
12. Katakura Y., Kobayashi E., Kurokawa Y., Omasa T., Fujiyama K., Suga K. Cloning of cDNA and characterization of anti-RNase A monoclonal antibody 3A21. J. Ferment. Bioeng. 1996, 82:312-314.
13. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York. third ed.
14. Lu M., Farrell P.J., Johnson R., Iatrou K. A baculovirus (Bombyx mori nuclear polyhedrosis virus) repeat element functions as a powerful constitutive enhancer in transformed insect cells. J. Biol. Chem. 1997, 272:30724-30728.
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
16. Furuta T., Ogawa T., Katsuda T., Fujii I., Yamaji H. Efficient production of an antibody Fab fragment using the baculovirus-insect cell system. J. Biosci. Bioeng. 2010, 110:577-581.
17. Sung Y.H., Lee G.M. Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of bcl-2-overexpressing CHO cells. Biotechnol. Prog. 2005, 21:50-57.
18. Hong J.K., Lee G.M., Yoon S.K. Growth factor withdrawal in combination with sodium butyrate addition extends culture longevity and enhances antibody production in CHO cells. J. Biotechnol. 2011, 155:225-231.
19. Park J.H., Chang K.H., Lee J.M., Lee Y.H., Chung I.S. Optimal production and in vitro activity of recombinant endostatin from stably transformed Drosophila melanogaster S2 cells. In Vitro Cell Dev. Biol. Anim. 2001, 37:5-9.
20. Chang K.H., Park J.H., Lee Y.H., Kim J.H., Chun H.O., Kim J.H., Chung I.S. Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase 2 in stably transformed Drosophila melanogaster S2 cells. Biotechnol. Lett. 2002, 24:1353-1359.
21. Chang K.H., Lee J.M., Jeon H.K., Chung I.S. Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells. Protein Expr. Purif. 2004, 35:69-75.
22. Terada S., Sasaki M., Yanagihara K., Yamada H. Preparation of silk protein sericin as mitogenic factor for better mammalian cell culture. J. Biosci. Bioeng. 2005, 100:667-671.
23. Terada S., Nishimura T., Sasaki M., Yamada H., Miki M. Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma. Cytotechnology 2002, 40:3-12.
24. Takahashi M., Tsujimoto K., Yamada H., Takagi H., Nakamori S. The silk protein, sericin, protects against cell death caused by acute serum deprivation in insect cell culture. Biotechnol. Lett. 2003, 25:1805-1809.
25. Takahashi M., Tsujimoto K., Kato Y., Yamada H., Takagi H., Nakamori S. A sericin-derived peptide protects Sf9 insect cells from death caused by acute serum deprivation. Biotechnol. Lett. 2005, 27:893-897.