Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system

Applied Microbiology and Biotechnology

Volumn 98, Issue 1, 2014, Pages 313-323

  Cui, Gu Zhen ^a   Zhang, Jie ^a,b   Hong, Wei ^a,b   Xu, Chenggang ^a   Feng, Yingang ^a   Cui, Qiu ^a   Liu, Ya Jun ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Clostridium cellulolyticum; ClosTron; Plasmid curing; PyrF; Targetron

Indexed keywords

ANTIBIOTICS; BIOFUELS; CURING; DNA; EFFICIENCY; GENES; GENETIC ENGINEERING; METABOLIC ENGINEERING;

CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM THERMOCELLUM; CLOSTRON; ERYTHROMYCIN RESISTANCE; EXPRESSION CASSETTE; HOMOLOGOUS RECOMBINATION; PYRF; TARGETRON;

CLOSTRIDIUM;

BIOFUEL; CIPC PROTEIN; ERYTHROMYCIN; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; BACTERIUM; BIOFUEL; BIOTECHNOLOGY; GENE EXPRESSION; GENETIC ENGINEERING; INDUSTRIAL PRODUCTION; METABOLISM; PLASMID; TRANSFORMATION;

ARTICLE; BACTERIAL GROWTH; CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM PASTEURIANUM; GENE DISRUPTION; GENE TARGETING; GENETIC ENGINEERING; GENETIC MANIPULATION; HOMOLOGOUS RECOMBINATION; NONHUMAN; PLASMID; SCREENING;

CLOSTRIDIUM; CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM THERMOCELLUM;

CLOSTRIDIUM CELLULOLYTICUM; GENE KNOCKOUT TECHNIQUES; OROTIC ACID; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; PLASMIDS; SELECTION, GENETIC;

EID: 84891883165     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5330-y     Document Type: Article

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