Development of pyrF-based gene knockout systems for genome-wide manipulation of the archaea Haloferax mediterranei and Haloarcula hispanica

Journal of Genetics and Genomics

Volumn 38, Issue 6, 2011, Pages 261-269

  Liu, Hailong ^a   Han, Jing ^a   Liu, Xiaoqing ^a   Zhou, Jian ^a   Xiang, Hua ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Haloarchaea; Knockout system; Pop in pop out method; PyrF

Indexed keywords

FLUOROOROTIC ACID; PHYTOENE SYNTHASE; PLASMID VECTOR;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA FLANKING REGION; GENE DELETION; GENE INACTIVATION; GENETIC ANALYSIS; GENETIC MANIPULATION; GENETIC SELECTION; HALOARCULA HISPANICA; HALOFERAX MEDITERRANEI; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PYRF GENE; SOUTHERN BLOTTING; SUICIDE GENE;

ALKYL AND ARYL TRANSFERASES; AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; CULTURE MEDIA; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; HALOARCULA; HALOFERAX MEDITERRANEI; MOLECULAR SEQUENCE DATA; OROTIC ACID; PLASMIDS; RECOMBINATION, GENETIC; SEQUENCE DELETION; URACIL;

HALOARCULA HISPANICA; HALOBACTERIA; HALOFERAX MEDITERRANEI;

EID: 79959374305     PISSN: 16738527     EISSN: 18735533     Source Type: Journal    
DOI: 10.1016/j.jgg.2011.05.003     Document Type: Article

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