Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents

Theoretical and Applied Genetics

Volumn 125, Issue 4, 2012, Pages 715-729

  Yang, Qingyong ^a   Fan, Chuchuan ^a   Guo, Zhenhua ^a   Qin, Jie ^a   Wu, Jianzhong ^a   Li, Qingyuan ^a   Fu, Tingdong ^a   Zhou, Yongming ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CLONE CELLS; CLONING; FATTY ACIDS; MAPPING; PLANTS (BOTANY);

BIOINFORMATIC ANALYSIS; BRASSICA OLERACEA; GENOME STRUCTURES; MOLECULAR CLONING; OILSEED RAPE (BRASSICA NAPUS); QUALITY BREEDING; QUANTITATIVE TRAIT LOCUS; SINGLE NUCLEOTIDE-AMPLIFIED POLYMORPHISMS;

GENES;

BRASSICA NAPUS; BRASSICA OLERACEA; BRASSICA RAPA; CARYA;

EID: 84866310596     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00122-012-1863-1     Document Type: Article

References (52)

1. Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, Somerville CR (1992) Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258: 1353-1355.
2. Barret P, Delourme R, Brunel D, Jourdren C, Horvais R, Renard M (1999) Low linolenic acid level in rapeseed can be easily assessed through the detection of two single base substitution in fad3 genes. In: Proceedings of the 10th international rapeseed congress: new horizons for an old crop. Canberra, Australia. Available at http://www. regional. org. au/au/gcirc/4/385. htm.
3. Broun P, Shanklin J, Whittle E, Somerville C (1998) Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science 282: 1315-1317.
4. Cha RS, Zarbl H, Keohavong P, Thilly WG (1992) Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene. PCR Methods Appl 2: 14-20.
5. Cheng F, Liu S, Wu J, Fang L, Sun S, Liu B, Li P, Hua W, Wang X (2011) BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biol 11: 136.
6. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138: 963-971.
7. Covello PS, Reed DW (1996) Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae. Plant Physiol 111: 223-226.
8. Drenkard E, Richter B, Rozen S, Stutius L, Angell N, Mindrinos M, Cho R, Oefner P, Davis R, Ausubel F (2000) A simple procedure for the analysis of single nucleotide polymorphisms facilitates map-based cloning in Arabidopsis. Plant Physiol 124: 1483-1492.
9. Fan C, Cai G, Qin J, Li Q, Yang M, Wu J, Fu T, Liu K, Zhou Y (2010) Mapping of quantitative trait loci and development of allele-specific markers for seed weight in Brassica napus. Theor Appl Genet 121: 1289-1301.
10. Guo A, Zhu Q, Chen X, Luo J (2007) GSDS: a gene structure display server. Yi Chuan 29: 1023-1026.
11. Henikoff S, Henikoff JG (1993) Performance evaluation of amino acid substitution matrices. Proteins 17: 49-61.
12. Hu J, Quiros C, Arus P, Strass D, Robbelen G (1995) Mapping of a gene determining linolenic acid concentration in rapeseed with DNA-based markers. Theor Appl Genet 90: 258-262.
13. Hu J, Li G, Struss D, Quiros C (1999) SCAR and RAPD markers associated with 18 carbon fatty acids in rapeseed, Brassica napus. Plant Breed 118: 145-150.
14. Hu XY, Sullivan-Gilbert M, Gupta M, Thompson SA (2006) Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theor Appl Genet 113: 497-507.
15. Hu XY, Sullivan-Gilbert M, Gupta M, Thompson SA (2007) G-to-A mutation at a 5′ splice site of fad3c caused impaired splicing in a low linolenic mutant of canola (Brassica napus L.). Plant Biotechnol 24: 397-400.
16. Javidfar F, Ripley VL, Roslinsky V, Zeinali H, Abdmishani C (2006) Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus). Plant Breed 125: 65-71.
17. Jourdren C, Barret P, Brunel D, Delourme R, Renard M (1996a) Specific molecular marker of the genes controlling linolenic acid content in rapeseed. Theor Appl Genet 93: 512-518.
18. Jourdren C, Barret P, Horvais R, Delourme R, Renard M (1996b) Identification of RAPD markers linked to linolenic acid genes in rapeseed. Euphytica 90: 351-357.
19. Kwok S, Kellogg D, McKinney N, Spasic D, Goda L, Levenson C, Sninsky J (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res 18: 999-1005.
20. Kwok S, Chang S-Y, Sninsky JJ, Wang A (1994) A guide to the design and use of mismatched and degenerate primers. PCR Methods Appl 3: S39-S47.
21. Lemieux B, Miquel M, Somerville C, Browse J (1990) Mutants of Arabidopsis with alterations in seed lipid fatty acid composition. Theor Appl Genet 80: 234-240.
22. Li H, Cui X, Arnheim N (1990) Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction. Proc Natl Acad Sci USA 87: 4580-4584.
23. Matthäus B (2006) Utilization of high-oleic rapeseed oil for deep-fat frying of French fries compared to other commonly used edible oils. Eur J Lipid Sci Technol 108: 200-211.
24. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18: 455-457.
25. Mikolajczyk K, Dabert M, Karlowski W, Spasibionek S, Nowakowska J, Cegielska-Taras T, Bartkowiak-Broda I (2010) Allele-specific SNP markers for the new low linolenic mutant genotype of winter oilseed rape. Plant Breed 129: 502-507.
26. Miquel M (1992) Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase. J Biol Chem 267: 1502-1509.
27. Miquel M, James D, Dooner H, Browse J (1993) Arabidopsis requires polyunsaturated lipids for low temperature survival. Proc Natl Acad Sci USA 90: 6208-6212.
28. Morgante M, Salamini F (2003) From plant genomics to breeding practice. Curr Opin Biotechnol 14: 214-219.
29. Newton C, Graham A, Heptinstall L, Powell S, Summers C, Kalsheker N, Smith J, Markham A (1989) Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res 17: 2503-2516.
30. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E (1994) ArabidopsisFAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6: 147-158.
31. Østergaard L, King GJ (2008) Standardized gene nomenclature for the Brassica genus. Plant Methods 4: 10.
32. Pinzi S, Garcia I, Lopez-Gimenez F, Luque de Castro M, Dorado G, Dorado M (2009) The ideal vegetable oil-based biodiesel composition: a review of social, economical and technical implications. Energy Fuels 23: 2325-2341.
33. Rajcan I, Kasha K, Kott L, Beversdorf W (1999) Detection of molecular markers associated with linolenic and erucic acid levels in spring rapeseed (Brassica napus L.). Euphytica 105: 173-181.
34. Rakow G (1973) Selektion auf linol- und linolen-saureghalt in rapssamen nach mutagener behand-lung. Z Planzen 69: 205-209.
35. Röbbelen G, Nitsch A (1975) Genetical and physiological investigations on mutants for polyenoic fatty acids in rapeseed, Brassica napus L. I. Selection and description of new mutants. Z Pflanzenzucht 75: 92-105.
36. Scarth RT, Tang JH (2006) Modification of oil using conventional and transgenic approaches. Crop Sci 46: 1225-1236.
37. Scarth R, McVetty P, Rimmer S, Stefansson B (1988) Stellar low linolenic-high linoleic acid summer rape. Can J Plant Sci 68: 509-511.
38. Scheffler J, Sharpe A, Schmidt H, Sperling P, Parkin I, Lühs W, Lydiate D, Heinz E (1997) Desaturase multigene families of Brassica napus arose through genome duplication. Theor Appl Genet 94: 583-591.
39. Schierholt A, Becker H, Ecke W (2000) Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.). Theor Appl Genet 101: 897-901.
40. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4. 0. Mol Biol Evol 24: 1596-1599.
41. Tanhuanpää P, Schulman A (2002) Mapping of genes affecting linolenic acid content in Brassica rapa ssp. oleifera. Mol Breed 10: 51-62.
42. Tanhuanpää P, Vilkki J, Vilkki H (1995) Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.). Genome 38: 414-416.
43. Tanhuanpää P, Vilkki J, Vilkki H (1996) Mapping of a QTL for oleic acid concentration in spring turnip rape (Brassica rapa ssp. oleifera). Theor Appl Genet 92: 952-956.
44. Tanhuanpää P, Vilkki J, Vihinen M (1998) Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. oleifera). Mol Breed 4: 543-550.
45. Teutonico R, Osborn T (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89: 885-894.
46. Thies W (1971) Schnelle und einfache Analysen der Fettsäurezusammensetzung in einzelnen Rapskotyledonen I. Gaschromatographische und papierchromatographische Methoden. Z Pflanzenzücht 65: 181-202.
47. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876-4882.
48. Thormann C, Romero J, Mantet J, Osborn T (1996) Mapping loci controlling the concentrations of erucic and linolenic acids in seed oil of Brassica napus L. Theor Appl Genet 93: 282-286.
49. Ugozzoli L, Wallace RB (1992) Application of an allele-specific polymerase chain reaction to the direct determination of ABO blood group genotypes. Genomics 12: 670-674.
50. Uzunova M, Ecke W, Weissleder K, Röbbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90: 194-204.
51. Warner K, Knowlton S (1997) Frying quality and oxidative stability of high-oleic corn oils. J Am Oil Chem Soc 74: 1317-1322.
52. Wittkop B, Snowdon R, Friedt W (2009) Status and perspectives of breeding for enhanced yield and quality of oilseed crops for Europe. Euphytica 170: 131-140.