On-bead tryptic proteolysis: An attractive procedure for LC-MS/MS analysis of the Drosophila caspase 8 protein complex during immune response against bacteria

Journal of Proteomics

Volumn 75, Issue 15, 2012, Pages 4610-4619

  Fukuyama, Hidehiro ^a   Ndiaye, Sega ^b   Hoffmann, Jules ^a   Rossier, Jean ^a   Liuu, Sophie ^b   Vinh, Joëlle ^b   Verdier, Yann ^b  

^a CNRS   (France)

^b ESPCI ParisTech   (France)

Author keywords

Dredd; Imd pathway; On bead digestion; Protein complex; Technology

Indexed keywords

BG4 PROTEIN; BIOTIN; CASPASE 8; COMPLEMENTARY DNA; CP190 PROTEIN; DREDD PROTEIN; DROSOPHILA PROTEIN; HEAT SHOCK PROTEIN 7C; PLASMID DNA; Q9VP57 PROTEIN; Q9VXQ5 PROTEIN; Q9W1B9 PROTEIN; STREPTAVIDIN; TBB1 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL INFECTION; DROSOPHILA; EXPRESSION VECTOR; GENETIC TRANSFECTION; IMMUNE RESPONSE; INSECT CELL CULTURE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; ON BEAD DIGESTION; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN TARGETING;

ANIMALS; BACTERIA; BACTERIAL INFECTIONS; CELL LINE; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; MASS SPECTROMETRY; PROTEOLYSIS; PROTEOME; PROTEOMICS;

DROSOPHILA MELANOGASTER;

EID: 84864090317     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2012.03.003     Document Type: Article

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