Rapid detection of Vibrio parahaemolyticus in raw oysters using immunomagnetic separation combined with loop-mediated isothermal amplification

International Journal of Food Microbiology

Volumn 174, Issue , 2014, Pages 123-128

  Zeng, Jing ^a   Wei, Haiyan ^a   Zhang, Lei ^a,c   Liu, Xuefeng ^b   Zhang, Haiyu ^c   Cheng, Jinxia ^a,c   Ma, Dan ^a   Zhang, Ximeng ^a   Fu, Pubo ^a   Liu, Li ^a  

^a Beijing Entry Exit Inspection and Quarantine Bureau   (China)

^b NATIONAL CENTER FOR NANOSCIENCE AND TECHNOLOGY   (China)

^c BEIJING UNIVERSITY OF AGRICULTURE   (China)

Author keywords

Immunomagnetic separation; Loop mediated isothermal amplification (LAMP); Nanoparticle; Oyster; Vibrio parahaemolyticus

Indexed keywords

MAGNETIC NANOPARTICLE; MONOCLONAL ANTIBODY;

ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL SUSPENSION; COLONY FORMING UNIT; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; IMMUNOMAGNETIC SEPARATION; INCUBATION TIME; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; OYSTER; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIBRIO PARAHAEMOLYTICUS;

IMMUNOMAGNETIC SEPARATION; LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP); NANOPARTICLE; OYSTER; VIBRIO PARAHAEMOLYTICUS;

ANIMALS; BACTERIAL PROTEINS; FOOD MICROBIOLOGY; IMMUNOMAGNETIC SEPARATION; LIMIT OF DETECTION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OSTREIDAE; TIME FACTORS; VIBRIO PARAHAEMOLYTICUS;

EID: 84893094697     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2014.01.004     Document Type: Article

References (29)

1. Campbell M.S., Wright A.C. Real-time PCR analysis of Vibrio vulnificus from oysters. Appl. Environ. Microbiol. 2003, 69:7137-7144.
2. Datta S., Janes M.E., Simonson J.G. Immunomagnetic separation and coagglutination of Vibrio parahaemolyticus with anti-flagellar protein monoclonal antibody. Clin. Vaccine Immunol. 2008, 15:1541-1546.
3. Falcioni T., Papa S., Campana R., Manti A., Battistelli M., Baffone W. State transitions of Vibrio parahaemolyticus VBNC cells evaluated by flow cytometry. Cytometry B Clin. Cytom. 2008, 74:272-281.
4. Fedio W.M., Jinneman K.C., Yoshitomi K.J., Zapata R., Wendakoon C.N., Browning P., Weagant S.D. Detection of E. coli O157:H7 in raw ground beef by Pathatrix™ immunomagnetic-separation, real-time PCR and cultural methods. Int. J. Food Microbiol. 2011, 148:87-92.
5. Gehring A.G., Albin D.M., Irwin P.L., Reed S.A., Tu S.I. Comparison of enzyme-linked immunomagnetic chemiluminescence with U.S. Food and Drug Administration's Bacteriological Analytical Manual method for the detection of Escherichia coli O157:H7. J. Microbiol. Methods 2006, 67:527-533.
6. Jacobsen C.S. Microscale detection of specific bacterial DNA in soil with a magnetic capture-hybridization and PCR amplification assay. Appl. Environ. Microbiol. 1995, 61:3347-3352.
7. Jadeja R., Janes M.E., Simonson J.G. Immunomagnetic separation of Vibrio vulnificus with antiflagellar monoclonal antibody. J. Food Prot. 2010, 73:1288-1293.
8. Jeníková G., Pazlarová J., Demnerová K. Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay. Int. Microbiol. 2000, 3:225-229.
9. Kanayeva D.A., Wang R., Rhoads D., Erf G.F., Slavik M.F., Tung S., Li Y. Efficient separation and sensitive detection of Listeria monocytogenes using an impedance immunosensor based on magnetic nanoparticles, a microfluidic chip, and an interdigitated microelectrode. J. Food Prot. 2012, 75:1951-1959.
10. Liberti P.A., Chiarappa J.N., Hovsepian A.C., Rao C.G. Bioreceptor ferrofluids: novel characteristics and their utility in medical applications. Fine Particles Science and Technology 1996, 777-790. Kluwer Academic Publishers. E. Pelizzetti (Ed.).
11. Liu X., Zhang L., Zeng J., Gao Y., Tang Z. Superparamagnetic nano-immunobeads toward food safety insurance. J. Nanoparticle Res. 2013, 15:1796-1805.
12. Nemoto J., Sugawara C., Akahane K., Hashimoto K., Kojima T., Ikedo M., Konuma H., Hara-Kudo Y. Rapid and specific detection of the thermostable direct hemolysin gene in Vibrio parahaemolyticus by loop-mediated isothermal amplification. J. Food Prot. 2009, 72:748-754.
13. Nordstrom J.L., Vickery M.C., Blackstone G.M., Murray S.L., DePaola A. Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters. Appl. Environ. Microbiol. 2007, 73:5840-5847.
14. Ochoa M.L., Harrington P.B. Immunomagnetic isolation of enterohemorrhagic Escherichia coli O157:H7 from ground beef and identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches. Anal. Chem. 2005, 77:5258-5267.
15. Okura M., Osawa R., Iguchi A., Arakawa E., Terajima J., Watanabe H. Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group-specific multiplex PCR assay. J. Clin. Microbiol. 2003, 41:4676-4682.
16. Potasman I., Paz A., Odeh M. Infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective. Clin. Infect. Dis. 2002, 35:921-928.
17. Pyle B.H., Broadaway S.C., McFeters G.A. Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 1999, 65:1966-1972.
18. Rijpens N., Herman L., Vereecken F., Jannes G., De Smedt J., De Zutter L. Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 1999, 46:37-44.
19. Seo S.M., Cho I.H., Jeon J.W., Cho H.K., Oh E.G., Yu H.S., Shin S.B., Lee H.J., Paek S.H. An ELISA-on-a-chip biosensor system coupled with immunomagnetic separation for the detection of Vibrio parahaemolyticus within a single working day. J. Food Prot. 2010, 73:1466-1473.
20. Shim W.B., Choi J.G., Kim J.Y., Yang Z.Y., Lee K.H., Kim M.G., Ha S.D., Kim K.S., Kim K.Y., Kim C.H., Eremin S.A., Chung D.H. Enhanced rapidity for qualitative detection of Listeria monocytogenes using an enzyme-linked immunosorbent assay and immunochromatography strip test combined with immunomagnetic bead separation. J. Food Prot. 2008, 71:781-789.
21. Su Y.C., Liu C. Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol. 2007, 24:549-558.
22. Tomoyasu T. Improvement of the immunomagnetic separation method selective for Escherichia coli O157 strains. Appl. Environ. Microbiol. 1998, 64:376-382.
23. Varshney M., Yang L., Su X.L., Li Y. Magnetic nanoparticle-antibody conjugates for the separation of Escherichia coli O157:H7 in ground beef. J. Food Prot. 2005, 68:1804-1811.
24. Yamazaki W., Kumeda Y., Misawa N., Nakaguchi Y., Nishibuchi M. Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of Vibrio parahaemolyticus and related Vibrio species. Appl. Environ. Microbiol. 2010, 76:820-828.
25. Yang H., Qu L., Wimbrow A.N., Jiang X., Sun Y. Rapid detection of Listeria monocytogenes by nanoparticle-based immunomagnetic separation and real-time PCR. Int. J. Food Microbiol. 2007, 118:132-138.
26. Yang Y., Xu F., Xu H., Aguilar Z.P., Niu R., Yuan Y., Sun J., You X., Lai W., Xiong Y., Wan C., Wei H. Magnetic nano-beads based separation combined with propidium monoazide treatment and multiplex PCR assay for simultaneous detection of viable Salmonella typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in food products. Food Microbiol. 2013, 34:418-424.
27. Yoshitomi K.J., Jinneman K.C., Zapata R., Weagant S.D., Fedio W.M. Detection and isolation of low levels of E. coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment. J. Food Sci. 2012, 77:M481-M489.
28. Zhu P., Shelton D.R., Li S., Adams D.L., Karns J.S., Amstutz P., Tang C.M. Detection of E. coli O157:H7 by immunomagnetic separation coupled with fluorescence immunoassay. Biosens. Bioelectron. 2011, 30:337-341.
29. Zhu R.G., Li T.P., Jia Y.F., Song L.F. Quantitative study of viable Vibrio parahaemolyticus cells in raw seafood using propidium monoazide in combination with quantitative PCR. J. Microbiol. Methods 2012, 90:262-266.