Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

Genetics and Molecular Research

Volumn 13, Issue 1, 2014, Pages 205-219

  Rocejanasaroj, A ^a   Tencomnao, T ^a   Sangkitikomol, W ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

Antioxidant; CYP450; P glycoprotein; Thunbergia laurifolia

Indexed keywords

ANTIOXIDANT; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 2B6; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A4; CYTOCHROME P450 3A7; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LIVER X RECEPTOR ALPHA; LOW DENSITY LIPOPROTEIN RECEPTOR; MULTIDRUG RESISTANCE PROTEIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA1; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PLANT EXTRACT; THUNBERGIA LAURIFOLIA EXTRACT; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; BIOTRANSFORMATION; CELL STRAIN HEPG2; CELL SURVIVAL; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG CYTOTOXICITY; GENE EXPRESSION; HERB; HUMAN; HUMAN CELL; LIPID METABOLISM; OXIDATIVE STRESS; PLANT LEAF; THUNBERGIA LAURIFOLIA;

ACANTHACEAE; ANTIOXIDANTS; CELL SURVIVAL; CYTOCHROME P-450 ENZYME SYSTEM; DOSE-RESPONSE RELATIONSHIP, DRUG; HEP G2 CELLS; HUMANS; LIPID METABOLISM; P-GLYCOPROTEIN; PLANT EXTRACTS; PPAR GAMMA; RNA, MESSENGER;

EID: 84892384876     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2014.January.10.12     Document Type: Article

References (40)

1. Abass K, Turpeinen M and Pelkonen O (2009). An evaluation of the cytochrome P450 inhibition potential of selected pesticides in human hepatic microsomes. J. Environ. Sci. Health B 44: 553-563.
2. Bailey DG and Dresser GK (2004). Interactions between grapefruit juice and cardiovascular drugs. Am. J. Cardiovasc. Drugs 4: 281-297.
3. Butura A, Nilsson K, Morgan K, Morgan TR, et al. (2009). The impact of CYP2E1 on the development of alcoholic liver disease as studied in a transgenic mouse model. J. Hepatol. 50: 572-583.
4. Calkin AC and Tontonoz P (2010). Liver x receptor signaling pathways and atherosclerosis. Arterioscler. Thromb. Vasc. Biol. 30: 1513-1518.
5. Chen C, Yu R, Owuor ED and Kong AN (2000). Activation of antioxidant-response element (ARE), mitogen-activated protein kinases (MAPKs) and caspases by major green tea polyphenol components during cell survival and death. Arch. Pharm. Res. 23: 605-612.
6. Davalos A, Gomez-Cordoves C and Bartolome B (2004). Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay. J. Agric. Food Chem. 52: 48-54.
7. Everett L, Galli A and Crabb D (2000). The role of hepatic peroxisome proliferator-activated receptors (PPARs) in health and disease. Liver 20: 191-199.
8. Gasche Y, Daali Y, Fathi M, Chiappe A, et al. (2004). Codeine intoxication associated with ultrarapid CYP2D6 metabolism. N. Engl. J. Med. 351: 2827-2831.
9. Guengerich FP (2008). Cytochrome p450 and chemical toxicology. Chem. Res. Toxicol. 21: 70-83.
10. Hamidovic A, Hahn K and Kolesar J (2010). Clinical significance of ABCB1 genotyping in oncology. J. Oncol. Pharm. Pract. 16: 39-44.
11. Han FM, Yang QC, Du P and Wu WH (2009). The metabolism of strychnine in rat liver microsomes and metabolic interaction with glycyrrhetic acid in vitro. Asian J. Pharmacodyn. Pharmacokinet. 9: 301-312.
12. Huang D, Ou B and Prior RL (2005). The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53: 1841-1856.
13. Jaiboon V, Boonyanuphap J, Suwansri S, Ratanatraiwong P, et al. (2011). Alpha amylase inhibition and roasting time of local vegetables and herbs prepared for diabetes risk reducing chili paste. As. J. Food Ag.-Ind. 4: 103-113.
14. Jia JX and Wasan KM (2008). Effects of monoglycerides on rhodamine 123 accumulation, estradiol 17 beta-D-glucuronide bidirectional transport and MRP2 protein expression within Caco-2 cells. J. Pharm. Pharm. Sci. 11: 45-62.
15. Kaul D, Shukla AR, Sikand K and Dhawan V (2005). Effect of herbal polyphenols on atherogenic transcriptome. Mol. Cell Biochem. 278: 177-184.
16. Kim SN, Seo JY, Jung DW, Lee MY, et al. (2007). Induction of hepatic CYP2E1 by a subtoxic dose of acetaminophen in rats: increase in dichloromethane metabolism and carboxyhemoglobin elevation. Drug Metab. Dispos. 35: 1754-1758.
17. Kong AN, Yu R, Chen C, Mandlekar S, et al. (2000). Signal transduction events elicited by natural products: role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 23: 1-16.
18. Kumagai Y and Sumi D (2007). Arsenic: signal transduction, transcription factor, and biotransformation involved in cellular response and toxicity. Annu. Rev. Pharmacol. Toxicol. 47: 243-262.
19. Lander HM (1997). An essential role for free radicals and derived species in signal transduction. FASEB J. 11: 118-124.
20. Lin JH (2003). Drug-drug interaction mediated by inhibition and induction of P-glycoprotein. Adv. Drug Deliv. Rev. 55: 53-81.
21. Madabushi R, Frank B, Drewelow B, Derendorf H, et al. (2006). Hyperforin in St. John's wort drug interactions. Eur. J. Clin. Pharmacol. 62: 225-233.
22. Medina-Gomez G, Gray SL, Yetukuri L, Shimomura K, et al. (2007). PPAR gamma 2 prevents lipotoxicity by controlling adipose tissue expandability and peripheral lipid metabolism. PLoS Genet. 3: e64.
23. Oonsivilai R, Cheng C, Bomser J, Ferruzzi MG, et al. (2007). Phytochemical profiling and phase II enzyme-inducing properties of Thunbergia laurifolia Lindl. (RC) extracts. J. Ethnopharmacol. 114: 300-306.
24. Powell EE and Kroon PA (1994). Low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression in human mononuclear leukocytes is regulated coordinately and parallels gene expression in human liver. J. Clin. Invest. 93: 2168-2174.
25. Pramyothin P, Chirdchupunsare H, Rungsipipat A and Chaichantipyuth C (2005). Hepatoprotective activity of Thunbergia laurifolia Linn extract in rats treated with ethanol: in vitro and in vivo studies. J. Ethnopharmacol. 102: 408-411.
26. Sangkitikomol W, Tencomnao T and Rocejanasaroj A (2010). Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells. Genet. Mol. Res. 9: 2086-2095.
27. Sen S, Chakraborty R, Sridhar C and Reddy YSR (2010). Free radicals, antioxidants, diseases and phytomedicines: Current status and future prospect. IJPSR 3: 91-100.
28. Shimada T, Kojima K, Yoshiura K, Hiraishi H, et al. (2002). Characteristics of the peroxisome proliferator activated receptor gamma (PPARgamma) ligand induced apoptosis in colon cancer cells. Gut 50: 658-664.
29. Singleton VL, Orthofer R and Lamuela-Raventos RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol. 299: 152-178.
30. Takahashi S, Takahashi T, Mizobuchi S, Matsumi M, et al. (2002). Increased cytotoxicity of carbon tetrachloride in a human hepatoma cell line overexpressing cytochrome P450 2E1. J. Int. Med. Res. 30: 400-405.
31. Tejasen P and Thongthapp C (1980). The study of the insecticide antitoxicity of Thunbergia laurifolia Linn. Chiang Mai Med. Bull. 19: 105-114.
32. Twentyman PR and Luscombe M (1987). A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br. J. Cancer 56: 279-285.
33. Wang H and Joseph JA (1999). Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic. Biol. Med. 27: 612-616.
34. Watanabe K, Yamaori S, Funahashi T, Kimura T, et al. (2007). Cytochrome P450 enzymes involved in the metabolism of tetrahydrocannabinols and cannabinol by human hepatic microsomes. Life Sci. 80: 1415-1419.
35. Wenk M, Todesco L and Krahenbuhl S (2004). Effect of St John's wort on the activities of CYP1A2, CYP3A4, CYP2D6, N-acetyltransferase 2, and xanthine oxidase in healthy males and females. Br. J. Clin. Pharmacol. 57: 495-499.
36. Williams RJ, Spencer JP and Rice-Evans C (2004). Flavonoids: antioxidants or signalling molecules? Free Radic. Biol. Med. 36: 838-849.
37. Willson TM, Lambert MH and Kliewer SA (2001). Peroxisome proliferator-activated receptor gamma and metabolic disease. Annu. Rev. Biochem. 70: 341-367.
38. Wonkchalee O, Boonmars T, Aromdee C, Laummaunwai P, et al. (2012). Anti-inflammatory, antioxidant and hepatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis. Parasitol. Res. 111: 353-359.
39. Woodcroft KJ, Hafner MS and Novak RF (2002). Insulin signaling in the transcriptional and posttranscriptional regulation of CYP2E1 expression. Hepatology 35: 263-273.
40. Zhang SZ, Lipsky MM, Trump BF and Hsu IC (1990). Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell Biol. Toxicol. 6: 219-234.