Understanding the relevance of local conformational stability and dynamics to the aggregation propensity of an IgG1 and IgG2 monoclonal antibodies

Protein Science

Volumn 22, Issue 10, 2013, Pages 1295-1305

  Thakkar, Santosh V ^a   Sahni, Neha ^a   Joshi, Sangeeta B ^a   Kerwin, Bruce A ^b   He, Feng ^b   Volkin, David B ^a   Middaugh, C Russell ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b AMGEN INC   (United States)

Author keywords

Aggregation; Circular dichroism; Conformational stability; Differential scanning calorimetry; Dynamics; Fluorescence spectroscopy; Light scattering; Monoclonal antibody

Indexed keywords

IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY;

ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL STABILITY; DIFFERENTIAL SCANNING CALORIMETRY; EXPOSURE; FLUORESCENCE ANALYSIS; LIGHT SCATTERING; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; STEADY STATE; TEMPERATURE DEPENDENCE;

AGGREGATION; CIRCULAR DICHROISM; CONFORMATIONAL STABILITY; DIFFERENTIAL SCANNING CALORIMETRY; DYNAMICS; FLUORESCENCE SPECTROSCOPY; LIGHT SCATTERING; MONOCLONAL ANTIBODY;

ACRYLAMIDE; ANTIBODIES, MONOCLONAL; CIRCULAR DICHROISM; FLUORESCENCE; IMMUNOGLOBULIN G; KINETICS; MULTIPROTEIN COMPLEXES; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; TEMPERATURE; TRYPTOPHAN;

EID: 84892936418     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2316     Document Type: Article

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