Development of a sensitive non-radioactive protein kinase assay and its application for detecting DYRK activity in Xenopus laevis oocytes

BMC Biochemistry

Volumn 11, Issue 1, 2010, Pages

  Lilienthal, Eva ^a   Kolanowski, Katharina ^a   Becker, Walter ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ISOENZYME; PHOSPHOPEPTIDE; PHOSPHOSPECIFIC ANTIBODY; PROTEIN DYRK; PROTEIN DYRK1A; PROTEIN DYRK1B; PROTEIN KINASE; UNCLASSIFIED DRUG; ANTIBODY; DYRK KINASE; ISOPROTEIN; PROTEIN SERINE THREONINE KINASE; PROTEIN TYROSINE KINASE; XENOPUS PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; MEASUREMENT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYTE; RADIOMETRY; SENSITIVITY AND SPECIFICITY; XENOPUS LAEVIS; AMINO ACID SEQUENCE; ANIMAL; ENZYMOLOGY; FACTUAL DATABASE; IMMUNOLOGY; IMMUNOPRECIPITATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PHOSPHORYLATION;

EUKARYOTA; MAMMALIA; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; DATABASES, FACTUAL; ENZYME ASSAYS; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOPRECIPITATION; MICE; MOLECULAR SEQUENCE DATA; OOCYTES; PHOSPHORYLATION; PROTEIN ISOFORMS; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; XENOPUS LAEVIS; XENOPUS PROTEINS;

EID: 77956631120     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-11-20     Document Type: Article

References (37)

1. Recent advances in technologies for analyzing protein kinases. A Ishida, I Kameshita, N Sueyoshi, T Takanobu, T Taniguchi, Y Shigeri, J Pharmacol Sci 2007 103 5 11 10.1254/jphs.CP0060026 17202749
2. Current in vitro kinase assays; the quest for a universal format. Y Jia, CM Quinn, S Kwak, RV Talanian, Curr Drug Discov Technol 2008 5 59 69 10.2174/157016308783769414 18537568
3. Rapid protein kinase assay using phosphocellulose-paper absorption. JJ Witt, R Roskoski Jr, Anal Biochem 1975 66 253 258 10.1016/0003-2697(75)90743-5 1147218
4. Isolation of phosphorylated peptides and proteins on ion exchange papers. DB Glass, RA Masaracchia, JR Feramisco, BE Kemp, Anal Biochem 1978 87 566 575 10.1016/0003-2697(78)90707-8 210691
5. Quantitative methods for the analysis of protein phosphorylation in drug development. DM Olive, Expert Rev Proteomics 2004 1 327 341 10.1586/14789450.1. 3.327 15966829
6. A monoclonal antibody to the phosphorylated form of glial fibrillary acidic protein: application to a non-radioactive method for measuring protein kinase activities. T Yano, C Taura, M Shibata, Y Hirono, S Ando, M Kusubata, T Takahashi, M Inagaki, Biochem Biophys Res Commun 1991 175 1144 1151 10.1016/0006-291X(91)91685-6 2025246
7. c-src tyrosine protein kinase activity. K Farley, H Mett, E McGlynn, B Murray, NB Lydon, Anal Biochem 1992 203 151 157 10.1016/0003-2697(92)90056-D 1381873
8. Enzyme-linked immunosorbent assay for the determination of p21-activated kinase activity. JS Yu, SH Chang, WH Chan, HC Chen, J Biochem 2001 29 243 251
9. A chemiluminescent microtiter plate assay for sensitive detection of protein kinase activity. C Lehel, S Daniel-Issakani, M Brasseur, B Strulovici, Anal Biochem 1997 244 340 346 10.1006/abio.1996.9894 9025951
10. A non-radioactive method for the assay of many serine/threonine-specific protein kinases. H Ross, CG Armstrong, P Cohen, Biochem J 2002 366 977 981 12119045
11. Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle. SG Martin, M Berthelot-Grosjean, Nature 2009 459 852 856 10.1038/nature08054 19474792
12. A spatial gradient coordinates cell size and mitotic entry in fission yeast. JB Moseley, A Mayeux, A Paoletti, P Nurse, Nature 2009 459 857 860 10.1038/nature08074 19474789
13. The C. elegans DYRK Kinase MBK-2 Marks Oocyte Proteins for Degradation in Response to Meiotic Maturation. ML Stitzel, J Pellettieri, G Seydoux, Curr Biol 2006 16 56 62 10.1016/j.cub.2005.11.063 16338136
14. Two dual specificity kinases are preferentially induced by wild-type rather than by oncogenic RAS-P21 in Xenopus oocytes. Y Qu, V Adler, T Chu, O Platica, J Michl, S Pestka, L Izotova, M Boutjdir, MR Pincus, Front Biosci 2006 11 2420 2427 10.2741/1979 16720323
15. minibrain: a new protein kinase family involved in postembryonic neurogenesis in Drosophila. F Tejedor, XR Zhu, E Kaltenbach, A Ackermann, A Baumann, I Canal, M Heisenberg, KF Fischbach, O Pongs, Neuron 1995 14 287 301 10.1016/0896-6273(95)90286-4 7857639
16. The MNB/DYRK1A protein kinase: neurobiological functions and Down syndrome implications. B Hämmerle, C Elizalde, J Galceran, W Becker, FJ Tejedor, J Neural Transm Suppl 2003 67 129 137 15068245
17. DYRK1A (dual-specificity tyrosine-phosphorylated and -regulated kinase 1A): a gene with dosage effect during development and neurogenesis. M Dierssen, MM de Lagrán, ScientificWorldJournal 2006 6 1911 1922 10.1100/tsw.2006.319 17205196
18. DYRK1A. W Becker, UCSD-Nature Molecule Pages 2008
19. Role for DYRK family kinases on regulation of apoptosis. K Yoshida, Biochem Pharmacol 2008 76 1389 1394 10.1016/j.bcp.2008.05.021 18599021
20. Mirk/Dyrk1B in cancer. E Friedman, J Cell Biochem 2007 102 274 279 10.1002/jcb.21451 17583556
21. Cooperation to amplify gene-dosage-imbalance effects. S de la Luna, X Estivill, Trends Mol Med 2006 12 451 454 10.1016/j.molmed.2006.08.001 16919501
22. Function and regulation of Dyrk1A: towards understanding Down syndrome. J Park, WJ Song, KC Chung, Cell Mol Life Sci 2009 66 3235 3240 10.1007/s00018-009-0123-2 19685005
23. The kinase DYRK phosphorylates protein-synthesis initiation factor eIF2Bepsilon at Ser539 and the microtubule-associated protein tau at Thr212: potential role for DYRK as a glycogen synthase kinase 3-priming kinase. YL Woods, P Cohen, W Becker, R Jakes, M Goedert, X Wang, CG Proud, Biochem J 2001 355 609 615 11311121
24. DYRK1A-mediated hyperphosphorylation of Tau. A functional link between Down syndrome and Alzheimer disease. SR Ryoo, HK Jeong, C Radnaabazar, JJ Yoo, HJ Cho, HW Lee, IS Kim, YH Cheon, YS Ahn, SH Chung, WJ Song, J Biol Chem 2007 282 34850 34857 10.1074/jbc.M707358200 17906291
25. DYRK1A is a novel negative regulator of cardiomyocyte hypertrophy. C Kuhn, D Frank, R Will, C Jaschinski, R Frauen, HA Katus, N Frey, J Biol Chem 2009 284 17320 17327 10.1074/jbc.M109.006759 19372220
26. Alternative splicing variants of dual specificity tyrosine phosphorylated and regulated kinase 1B exhibit distinct patterns of expression and functional properties. S Leder, H Czajkowska, B Maenz, K De Graaf, A Barthel, HG Joost, W Becker, Biochem J 2003 372 881 888 10.1042/BJ20030182 12633499
27. Enzyme-linked immunosorbent assay for measurement of JNK, ERK, and p38 kinase activities. P Forrer, R Tamaskovic, R Jaussi, Biol Chem 1998 379 1101 1111 10.1515/bchm.1998.379.8-9.1101 9792443
28. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis. E Kinoshita-Kikuta, Y Aoki, E Kinoshita, T Koike, Mol Cell Proteomics 2007 6 356 366 17088264
29. The endogenous and cell cycle-dependent phosphorylation of tau protein in living cells: implications for Alzheimer's disease. S Illenberger, Q Zheng-Fischhöfer, U Preuss, K Stamer, K Baumann, B Trinczek, J Biernat, R Godemann, EM Mandelkow, E Mandelkow, Mol Biol Cell 1998 9 1495 1512 9614189
30. Mnb/Dyrk1A is transiently expressed and asymmetrically segregated in neural progenitor cells at the transition to neurogenic divisions. B Hämmerle, E Vera-Samper, S Speicher, R Arencibia, S Martínez, FJ Tejedor, Dev Biol 2002 246 259 273 10.1006/dbio.2002.0675 12051815
31. A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for endothelin-1 expression. RM Nissen, A Amsterdam, N Hopkins, BMC Dev Biol 2006 6 28 10.1186/1471-213X-6-28 16759393
32. The Yak1 kinase is involved in the initiation and maintenance of hyphal growth in Candida albicans. S Goyard, P Knechtle, M Chauvel, A Mallet, MC Prévost, C Proux, JY Coppée, P Schwartz, F Dromer, H Park, SG Filler, G Janbon, C d'Enfert, Mol Biol Cell 2008 19 2251 2266 10.1091/mbc.E07-09-0960 18321992
33. DYRK1A-dosage imbalance perturbs NRSF/REST levels, deregulating pluripotency and embryonic stem cell fate in Down syndrome. C Canzonetta, C Mulligan, S Deutsch, S Ruf, A O'Doherty, R Lyle, C Borel, N Lin-Marq, F Delom, J Groet, F Schnappauf, S De Vita, S Averill, JV Priestley, JE Martin, J Shipley, G Denyer, CJ Epstein, C Fillat, X Estivill, VL Tybulewicz, EM Fisher, SE Antonarakis, D Nizetic, Am J Hum Genet 2008 83 388 400 10.1016/j.ajhg.2008.08. 012 18771760
34. Specificity determinants of substrate recognition by the protein kinase DYRK1A. S Himpel, W Tegge, R Frank, S Leder, HG Joost, W Becker, J Biol Chem 2000 275 2431 2438 10.1074/jbc.275.4.2431 10644696
35. Up-regulation of sodium pump activity in Xenopus laevis oocytes by expression of heterologous beta 1 subunits of the sodium pump. G Schmalzing, S Gloor, H Omay, S Kröner, H Appelhans, W Schwarz, Biochem J 1991 279 329 336 1719955
36. Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases. W Becker, Y Weber, K Wetzel, K Eirmbter, FJ Tejedor, HG Joost, J Biol Chem 1998 273 25893 25902 10.1074/jbc.273.40.25893 9748265
37. Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation. N Göckler, G Jofre, C Papadopoulos, U Soppa, FJ Tejedor, W Becker, FEBS J 2009 276 6324 6337 10.1111/j.1742-4658.2009.07346.x 19796173