A high-performance liquid chromatography assay for Dyrk1a, a down syndrome-associated kinase

Analytical Biochemistry

Volumn 449, Issue 1, 2014, Pages 172-178

  Bui, Linh C ^a   Tabouy, Laure ^a   Busi, Florent ^a   Dupret, Jean Marie ^a   Janel, Nathalie ^a   Planque, Chris ^a   Delabar, Jean Maurice ^a   Rodrigues Lima, Fernando ^a   Dairou, Julien ^a  

^a Groupe Hospitalier Pitié Salpêtrière   (France)

Author keywords

Down syndrome; Dyrk1a; Enzyme assay; HPLC; Kinase

Indexed keywords

AMINO ACIDS; CHROMOSOMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAMMALS; NEURODEGENERATIVE DISEASES; PHOSPHORYLATION;

DOWN SYNDROME; DYRK1A; ENZYME ASSAYS; HPLC; KINASE;

ENZYMES;

BRAIN EXTRACT; DUAL SPECIFICITY TYROSINE PHOSPHORYLATION REGULATED KINASE 1A; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DOWN SYNDROME; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; GENE DOSAGE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

DOWN SYNDROME; DYRK1A; ENZYME ASSAY; HPLC; KINASE;

AMINO ACID SEQUENCE; ANIMALS; BRAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOWN SYNDROME; ENZYME ASSAYS; FLUORESCEIN; FLUORESCENCE; FLUORESCENT DYES; HUMANS; MICE; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; REPRODUCIBILITY OF RESULTS;

EID: 84893399899     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.12.024     Document Type: Article

References (20)

1. W. Becker, and W. Sippl Activation, regulation, and inhibition of DYRK1A FEBS J. 278 2011 246 256
2. J.M. Delabar, D. Theophile, Z. Rahmani, Z. Chettouh, J.L. Blouin, M. Prieur, B. Noel, and P.M. Sinet Molecular mapping of twenty-four features of Down syndrome on chromosome 21 Eur. J. Hum. Genet. 1 1993 114 124
3. J. Guimera, C. Casas, X. Estivill, and M. Pritchard Human minibrain homologue (MNBH/DYRK1): characterization, alternative splicing, differential tissue expression, and overexpression in Down syndrome Genomics 57 1999 407 418 (Pubitemid 29239228)
4. A. Ronan, K. Fagan, L. Christie, J. Conroy, N.J. Nowak, and G. Turner Familial 4.3 Mb duplication of 21q22 sheds new light on the Down syndrome critical region J. Med. Genet. 44 2007 448 451 (Pubitemid 47056871)
5. Y.S. Ryu, S.Y. Park, M.S. Jung, S.H. Yoon, M.Y. Kwen, S.Y. Lee, S.H. Choi, C. Radnaabazar, M.K. Kim, H. Kim, K. Kim, W.J. Song, and S.H. Chung Dyrk1A-mediated phosphorylation of presenilin 1: a functional link between Down syndrome and Alzheimer's disease J. Neurochem. 115 2010 574 584
6. F. Liu, Z. Liang, J. Wegiel, Y.W. Hwang, K. Iqbal, I. Grundke-Iqbal, N. Ramakrishna, and C.X. Gong Overexpression of Dyrk1A contributes to neurofibrillary degeneration in Down syndrome FASEB J. 22 2008 3224 3233
7. I. Ferrer, M. Barrachina, B. Puig, M. Martinez de Lagran, E. Marti, J. Avila, and M. Dierssen Constitutive Dyrk1A is abnormally expressed in Alzheimer disease, Down syndrome, Pick disease, and related transgenic models Neurobiol. Dis. 20 2005 392 400 (Pubitemid 41476265)
8. J. Bain, H. McLauchlan, M. Elliott, and P. Cohen The specificities of protein kinase inhibitors: an update Biochem. J. 371 2003 199 204 (Pubitemid 36458094)
9. T. Adayev, M.C. Chen-Hwang, N. Murakami, J. Wegiel, and Y.W. Hwang Kinetic properties of a MNB/DYRK1A mutant suitable for the elucidation of biochemical pathways Biochemistry 45 2006 12011 12019 (Pubitemid 44497826)
10. J. Bain, L. Plater, M. Elliott, N. Shpiro, C.J. Hastie, H. McLauchlan, I. Klevernic, J.S. Arthur, D.R. Alessi, and P. Cohen The selectivity of protein kinase inhibitors: a further update Biochem. J. 408 2007 297 315
11. W. Xie, N. Ramakrishna, A. Wieraszko, and Y.W. Hwang Promotion of neuronal plasticity by (-)-epigallocatechin-3-gallate Neurochem. Res. 33 2008 776 783 (Pubitemid 351423141)
12. T. Adayev, J. Wegiel, and Y.W. Hwang Harmine is an ATP-competitive inhibitor for dual-specificity tyrosine phosphorylation-regulated kinase 1A (Dyrk1A) Arch. Biochem. Biophys. 507 2011 212 218
13. E. Lilienthal, K. Kolanowski, and W. Becker Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes BMC Biochem. 11 2010 20
14. Y.L. Woods, G. Rena, N. Morrice, A. Barthel, W. Becker, S. Guo, T.G. Unterman, and P. Cohen The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site Biochem. J. 355 2001 597 607 (Pubitemid 32434075)
15. A. Cornish-Bowden Fundamentals of Enzyme Kinetics 2012 Wiley-Blackwell Weinheim, Germany
16. V. Fotaki, M. Dierssen, S. Alcantara, S. Martinez, E. Marti, C. Casas, J. Visa, E. Soriano, X. Estivill, and M.L. Arbones Dyrk1A haploinsufficiency affects viability and causes developmental delay and abnormal brain morphology in mice Mol. Cell Biol. 22 2002 6636 6647
17. F. Guedj, P.L. Pereira, S. Najas, M.J. Barallobre, C. Chabert, B. Souchet, C. Sebrie, C. Verney, Y. Herault, M. Arbones, and J.M. Delabar DYRK1A: a master regulatory protein controlling brain growth Neurobiol. Dis. 46 2012 190 203
18. S. Himpel, P. Panzer, K. Eirmbter, H. Czajkowska, M. Sayed, L.C. Packman, T. Blundell, H. Kentrup, J. Grotzinger, H.G. Joost, and W. Becker Identification of the autophosphorylation sites and characterization of their effects in the protein kinase DYRK1A Biochem. J. 359 2001 497 505 (Pubitemid 33063373)
19. R. Copeland Evaluation of Enzyme Inhibitors in Drug Discovery 2005 John Wiley Hoboken, NJ
20. W.K. Dowjat, T. Adayev, I. Kuchna, K. Nowicki, S. Palminiello, Y.W. Hwang, and J. Wegiel Trisomy-driven overexpression of DYRK1A kinase in the brain of subjects with Down syndrome Neurosci. Lett. 413 2007 77 81 (Pubitemid 46149186)