The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

Molecular Breeding

Volumn 33, Issue 2, 2014, Pages 349-362

  Wells, Rachel ^a   Trick, Martin ^a   Soumpourou, Eleni ^a   Clissold, Leah ^a   Morgan, Colin ^a   Werner, Peter ^b   Gibbard, Carl ^b   Clarke, Matthew ^c   Jennaway, Richard ^d   Bancroft, Ian ^a,e  

^a NORWICH RESEARCH PARK   (United Kingdom)

^b 'KWS UK Limited   (United Kingdom)

^c Monsanto UK Ltd   (United Kingdom)

^d Saaten Union UK Ltd   (United Kingdom)

^e UNIVERSITY OF YORK   (United Kingdom)

Author keywords

Brassica; Desaturation; EMS; Fatty acid biosynthesis; Oleic acid; Polyunsaturated fatty acids

Indexed keywords

BIOCHEMISTRY; CROPS; GENES; OILS AND FATS; OILSEEDS; OLEIC ACID; PLANTS (BOTANY);

ARABIDOPSIS THALIANA; BRASSICA; BRASSICA NAPUS; DESATURATION; EMS; FATTY ACID BIOSYNTHESIS; FATTY ACID CONTENTS; GENETIC BASIS; PLANT SPECIES; SEED OIL;

POLYUNSATURATED FATTY ACIDS;

EID: 84893139905     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11032-013-9954-5     Document Type: Article

References (33)

1. Applied Biosystems (2002) BigDye™ Terminator v3. 1 Ready Reaction Cycle Sequencing Kit Protocol.
2. Cheung F, Trick M, Drou N, Lim YP, Park J-Y, Kwon S-J, Kim J-A, Scott R, Pires JC, Paterson AH, Town C, Bancroft I (2009) Comparative analysis between homoeologous genome segments of Brassica napus and its progenitor species reveals extensive sequence-level divergence. Plant Cell Online 21(7): 1912-1928. doi: 10. 1105/tpc. 108. 060376.
3. Dong C, Yu B (2011) Mutation surveyor: an in silico tool for sequencing analysis. Methods Mol Biol 760: 223-237. doi: 10. 1007/978-1-61779-176-5_14.
4. FAO/IAEA (2010) The FAO/IAEA database of mutation enhanced technologies for agriculture (META). http://mvgs. iaea. org/.
5. Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Johnson JE, Odden AR, Comai L, Henikoff S (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164(2): 731-740.
6. Harloff H-J, Lemcke S, Mittasch J, Frolov A, Wu J, Dreyer F, Leckband G, Jung C (2012) A mutation screening platform for rapeseed (Brassica napus L.) and the detection of sinapine biosynthesis mutants. Theor Appl Genet 124(5): 957-969. doi: 10. 1007/s00122-011-1760-z.
7. Harper AL, Trick M, Higgins J, Fraser F, Clissold L, Wells R, Hattori C, Werner P, Bancroft I (2012) Associative transcriptomics of traits in the polyploid crop species Brassica napus. Nat Biotechnol 30(8): 798-802. http://www. nature. com/nbt/journal/v30/n8/abs/nbt. 2302. html#supplementary-information.
8. Hasan M, Seyis F, Badani AG, Pons-Kühnemann J, Friedt W, Lühs W, Snowdon RJ (2006) Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genet Resour Crop Evol 53(4): 793-802. doi: 10. 1007/s10722-004-5541-2.
9. Himelblau E, Gilchrist E, Buono K, Bizzell C, Mentzer L, Vogelzang R, Osborn T, Amasino R, Parkin IP, Haughn G (2009) Forward and reverse genetics of rapid-cycling Brassica oleracea. Theor Appl Genet 118(5): 953-961. doi: 10. 1007/s00122-008-0952-7.
10. James DW, Lim E, Keller J, Plooy I, Ralston E, Dooner HK (1995) Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator. Plant Cell Online 7(3): 309-319. doi: 10. 1105/tpc. 7. 3. 309.
11. Krieg DR (1963) Ethyl methanesulfonate-induced reversion of bacteriophage T4rII mutants. Genetics 48(4): 561-580.
12. Lu G, Moriyama EN (2004) Vector NTI, a balanced all-in-one sequence analysis suite. Brief Bioinform 5(4): 378-388. doi: 10. 1093/bib/5. 4. 378.
13. Marra MA, Kucaba TA, Dietrich NL, Green ED, Brownstein B, Wilson RK, McDonald KM, Hillier LW, McPherson JD, Waterston RH (1997) High throughput fingerprint analysis of large-insert clones. Genome Res 7(11): 1072-1084. doi: 10. 1101/gr. 7. 11. 1072.
14. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeting induced local lesions IN genomes (TILLING) for plant functional genomics. Plant Physiol 123(2): 439-442. doi: 10. 1104/pp. 123. 2. 439.
15. Mei J, Fu Y, Qian L, Xu X, Li J, Qian W (2011) Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a 'virtual allopolyploid' approach. Plant Breed 130(3): 333-337. doi: 10. 1111/j. 1439-0523. 2011. 01850. x.
16. Milne I, Bayer M, Cardle L, Shaw P, Stephen G, Wright F, Marshall D (2010) Tablet-next generation sequence assembly visualisation. Bioinformatics 26(3): 401-402.
17. Miquel M, Browse J (1992) Arabidopsis mutants deficient in polyunsaturated fatty-acid synthesis-biochemical and genetic-characterization. J Biol Chem 267: 1502-1509.
18. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, Browse J (1994) Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell Online 6(1): 147-158. doi: 10. 1105/tpc. 6. 1. 147.
19. Oleykowski C, Bronson Mullins C, Godwin A, Yeung A (1998) Mutation detection using a novel plant endonuclease. Nucleic Acids Res 26(20): 5.
20. O'Neill CM, Bancroft I (2000) Comparative physical mapping of segments of the genome of Brassica oleracea var. albogalabra that are homeologous to sequenced regions of chromosome 4 and 5 of Arabidopsis thaliana. Plant J 23(2): 233-243.
21. Peng Q, Hu Y, Wei R, Zhang Y, Guan C, Ruan Y, Liu C (2010) Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds. Plant Cell Rep 29(4): 317-325. doi: 10. 1007/s00299-010-0823-y.
22. Rana D, Boogaart T, O'Neill CM, Hynes L, Bent E, Macpherson L, Park JY, Lim YP, Bancroft I (2004) Conservation of the microstructure of genome segments in Brassica napus and its diploid relatives. Plant J 40(5): 725-733. doi: 10. 1111/j. 1365-313X. 2004. 02244. x.
23. Scheffler JA, Sharpe AG, Schmidt H, Sperling P, Parkin IAP, Lühs W, Lydiate DJ, Heinz E (1997) Desaturase multigene families of Brassica napus arose through genome duplication. Theor Appl Genet 94: 583-591.
24. Smith MA, Moon H, Chowrira G, Kunst L (2003) Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis. Planta 217: 507-516.
25. Smooker A, Wells R, Morgan C, Beaudoin F, Cho K, Fraser F, Bancroft I (2011) The identification and mapping of candidate genes and QTL involved in the fatty acid desaturation pathway in Brassica napus. Theor Appl Genet 122: 1075-1090.
26. Stemple DL (2004) TILLING-a high-throughput harvest for functional genomics. Nat Rev Genet 5(2): 5. doi: 10. 1038/nrg1273.
27. Stephenson P, Baker D, Girin T, Perez A, Amoah S, King G, Ostergaard L (2010) A rich TILLING resource for studying gene function in Brassica rapa. BMC Plant Biol 10(1): 62.
28. Town CD, Cheung F, Maiti R, Crabtree J, Haas BJ, Wortman JR, Hine EE, Althoff R, Arbogast TS, Tallon LJ, Vigouroux M, Trick M, Bancroft I (2006) Comparative genomics of Brassica oleracea and Arabidopsis thaliana reveal gene loss, fragmentation, and dispersal after polyploidy. Plant Cell 18(6): 1348-1359. doi: 10. 1105/tpc. 106. 041665.
29. Wang N, Wang Y, Tian F, King GJ, Zhang C, Long Y, Shi L, Meng J (2008) A functional genomics resource for Brassica napus: development of an EMS mutagenized population and discovery of FAE1 point mutations by TILLING. New Phytol 180(4): 751-765. doi: 10. 1111/j. 1469-8137. 2008. 02619. x.
30. Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J-H, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires JC, Paterson AH, Chalhoub B, Wang B, Hayward A, Sharpe AG, Park B-S, Weisshaar B, Liu B, Li B, Liu B, Tong C, Song C, Duran C, Peng C, Geng C, Koh C, Lin C, Edwards D, Mu D, Shen D, Soumpourou E, Li F, Fraser F, Conant G, Lassalle G, King GJ, Bonnema G, Tang H, Wang H, Belcram H, Zhou H, Hirakawa H, Abe H, Guo H, Wang H, Jin H, Parkin IAP, Batley J, Kim J-S, Just J, Li J, Xu J, Deng J, Kim JA, Li J, Yu J, Meng J, Wang J, Min J, Poulain J, Hatakeyama K, Wu K, Wang L, Fang L, Trick M, Links MG, Zhao M, Jin M, Ramchiary N, Drou N, Berkman PJ, Cai Q, Huang Q, Li R, Tabata S, Cheng S, Zhang S, Zhang S, Huang S, Sato S, Sun S, Kwon S-J, Choi S-R, Lee T-H, Fan W, Zhao X, Tan X, Xu X, Wang Y, Qiu Y, Yin Y, Li Y, Du Y, Liao Y, Lim Y, Narusaka Y, Wang Y, Wang Z, Li Z, Wang Z, Xiong Z, Zhang Z (2011) The genome of the mesopolyploid crop species Brassica rapa. Nat Genet 43(10): 1035-1039. http://www. nature. com/ng/journal/v43/n10/abs/ng. 919. html#supplementary-information.
31. Yang T-J, Kim JS, Kwon S-J, Lim K-B, Choi B-S, Kim J-A, Jin M, Park JY, Lim M-H, Kim H-I, Lim YP, Kang JJ, Hong J-H, Kim C-B, Bhak J, Bancroft I, Park B-S (2006) Sequence-level analysis of the diploidization process in the triplicated FLOWERING LOCUS C region of Brassica rapa. Plant Cell Online 18(6): 1339-1347. doi: 10. 1105/tpc. 105. 040535.
32. Yang Q, Fan C, Guo Z, Qin J, Wu J, Li Q, Fu T, Zhou Y (2012a) Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents. Theor Appl Genet 125(4): 715-729. doi: 10. 1007/s00122-012-1863-1.
33. Yang Q, Fan C, Guo Z, Qin J, Wu J, Li Q, Fu T, Zhou Y (2012b) Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents. Theor Appl Genet 125(4): 715-729. doi: 10. 1007/s00122-012-1863-1.