Effect of buffer species on the unfolding and the aggregation of humanized IgG

Journal of Biochemistry

Volumn 142, Issue 3, 2007, Pages 383-391

  Kameoka, Daisuke ^a,b   Masuzaki, Etsuro ^a,b   Ueda, Tadashi ^a   Imoto, Taiji ^b,c  

^a KYUSHU UNIVERSITY   (Japan)

^b CHUGAI PHARMACEUTICAL CO LTD   (Japan)

^c SOJO UNIVERSITY   (Japan)

Author keywords

Buffer; DSC; Humanized antibody; Protein aggregation; Protein stabilization

Indexed keywords

2 (N MORPHOLINO) ETHANE SULFONATE; 3 (N MORPHOLINO) PROPANE SULFONATE; ACETIC ACID; BUFFER; CITRIC ACID; IMIDAZOLE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; PHOSPHATE; RECOMBINANT ANTIBODY; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; DIFFERENTIAL SCANNING CALORIMETRY; LIGHT SCATTERING; MOLECULAR DYNAMICS; PH MEASUREMENT; PROTEIN AGGREGATION; PROTEIN STABILITY; PROTEOMICS;

BUFFERS; CALORIMETRY, DIFFERENTIAL SCANNING; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEAT; HUMANS; IMMUNOGLOBULIN G; PROTEIN DENATURATION; TEMPERATURE;

EID: 36849028552     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvm145     Document Type: Article

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