An engineered Fc variant of an IgG eliminates all immune effector functions via structural perturbations

Methods

Volumn 65, Issue 1, 2014, Pages 114-126

  Vafa, Omid ^a   Gilliland, Gary L ^a   Brezski, Randall J ^a   Strake, Brandy ^a   Wilkinson, Teresa ^a   Lacy, Eilyn R ^a   Scallon, Bernard ^a   Teplyakov, Alexey ^a   Malia, Thomas J ^a   Strohl, William R ^a  

^a JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

Author keywords

Apoptosis; Complement; Cytokine release; Effector function; Fc receptors; IgG; Immunoglobulin Fc; Immunostimulatory; Isotype; X ray structure

Indexed keywords

CD20 ANTIBODY; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G2 DELTA; IMMUNOGLOBULIN G4; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTIBODY AFFINITY; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ARTICLE; CELL STRAIN HEK293; CHO CELL; COMPLEMENT ACTIVATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOTOXICITY; DENSITY GRADIENT; DRUG HALF LIFE; IMMUNE RESPONSE; IN VITRO STUDY; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PHAGOCYTOSIS; PLASMA HALF LIFE; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN SECRETION; PROTEIN STRUCTURE;

2,2′:6′,2″-TERPYRIDINE-6,6″-DICARBOXYLIC ACID; ADCC; ADCP; ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY; ANTIBODY-DEPENDENT CELLULAR PHAGOCYTOSIS; APOPTOSIS; BOVINE SERUM ALBUMIN; BSA; CDC; COMPLEMENT; COMPLEMENT DEPENDENT CYTOTOXICITY; CYTOKINE RELEASE; D-PBS; DULBECCO'S PHOSPHATE-BUFFERED SALINE; ECD; EFFECTOR FUNCTION; EXTRACELLULAR DOMAIN; FACS; FBS; FC GAMMA RECEPTOR; FC RECEPTORS; FC-DEPENDENT INDUCTION OF CYTOKINE RELEASE; FCΓR; FETAL BOVINE SERUM; FIC; FLUORESCENCE-ACTIVATED CELL SORTER; GM-CSF; GRANULOCYTE/MACROPHAGE COLONY STIMULATING FACTOR; IGG; IMMUNOGLOBULIN FC; IMMUNOGLOBULIN G; IMMUNOSTIMULATORY; ISOTYPE; KEYHOLE LIMPET HEMOCYANIN; KLH; MAB; MAC; MEMBRANE ATTACK COMPLEX; MESO SCALE DISCOVERY; MONOCLONAL ANTIBODY; MSD; PBMCS; PBS; PERIPHERAL BLOOD MONONUCLEAR CELLS; PHARMACOKINETICS; PHOSPHATE BUFFERED SALINE; PK; SPR; SURFACE PLASMON RESONANCE; TDA; X-RAY STRUCTURE;

AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY-DEPENDENT CELL CYTOTOXICITY; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYTOKINES; HALF-LIFE; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; IMMUNOLOGIC FACTORS; MACACA FASCICULARIS; MALE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; RECEPTOR, ERBB-2; RECEPTORS, IGG;

EID: 84891631658     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2013.06.035     Document Type: Article

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