Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry

Proteomics

Volumn 14, Issue 1, 2014, Pages 19-23

  Luo, Lin ^a   King, Nathan P ^a   Yeo, Jeremy C ^a   Jones, Alun ^a   Stow, Jennifer L ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

GST pull down; LC MS MS; PreScission protease; Protease cleavage elution; Technology

Indexed keywords

BINDING PROTEIN; GLUTATHIONE TRANSFERASE; GUANOSINE TRIPHOSPHATASE; PROTEIN; PROTEIN RAB31; PROTEINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELUTION; IMMUNOBLOTTING; MASS SPECTROMETRY; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROCEDURES; PROTEASE CLEAVAGE ELUTION; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN PROTEIN INTERACTION; SENSITIVITY ANALYSIS;

GST PULL-DOWN; LC-MS/MS; PRESCISSION PROTEASE; PROTEASE CLEAVAGE ELUTION; TECHNOLOGY;

ANIMALS; BIOTECHNOLOGY; HUMANS; MASS SPECTROMETRY; MICE; PROTEOMICS; RAB GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 84892405408     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201300315     Document Type: Article

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