Expression and biological activity of the cystine knot bioinsecticide PA1b (Pea Albumin 1 subunit b)

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Eyraud, Vanessa ^a   Karaki, Lamis ^a   Rahioui, Isabelle ^a   Sivignon, Catherine ^a   Da Silva, Pedro ^a   Rahbé, Yvan ^a   Royer, Corinne ^a   Gressent, Frédéric ^a  

^a UNIVERSITÉ DE LYON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; CYSTEINE; CYSTINE; INSECTICIDE; ISOPROTEIN; KNOTTIN; PEA ALBUMIN 1 SUBUNIT A PEPTIDE; PEA ALBUMIN 1 SUBUNIT B PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; CYSTINE KNOT MOTIF; GENE CASSETTE; GENE CONSTRUCT; INSECTICIDAL ACTIVITY; MOLECULE; NICOTIANA BENTHAMIANA; NONHUMAN; PA1 GENE; PEA; PEPTIDE SYNTHESIS; PLANT GENE; PLANT LEAF; PLANT SEED; PROTEIN EXPRESSION; PROTEIN PROCESSING; SOIL INFILTRATION;

AMINO ACID SEQUENCE; BIOLOGICAL CONTROL AGENTS; DNA, COMPLEMENTARY; GENE EXPRESSION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; INSECTICIDES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEAS; PLANT PROTEINS; PROTEIN ISOFORMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; TOBACCO; TOXINS, BIOLOGICAL;

EID: 84892591176     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081619     Document Type: Article

References (29)

1. Delobel B, Grenier AM, Gueguen J, Ferrasson E, Mbaiguinam M (1998) Utilisation d'un polypeptide dérivéd'une albumine PA1b de légumineuse comme insecticide. French Patent 98 05877.
2. Gressent F, Da Silva P, Eyraud V, Karaki L, Royer C (2011) Pea Albumin 1 subunit b (PA1b), a promising bioinsecticide of plant origin. Toxins (Basel) 3: 1502-1517.
3. Higgins TJ, Chandler PM, Randall PJ, Spencer D, Beach LR, et al. (1986) Gene structure, protein structure, and regulation of the synthesis of a sulfur-rich protein in pea seeds. J Biol Chem 261: 11124-11130.
4. Jouvensal L, Quillien L, Ferrasson E, Rahbe Y, Gueguen J, et al. (2003) PA1b, an insecticidal protein extracted from pea seeds (Pisum sativum): 1H-2-D NMR study and molecular modeling. Biochemistry 42: 11915-11923.
5. Craik DJ (2012) Host-defense activities of cyclotides. Toxins (Basel) 4: 139-156.
6. Craik DJ, Swedberg JE, Mylne JS, Cemazar M (2012) Cyclotides as a basis for drug design. Expert Opin Drug Discov 7: 179-194.
7. Kolmar H (2009) Biological diversity and therapeutic potential of natural and engineered cystine knot miniproteins. Curr Opin Pharmacol 9: 608-614.
8. Da Silva P, Strzepa A, Jouvensal L, Rahioui I, Gressent F, et al. (2009) A folded and functional synthetic PA1b: an interlocked entomotoxic miniprotein. Biopolymers 92: 436-444.
9. Da Silva P, Rahioui I, Laugier C, Jouvensal L, Meudal H, et al. (2010) Molecular requirements for the insecticidal activity of the plant peptide pea albumin 1 subunit b (PA1b). J Biol Chem 285: 32689-32694.
10. Louis S, Delobel B, Gressent F, Duport G, Diol O, et al. (2007) Broad screening of the legume family for variability in seed insecticidal activities and for the occurrence of the A1b-like knottin peptide entomotoxins. Phytochemistry 68: 521-535.
11. Gillon AD, Saska I, Jennings CV, Guarino RF, Craik DJ, et al. (2008) Biosynthesis of circular proteins in plants. Plant J 53: 505-515.
12. Louis S, Delobel B, Gressent F, Rahioui I, Quillien L, et al. (2004) Molecular and biological screening for insect-toxic seed albumins from four legume species. Plant Science 167: 705-714.
13. Chen Q, He J, Phoolcharoen W, Mason HS (2011) Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants. Hum Vaccin 7: 331-338.
14. Wydro M, Kozubek E, Lehmann P (2006) Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. Acta Biochim Pol 53: 289-298.
15. Poth AG, Colgrave ML, Lyons RE, Daly NL, Craik DJ (2011) Discovery of an unusual biosynthetic origin for circular proteins in legumes. Proc Natl Acad Sci U S A 108: 10127-10132.
16. Gressent F, Duport G, Rahioui I, Pauchet Y, Bolland P, et al. (2007) Biological activity and binding site characteristics of the PA1b entomotoxin on insects from different orders. Journal of Insect Science 7: -.
17. Conlan BF, Gillon AD, Craik DJ, Anderson MA (2010) Circular proteins and mechanisms of cyclization. Biopolymers 94: 573-583.
18. Eliasen R, Daly NL, Wulff BS, Andresen TL, Conde-Frieboes KW, et al. (2012) Design, synthesis, structural and functional characterization of novel melanocortin agonists based on the cyclotide kalata B1. J Biol Chem 287: 40493-40501.
19. Saska I, Gillon AD, Hatsugai N, Dietzgen RG, Hara-Nishimura I, et al. (2007) An asparaginyl endopeptidase mediates in vivo protein backbone cyclization. The journal of biological chemstry 282: 29721-29728.
20. Conlan BF, Colgrave ML, Gillon AD, Guarino R, Craik DJ, et al. (2012) Insights into processing and cyclization events associated with biosynthesis of the cyclic Peptide kalata B1. J Biol Chem 287: 28037-28046.
21. Reinwarth M, Glotzbach B, Tomaszowski M, Fabritz S, Avrutina O, et al. (2013) Oxidative folding of peptides with cystine-knot architectures: kinetic studies and optimization of folding conditions. Chembiochem 14: 137-146.
22. Gressent F, Rahioui I, Rahbe Y (2003) Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus. Eur J Biochem 270: 2429-2435.
23. Da Silva P, Eyraud V, Carre-Pierrat M, Sivignon C, Rahioui I, et al. (2012) High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl- medicagenate, from Medicago truncatula seeds, for Sitophilus oryzae. BMC Chem Biol 12: 3.
24. Curtis MD, Grossniklaus U (2003) A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133: 462-469.
25. Voinnet O, Rivas S, Mestre P, Baulcombe D (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33: 949-956.
26. Batoko H, Zheng HQ, Hawes C, Moore I (2000) A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants. Plant Cell 12: 2201-2218.
27. Sparkes IA, Runions J, Kearns A, Hawes C (2006) Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat Protoc 1: 2019-2025.
28. Perrin S, Gilliland G (1990) Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer. Nucleic Acids Res 18: 7433-7438.
29. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, et al. (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 7: 539.