Production, purification and characterization of a novel GH 12 family endoglucanase from aspergillus terreus and its application in enzymatic degradation of delignified rice straw

International Biodeterioration and Biodegradation

Volumn 88, Issue , 2014, Pages 150-161

  Narra, Madhuri ^a   Dixit, Garima ^a   Divecha, Jyoti ^b   Kumar, Kiran ^a   Madamwar, Datta ^b   Shah, Amita R ^b  

^a Sardar Patel Renewable Energy Research Institute   (India)

^b SARDAR PATEL UNIVERSITY   (India)

Author keywords

Aspergillus terreus; Endoglucanase; Enzymatic hydrolysis; Gel filtration; Kinetics; Purification

Indexed keywords

ASPERGILLUS TERREUS; ENDOGLUCANASES; GEL FILTRATION; GEL-FILTRATION CHROMATOGRAPHY; LIGNOCELLULOSIC BIOMASS; PEPTIDE MASS FINGERPRINTING; RESPONSE SURFACE METHODOLOGY; SOLID-STATE FERMENTATION;

ASPERGILLUS; ENZYMATIC HYDROLYSIS; ENZYME KINETICS; GEL PERMEATION CHROMATOGRAPHY; PURIFICATION; SACCHARIFICATION;

ENZYME ACTIVITY;

BIODEGRADATION; BIOTECHNOLOGY; CELLULOSE; ENZYME ACTIVITY; FERMENTATION; FUNGUS; HYDROLYSIS; LIGNIN; PURIFICATION; REACTION KINETICS; RICE; STRAW;

EID: 84892637724     PISSN: 09648305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ibiod.2013.12.016     Document Type: Article

References (46)

1. Akiba S., Kimura Y., Yamamoto H., Kumagai H. Purification and characterization of a protease resistant cellulase from Aspergillus niger. J.Ferment. Bioeng. 1995, 79:125-130.
2. Andrews P. Estimation of molecular weights of proteins by sephadex gel filtration. Biochem. J. 1964, 9:222-223.
3. Anuradha J. Response surface methodology for the evaluation and comparison of cellulase production by Aspergillus nidulans SU04 and Aspergillus nidulans MTCC344 cultivated on pretreated sugarcane bagasse. Chem. Biochem. Eng. J. 2011, 25:501-511.
4. Barakat A., Monlau F., Steyer J.P., Carrerre H. Effect of lignin-derived and furan compounds found in lignocellulosic hydrolysates on biomethane production. Bioresour. Technol. 2012, 104:90-99.
5. Box G.E.P., Wilson K.B. On the experimental attainment of optimum conditions. J.R. Stat. Soc. Ser. B. Meth. 1951, 13:1-45.
6. Bhat M.K. Cellulases and related enzymes in biotechnology. Biotechnol. Adv. 2000, 18:355-383.
7. Bush D.S., Sticher L., Van Huystee R., Wagner D., Jones R.L. The calcium requirement for stability and enzymatic activity of two isoforms of barely aleurone α-amylase. J.Biolog. Chem. 1989, 64:19392-19398.
8. Chen G., Du J., Zhuang L., Gao P. Purification and properties of endoglucanases from Aspergillus aculeatus SM-L22. Wei Sheng Wu Xue Bao 2001, 41:469-474.
9. Dongyang L., Ruifu Z., Xingming Y., Hongsheng W., Dabing X., Zhu T., Qirong S. Thermostable cellulase production of Aspergillus fumigates Z5 under solid state fermentation and its application in degradation of agricultural wastes. Int. Biodeterior. Biodegrad. 2011, 65:717-725.
10. Elshafei A.M., Hassan M.W., Haroun B.M., Abdel-Fatah O.M., Atta H.M., Othman A.M. Purification and properties of an endoglucanase of Aspergillus terreus DSM 826. J.Basic Microbiol. 2009, 49:426-432.
11. Erica T.P., Ivana Stankovic., Rodrigo L.S., Marcelo V.L., Flavio H.S., Nei P.J., Igor P., Munir S.S. X-ray structure and molecular dynamics simulations of endoglucanase 3 from Trichoderma harnianum: structural organization and substrate recognition by endoglucanases that lack cellulose binding module. PLOS One 2013, 8:e59069.
12. Gao J.M., Weng H.B., Zhu D.H., Yuan M.X., Guan F.X., Xi Y. Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid state cultivation of corn stover. Bioresour. Technol. 2008, 99:7623-7629.
13. Ghose T. Measurement of cellulase activities. Commission on Biotechnology 1994.
14. Harris P.V., Welner D., McFarland K.C., Re E., Navarro Poulsen J.C., Brown K., Salbo R., Ding H., Vlansenko E., Merino S., Xu F., Cherry J., Larsen S., Lo Leggio L. Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolyse family 61: structure and function of a large, enigmatic family. Biochemistry 2010, 49:3305-3316.
15. Heyda J., Pokorna J., Vrbka L., Vacha R., Jagoda-Cwiklik B., Konvalinka J., Jungwirth P., Vondrasek J. Phys. Chem. Chem. Phys. 2009, 11:7599.
16. Horn S.J., Vaaje-Kolstad G., Westereng B., Eijsink V.G. Novel enzymes for the degradation of cellulose. Biotechnol. Biofuels 2012, 5:45.
17. Immanuel G., Akila-Bhagavath C.M., Iyappa-Raj P., Esakkiraj P., Palavesam A. Production and partial purification of cellulase by Aspergillus niger and Aspergillus fumigatus fermented in coir waste and saw dust. Internet J. Microbiol. 2007, 3:1-20.
18. Inouhe M., Hayashi K., Nevins D.J. Polypeptide characteristics and immunological properties of exo-and endoglucanases purified from maize coleoptiles cell walls. J.Plant Physiol. 1999, 154:334-340.
19. Iqbal H.M.N., Ahmed I., Zia M.A., Irfan M. Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viridae under SSF and its detergent compatibility. Adv. Biosci. Biotechnol. 2011, 2:149-156.
20. Kumari A., Singh V.K., Fitter J., Polen T., Kayastha A.M. α-amylase from germinating soyabean (Glycine max) seeds-purification, characterization and sequential similarity of conserved and catalytic aminoacid residues. Phytochemistry 2010, 7:1657-1666.
21. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
22. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J.Biolog. Chem. 1951, 193:265-275.
23. Mandels M., Weber J. The production of cellulases. Adv. Chem. Ser. 1969, 95:391-414.
24. Marimuthu J., Ah-Reum J., Kyoung-Mi L., Won- S., Deok-Kun O., Yeong-Suk K., In-Won K., Jung-Kul L. Characterization of endo-β-1,4-glucanase from a novel strain of Penicillium pinophilum KMJ601. Appl. Microbiol. Biotechnol. 2010, 85:1005-1014.
25. Miettinen-Oinonen A., Londesborough J., Joutsjoki V., Lantto R., Ehmaanpera J. Three cellulases from Melanocarpus albomyces for textile treatment at neural pH. Enzyme Microb. Technol. 2004, 34:332-341. Primalco Ltd. Biotec.
26. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 1959, 31:426-428.
27. Muhammad R.J., Muhammad H.R., Habibullah N., Muhammad R., Raheela P. Catalytic and thermodynamic characterization of endoglucanase (CMCase) from Aspergillus oryzae cmc-1. Appl. Biochem. Biotechnol. 2009, 157:483-497.
28. Narra M., Dixit G., Divecha J., Datta M., Shah A.R. Production of cellulases by solid state fermentation with Aspergillus terreus and enzymatic hydrolysis of mild-alkali treated rice straw. Bioresour. Technol. 2012, 121:355-361.
29. Nazir A., Soni H.S., Saini R.K., Manhas R.K., Chandha B.S. Purification and characterization of an endoglucanase from Aspergillus terreus highly active against barely β-glucan and xyloglucan. World J. Microbiol. Biotechnol. 2009, 25:1189-1197.
30. Panagiotou G., Kekos D., Macris B.J., Christakopoulos P. Production of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation. Ind. Crops Prod. 2003, 18:37-45.
31. Pandey A., Ashakumary L., Selvakumar P., Vijyalakshmi K.S. Influence of water activity on growth and activity of Aspergillus niger for glycoamylase production in solid state fermentation. World J. Microbiol. Biotechnol. 1994, 10:485-486.
32. Parry N.J., Beever D.E., Owen E., Nerincks W., Claeyssens M., Beeumen J.V. Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus. Arch. Biochem. Biophys. 2002, 404:243-253.
33. Ragothaman M.Y., Andrew J.R., Jeffrey D.W., Tazez Z.S. Thermostability in endoglucanase is fold specific. BMC Struct. Biol. 2011, 11:10.
34. Rahman Z., Shida Y., Furukawa T., Suzuki Y., Okada H., Ogasawara W., Morikawa Y. Application of Trichoderma reesei cellulase and xylanase promoters through homologous recombination for enhanced production of extracellular beta-glucosidase I. Biosci. Biotechnol. Biochem. 2009, 73:1083-1089.
35. Rye C.S., Withers S.G. Glycosidase mechanism. Curr. Opin. Chem. Biol. 2000, 4:573-580.
36. Soni R., Nazir A., Chadha B.S., Saini H.S. Novel sources of fungal cellulases for efficient deinking of composite paper waste. Bioresources 2008, 3:234-246.
37. Saha S., Roy R., Sen S.K., Ray A.K. Characterization of cellulase producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp. Ctenopharyngodon idella (Valenciennes). Aquac. Res. 2006, 37:380-388.
38. Sakon J., Irwin D., Karplus P.A. Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nat. Struct. Biol. 1997, 4:810-818.
39. Sandgren M., Stanlberg J., Mitchinson C. Structural and biochemical studies of GH family 12 cellulases: improved thermal stability and ligand complexes. Prog. Biophys. Mol. Biol. 2005, 89:246-291.
40. Sarria-Alfonso V., Sierra J.S., Morales M.A., Rojas I.G., Sarmiento N.M., Pinales P. Culture media statistical optimization for biomass production of lignolytic fungus for future rice straw degradation. Indian J. Microbiol. 2013, 10.1007/s12088-013-0358-3.
41. Sujit S.J., Saurabh S.D., Tae-SU K., In-Won K., Jung-Kul L. Characterization of a novel endo-β-1,4-glucanase from Armillaria gemina and its application in biomass hydrolysis. Appl. Microbiol. Biotechnol. 2013, 10.1007/s00253-013-4894-x.
42. Tao Y.M., Zhu X.Z., Huang J.Z., Ma S.J., Wu X.B., Long M.N., Chen Q.X. Purification and properties of endoglucanase from a sugarcane bagasse hydrolyzing strain, Aspergillus glaucus XC9. J.Agric. Food Chem. 2010, 58:6126-6130.
43. Thi H.P., Dinh T.Q., Ngoc M.N. Optimization of endoglucanase production by Aspergillus niger VTCC-F021. Aust. J. Basic Appl. Sci. 2010, 4:4151-4157.
44. van Solingen P., et al. Cloning and expression of an endoglucanase gene from a novel Streptomyces isolated from an East Africa soda lake. Extremophiles 2001, 5:333-341.
45. Watson B.J., Zhang H., Longmire A.G., Moon Y.H., Hutcheson S.W. Processive endoglucanase mediate degradation of cellulose by Saccharophagus degradans. J.Bacteriol. 2009, 191:5697-5705.
46. Wood T.M. Fungal cellulases. Biochem. Soc. Trans. 1992, 20:45-53.