Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Käß, Friedrich ^a   Junne, Stefan ^b   Neubauer, Peter ^b   Wiechert, Wolfgang ^a   Oldiges, Marco ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Metabolic robustness; Oxygen supply limitation; Oxygen uptake redistribution; Scale down; STR PFR; Two compartment reactor

Indexed keywords

DISSOLVED OXYGEN; GLUCOSE; LACTIC ACID;

ACIDIFICATION; ARTICLE; CARBON UTILIZATION; CELL METABOLISM; CORYNEBACTERIUM GLUTAMICUM; FED BATCH REACTOR; NONHUMAN; OSCILLATION; OXYGEN CONCENTRATION; OXYGEN SUPPLY; PLUG FLOW REACTOR; STIRRED REACTOR; SYSTEMS BIOLOGY; TURNOVER TIME;

CORYNEBACTERIUM GLUTAMICUM;

BATCH CELL CULTURE TECHNIQUES; BIOMASS; BIOREACTORS; CARBON; CORYNEBACTERIUM GLUTAMICUM; GLUCOSE; OXYGEN;

EID: 84892396971     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-13-6     Document Type: Article

References (39)

1. Hermann T. Industrial production of amino acids by coryneform bacteria. J Biotechnol 2003, 104:155-172. 10.1016/S0168-1656(03)00149-4, 12948636.
2. van Ooyen J, Noack S, Bott M, Reth A, Eggeling L. Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnol Bioeng 2012, 109:2070-2081. 10.1002/bit.24486, 22392073.
3. Litsanov B, Kabus A, Brocker M, Bott M. Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum. Microb Biotechnol 2012, 5:116-128. 10.1111/j.1751-7915.2011.00310.x, 22018023.
4. Hasegawa S, Suda M, Uematsu K, Natsuma Y, Hiraga K, Jojima T, Inui M, Yukawa H. Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions. Appl Environ Microbiol 2013, 79:1250-1257. 10.1128/AEM.02806-12, 3568611, 23241971.
5. Bartek T, Blombach B, Zönnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M. Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum. Biotechnol Prog 2010, 26:361-371.
6. Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ. Corynebacterium glutamicum tailored for high-yield L-valine production. Appl Microbiol Biotechnol 2008, 79:471-479. 10.1007/s00253-008-1444-z, 18379776.
7. Niimi S, Suzuki N, Inui M, Yukawa H. Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. Appl Microbiol Biotechnol 2011, 90:1721-1729. 10.1007/s00253-011-3190-x, 21424269.
8. Jojima T, Fujii M, Mori E, Inui M, Yukawa H. Engineering of sugar metabolism of Corynebacterium glutamicum for production of amino acid L-alanine under oxygen deprivation. Appl Microbiol Biotechnol 2010, 87:159-165. 10.1007/s00253-010-2493-7, 20217078.
9. Okino S, Inui M, Yukawa H. Production of organic acids by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 2005, 68:475-480. 10.1007/s00253-005-1900-y, 15672268.
10. Wendisch VF, Bott M, Eikmanns BJ. Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids. Curr Opin Microbiol 2006, 9:268-274. 10.1016/j.mib.2006.03.001, 16617034.
11. Lara AR, Galindo E, Ramírez OT, Palomares LA. Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells. Mol Biotechnol 2006, 34:355-381. 10.1385/MB:34:3:355, 17284782.
12. Mayr B, Moser A, Nagy E, Horvat P. Scale-up on basis of structured mixing models: A new concept. Biotechnol Bioeng 1994, 43:195-206. 10.1002/bit.260430303, 18615651.
13. Tan R, Eberhard W, Büchs J. Measurement and characterization of mixing time in shake flasks. Chem Eng Sci 2011, 66:440-447.
14. Kawase Y, Moo-Young M. Mixing time in bioreactors. J Chem Technol Biotechnol 1989, 44:63-75.
15. Biopharmaceutical Production Technology 2012, Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, Subramanian G.
16. Neubauer P, Cruz N, Glauche F, Junne S, Knepper A, Raven M. Consistent development of bioprocesses from microliter cultures to the industrial scale. Eng Life Sci 2013, 13:224-238.
17. Neubauer P, Junne S. Scale-down simulators for metabolic analysis of large-scale bioprocesses. Curr Opin Biotechnol 2010, 21:114-121. 10.1016/j.copbio.2010.02.001, 20185293.
18. Takors R. Scale-up of microbial processes: impacts, tools and open questions. J Biotechnol 2012, 160:3-9. 10.1016/j.jbiotec.2011.12.010, 22206982.
19. Neubauer P, Ahman M, Törnkvist M, Larsson G, Enfors SO. Response of guanosine tetraphosphate to glucose fluctuations in fed-batch cultivations of Escherichia coli. J Biotechnol 1995, 43:195-204. 10.1016/0168-1656(95)00130-1, 8590645.
20. Lin HY, Neubauer P. Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli. J Biotechnol 2000, 79:27-37. 10.1016/S0168-1656(00)00217-0, 10817339.
21. Schilling, Pfefferle, Bachmann, Leuchtenberger, Deckwer. A special reactor design for investigations of mixing time effects in a scaled-down industrial L-lysine fed-batch fermentation process. Biotechnol Bioeng 1999, 64:599-606. 10.1002/(SICI)1097-0290(19990905)64:5<599:AID-BIT10>3.0.CO;2-C, 10404240.
22. Delvigne F, Destain J, Thonart P. A methodology for the design of scale-down bioreactors by the use of mixing and circulation stochastic models. Biochem Eng J 2006, 28:256-268.
23. Chamsartra S, Hewitt CJ, Nienow AW. The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor. Biotechnol Lett 2005, 27:693-700. 10.1007/s10529-005-4690-5, 16049736.
24. Bäumchen C, Knoll A, Husemann B, Seletzky J, Maier B, Dietrich C, Amoabediny G, Büchs J. Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate. J Biotechnol 2007, 128:868-874. 10.1016/j.jbiotec.2007.01.001, 17275119.
25. Junne S, Klingner A, Kabisch J, Schweder T, Neubauer P. A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivations. Biotechnol J 2011, 6:1009-1017. 10.1002/biot.201100293, 21751400.
26. Shinfuku Y, Sorpitiporn N, Sono M, Furusawa C, Hirasawa T, Shimizu H. Development and experimental verification of a genome-scale metabolic model for Corynebacterium glutamicum. Microb Cell Fact 2009, 8:43. 10.1186/1475-2859-8-43, 2728707, 19646286.
27. Paczia N, Nilgen A, Lehmann T, Gätgens J, Wiechert W, Noack S. Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms. Microb Cell Fact 2012, 11:122. 10.1186/1475-2859-11-122, 3526501, 22963408.
28. Enfors SO, Jahic M, Rozkov A, Xu B, Hecker M, Jürgen B, Krüger E, Schweder T, Hamer G, O'Beirne D, Noisommit-Rizzi N, Reuss M, Boone L, Hewitt C, McFarlane C, Nienow A, Kovacs T, Trägårdh C, Fuchs L, Revstedt J, Friberg PC, Hjertager B, Blomsten G, Skogman H, Hjort S, Hoeks F, Lin HY, Neubauer P, van der Lans R, Luyben K, et al. Physiological responses to mixing in large scale bioreactors. J Biotechnol 2001, 85:175-185. 10.1016/S0168-1656(00)00365-5, 11165362.
29. George S, Larsson G, Enfors S. A scale-down two-compartment reactor with controlled substrate oscillations: metabolic response of Saccharomyces cerevisiae. Bioprocess Biosyst Eng 1993, 9:249-257.
30. Inui M, Murakami S, Okino S, Kawaguchi H, Vertès AA, Yukawa H. Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions. J Mol Microbiol Biotechnol 2004, 7:182-196. 10.1159/000079827, 15383716.
31. Yamamoto S, Sakai M, Inui M, Yukawa H. Diversity of metabolic shift in response to oxygen deprivation in Corynebacterium glutamicum and its close relatives. Appl Microbiol Biotechnol 2011, 90:1051-1061. 10.1007/s00253-011-3144-3, 21327408.
32. Neubauer P, Häggström L, Enfors SO. Influence of substrate oscillations on acetate formation and growth yield in Escherichia coli glucose limited fed-batch cultivations. Biotechnol Bioeng 1995, 47:139-146. 10.1002/bit.260470204, 18623386.
33. Xu B, Jahic M, Blomsten G, Enfors SO. Glucose overflow metabolism and mixed-acid fermentation in aerobic large-scale fed-batch processes with Escherichia coli. Appl Microbiol Biotechnol 1999, 51:564-571. 10.1007/s002530051433, 10390814.
34. Stansen C, Uy D, Delaunay S, Eggeling L, Goergen J, Wendisch VF. Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Appl Environ Microbiol 2005, 71:5920-5928. 10.1128/AEM.71.10.5920-5928.2005, 1265975, 16204505.
35. Levenspiel O. Chemical reaction engineering 1972, New York: Wiley, 2.
36. Junne S, Nicolas Cruz-Bournazou M, Angersbach A, Götz P. Electrooptical monitoring of cell polarizability and cell size in aerobic Escherichia coli batch cultivations. J Ind Microbiol Biotechnol 2010, 37:935-942. 10.1007/s10295-010-0742-5, 20512396.
37. Tromans D. Modeling oxygen solubility in water and electrolyte solutions. Ind Eng Chem Res 2000, 39:805-812.
38. Narita E, Lawson F, Han K. Solubility of oxygen in aqueous electrolyte solutions. Hydrometallurgy 1983, 10:21-37.
39. Keilhauer C, Eggeling L, Sahm H. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J Bacteriol 1993, 175:5595-5603. 206616, 8366043.