Inhibition of DNA-dependent protein kinase reduced palmitate and oleate-induced lipid accumulation in HepG2 cells

European Journal of Nutrition

Volumn 52, Issue 6, 2013, Pages 1621-1630

  Kwan, HiuYee ^a   Fong, Wang Fun ^a   Yang, Zhijun ^a   Yu, Zhi Ling ^a   Hsiao, Wen Luan Wendy ^a  

^a HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

Author keywords

DNA PK; Hepatocellular steatosis; Lipid; Oleate; Palmitate; PP2A

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; DNA DEPENDENT PROTEIN KINASE; FATTY ACID SYNTHASE; LIPID; MESSENGER RNA; OKADAIC ACID; OLEIC ACID; PALMITIC ACID; PHOSPHOPROTEIN PHOSPHATASE 2A; SMALL INTERFERING RNA; STEROL REGULATORY ELEMENT BINDING PROTEIN 1;

APOPTOSIS; ARTICLE; BIOACCUMULATION; CELL STRAIN HEPG2; CELL VIABILITY; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME ACTIVITY; ENZYME INHIBITION; FATTY LIVER; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE SILENCING; LIPID STORAGE; LIVER CELL; LUCIFERASE ASSAY; MTT ASSAY; PROMOTER REGION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; STAINING; UPREGULATION; WESTERN BLOTTING;

APOPTOSIS; CELL SURVIVAL; DNA FRAGMENTATION; DNA-ACTIVATED PROTEIN KINASE; FATTY ACID SYNTHASES; HEP G2 CELLS; HEPATOCYTES; HUMANS; LIPID METABOLISM; OLEIC ACID; PALMITIC ACID; PHOSPHORYLATION; PROMOTER REGIONS, GENETIC; PROTEIN PHOSPHATASE 2; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; STEROL REGULATORY ELEMENT BINDING PROTEIN 1; UP-REGULATION;

EID: 84891830448     PISSN: 14366207     EISSN: 14366215     Source Type: Journal    
DOI: 10.1007/s00394-012-0467-5     Document Type: Article

References (35)

1. Brunt EM (2004) Nonalcoholic steatohepatitis. Semin Liver Dis 24:3-20
2. Cohen JC, Horton JD, Hobbs HH (2011) Human fatty liver disease: old questions and new insights. Science 32:1519-1523
3. Starley BQ, Calcagno CJ, Harrison SA (2010) Nonalcoholic fatty liver disease and hepatocellular carcinoma: a weighty connection. Hepatology 51:1820-1832
4. Ahmed MH, Byrne CD (2009) Current treatment of non-alcoholic fatty liver disease. Diabetes Obes Metab 11:188-195
5. Gentile CL, Pahliassotti MJ (2008) The role of fatty acids in the development and progression of nonalcoholic fatty liver disease. J Nutr Biochem 19:567-576
6. Abraham RT (2004) PI 3-kinase related kinases: big players in stress-induced signaling pathways. DNA Repair 3:883-887
7. Jin S, Kharbanda S, Mayer B, Kufe D, Weaver DT (1997) Binding of Ku and c-Abl at the kinase homology region of DNA-PK catalytic subunit. J Biol Chem 272:24763-24766
8. Chan DW, Lees-Miller SP (1996) The DNA-PK is inactivated by autophosphorylation of the catalytic subunit. J Biol Chem 271:8936-8941
9. Wechsler T, Chen BP, Harper R, Morotomi-Yano K, Huang BC, Meek K, Cleaver JE, Chen DJ, Wabl M (2004) DNA-PKcs function regulated specifically by protein phosphatase 5. PNAS 101:1247-1252
10. Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS (2009) A role of DNA-PK for the metabolic gene regulation in response to insulin. Cell 136:1056-1072
11. Wu Y, Song P, Xu J, Zhang M, Zou MH (2007) Activation of protein phosphatase 2A by palmitate inhibits AMP-activated protein kinase. J Biol Chem 282:9777-9788
12. Douglas P, Moorhead GB, Ye R, Lees-Miller SP (2001) Protein phosphatases regulate DNA-PK activity. J Biol Chem 276:18992-18998
13. Douglas P, Zhong J, Ye R, Moorhead GB, Xu X, Lees-Miller SP (2010) Protein phosphatase 6 interacts with the DNA-dependent protein kinas catalytic subunit and dephosphorylates gamma-H2AX. Mol Cell Biol 30:1368-1381
14. Wang Q, Gao F, Wang T, Flagg T, Deng X (2009) A nonhomologous end-joining pathway is required for protein phosphatase 2A promotion of DNA double-strand break repair. Neoplasia 11:1012-1021
15. Wang H, Chan PK, Pan SY, Kwon KH, Ye Y, Chu JH, Fong WF, Tsui WM, Yu ZL (2010) ERp57 is up-regulated in free fatty acids-induced steatotic L-02 cells and human nonalcoholic fatty livers. J Cell Biochem 110:1447-1456
16. Chu JH, Wang H, Ye Y, Chan PK, Pan SY, Fong WF, Yu ZL (2011) Inhibitory effect of schisandrin B on free fatty acid-induced steatosis in L-02 cells. World J Gastroenterol 17:2379-2388
17. Li YW, Zhu GY, Shen XL, Chu HJ, Yu ZL, Fong WF (2011) Furanodienone inhibits cell proliferation and survival by suppressing ERα signaling in human breast cancer MCF-7 cells. J Cell Biochem 112:217-224
18. Jackel-Cram C, Babiuk LA, Liu Q (2007) Up-regulation of fatty acid synthase promoter by hepatitis C virus core protein: genotype-3a core has a stronger effect than genotype-1b core. J Hepatol 46:999-1008
19. Srivastava S, Chan C (2007) Hydrogen peroxide and hydroxyl radicals mediate palmitate-induced cytotoxicity to hepatoma cells: relation to mitochondrial permeability transition. Free Radic Res 41:38-49
20. Oosterveer MH, van Dijk TH, Tietge UJ, Boer T, Havinga R, Stellaard F, Groen AK, Kuipes F, Reijngoud DJ (2009) High fat feeding induces hepatic fatty acid elongation in mice. PLoS One 4:e6066
21. Donnelly KL, Smith Ci, Schwarzenberg SJ, Jessurun J, Boldt MD, Parks EJ (2005) Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty acid liver disease. J Clin Invest 115:1343-1351
22. Sanyal AJ (2005) Mechanisms of disease pathogenesis of nonalcoholic fatty liver disease. Nat Clin Pract Gastroenterol Hepatol 2:46-53
23. Nakamuta M, Kohjima M, Morizono S, Kotoh K, Yoshimoto Y, Miyagi I, Enjoji M (2005) Evaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease. Int J Mol Med 16:631
24. Willmore E, de Caux S, Sunter NJ, Tilby MJ, Jackson GH, Austin CA, Durkacz BW (2004) A novel DNA-PK inhibitor, NU7026, potentiates the cytotoxicity of topoisomerase II poisons used in the treatment of leukemia. Blood 103:4659-4665
25. Kanungo J (2010) Exogenously expressed human Ku70 stabilizes Ku80 in Xenopus oocytes and induces heterologous DNA-PK catalytic activity. Mol Cell Biochem 338:291
26. Marra F, Gastaldelli A, Svegliati Baroni G, Tell G, Triibelli C (2008) Molecular basis and mechanisms of progression of nonalcoholic steatohepatitis. Trends Mol Med 14:72-81
27. Galbo T, Olsen GS, Quistorff B, Nishimura E (2011) Free fatty acid-induced PP2A hyperactivity selectively impairs hepatic insulin action on glucose metabolism. PLoS One 6:e27424
28. Cazzolli R, Carpenter L, Biden TJ, Schmitz-Peiffer C (2011) A role for protein phosphatase 2A-like activity, but not atypical protein kinase Czeta, in the inhibition of protein kinase B/Akt and glycogen synthesis by palmitate. Diabetes 50:2210-2218
29. Miglietta A, Bozzo F, Gabriel L, Bocca C, Canuto RA (2006) Extracellular signal-regulated kinase 1/2 and protein phosphatase 2A are involved in the antiproliferative activity of conjugated linoleic acid in MCF-7 cells. Br J Nutr 96:22-27
30. Shimizu S, Ugi S, Maegawa H, Eqawa K, Nishio Y, Yoshizaki T, Shi K, Nagai Y, Morino K, Nemoto K, Nakamura T, Bryer-Ash M, Kashiwagi A (2003) Protein-tyrosine phosphatase 1B as new activator for hepatic lipogenesis via sterol regulatory element-binding protein-1 gene expression. J Biol Chem 278:43095-43101
31. Mueller AS, Klomann SD, Wolf NM, Schneider S, Schmidt R, Spielmann J, Stangl G, Eder K, Pallauf J (2008) Redox regulation of protein tyrosine phosphatase 1B by manipulation of dietary selenium affects the lipid concentration in rat liver. J Nutr 138:2328-2336
32. Shi K, Ugi S, Shimizu S, Sekine O, Ikede K, Eqawa K, Yoshizaki Y, Nagai Y, Nishio Y, Takada T, Torii R, Kimura H, Kashiwagi A, Maegawa H (2007) Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression. Biochem Biophys Res Commun 363:626-632
33. Sampath H, Miyazaki M, Dobrzyn A, Ntambi JM (2007) Stearoyl-CoA desaturase-1 mediates the pro-lipogenic effects of dietary saturated fat. J Biol Chem 282:2483-2493
34. Biddinger SB, Almind K, Miyazaki M, Kokkotou E, Ntambi JM, Kahn CR (2005) Effects of diet and genetic background on sterol regulatory element-binding protein-1c, stearoyl-CoA desaturase 1, and the development of the metabolic syndrome. Diabetes 54:1314-1323
35. Lin J, Yang R, Tarr PT, Wu PH, Handschin C, Li S, Yang W, Pei L, Uldry M, Tontonoz P, Newgard CB, Spiegelman BM (2005) Hyperlipidemic effects of dietary saturated fats mediated through PGC-1beta coactivation of SREBP. Cell 120:261-273