Progress in plant protoplast research

Planta

Volumn 238, Issue 6, 2013, Pages 991-1003

  Eeckhaut, Tom ^a   Lakshmanan, Prabhu Shankar ^a,b   Deryckere, Dieter ^a   Van Bockstaele, Erik ^a,b   Van Huylenbroeck, Johan ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Genome fragmentation; Hybrid screening; Interspecific hybridization; Protoplast regeneration; Somatic fusion

Indexed keywords

PROTEOME; TRANSCRIPTOME;

CROP; CYTOLOGY; GENETICS; HYBRIDIZATION; PHYSIOLOGY; PLANT; PLANT BREEDING; PLANT CELL; PLANT GENOME; PROTOPLAST; REGENERATION;

CROPS, AGRICULTURAL; GENOME, PLANT; HYBRIDIZATION, GENETIC; PLANT BREEDING; PLANT CELLS; PLANTS; PROTEOME; PROTOPLASTS; REGENERATION; TRANSCRIPTOME;

EID: 84891162170     PISSN: 00320935     EISSN: 14322048     Source Type: Journal    
DOI: 10.1007/s00425-013-1936-7     Document Type: Review

References (146)

1. Abas Y, Touil N, Kirsch-Volders M, Angenon G, Jacobs M, Famelaer I (2007) Evaluation of UV damage at DNA level in Nicotiana plumbaginifolia protoplasts using single cell gel electrophoresis. Plant Cell Tiss Org 91: 145-154.
2. Azad M, Yokota S, Ishiguri F, Yoshizawa N (2006) Plant regeneration from mesophyll protoplasts of a medicinal plant, Phellodendron amurense Rupr. In Vitro Cell Dev Plant 42: 502-507.
3. Bajaj Y (1989) Plant protoplasts and genetic engineering. Springer, Heidelberg.
4. Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R (2007) Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tiss Org 91: 179-189.
5. Borgato L, Conicella C, Pisani F, Furini A (2007) Production and characterization of arboreous and fertile Solanum melongena plus Solanum marginatum somatic hybrid plants. Planta 226: 961-969.
6. Briere C, Barthou H, Jauneau A, Alibert G, Petitprez M (2004) The actin cytoskeleton is involved in signalling protoplast embryogenesis induced by agarose embedding. Physiol Plantarum 122: 115-122.
7. Cai X, Liu X, Guo W (2006) GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage. Plant Cell Tiss Org 87: 245-253.
8. Cassells A, Curry R (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org 64: 145-157.
9. Castelblanque L, Garcia-Sogo B, Pineda B, Moreno V (2010) Efficient plant regeneration from protoplasts of Kalanchoe blossfeldiana via organogenesis. Plant Cell Tiss Org 100: 107-112.
10. Chabane D, Assani A, Bouguedoura N, Haicour R, Ducreux G (2007) Induction of callus formation from difficile date palm protoplasts by means of nurse culture. C R Biol 330: 392-401.
11. Chen L, Zhang M, Xiao Q, Wu J, Hirata Y (2004) Plant regeneration from hypocotyl protoplasts of red cabbage (Brassica oleracea) by using nurse cultures. Plant Cell Tiss Org 77: 133-138.
12. Cheng Y, Guo W, Deng X (2003) Molecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal Valencia orange (Citrus sinensis) + Meiwa kumquat (Fortunella crassifolia) intergeneric somatic hybrids. Plant Cell Rep 21: 445-451.
13. Cheng Y, De Carmen V, Meng H, Guo W, Tao N, Deng X (2005) A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. Tree Physiol 25: 661-672.
14. Chevre A, Eber F, Margale E, Kerlan M, Primard C, Vedel F, Delseny M, Pelletier G (1994) Comparison of somatic and sexual Brassica napus-Sinapis alba hybrids and their progeny by cytogenetic studies and molecular characterization. Genome 37: 367-374.
15. Conde P, Santos C (2006) An efficient protocol for Ulmus minor Mill. Protoplast isolation and culture in agarose droplets. Plant Cell Tiss Org 86: 359-366.
16. Cui H, Yu Z, Deng J, Gao X, Sun Y, Xia G (2009) Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta 229: 323-330.
17. Dai X, Xiao W, Huang X, Zhao J, Chen Y, Huang X (2010) Plant regeneration from embryogenic cell suspensions and protoplasts of dessert banana cv. 'Da Jiao' (Musa paradisiacal ABB Linn.) via somatic embryogenesis. In Vitro Cell Dev Plant 46: 403-410.
18. Dambier D, Benyahia H, Pensabene-Bellavia G, Kacar Y, Froelicher Y, Belfalah Z, Lhou B, Handaji N, Printz B, Morillon R, Yesiloglu T, Navarro L, Ollitrault P (2011) Somatic hybridization for Citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean Citrus industry. Plant Cell Rep 30: 883-900.
19. Davey M, Anthony P, Power J, Lowe K (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv 23: 131-171.
20. Davey M, Anthony P, Patel D, Power J (2010) Plant Protoplasts: Isolation, Culture and Plant Regeneration. In: Davey M, Anthony P (eds) Plant cell culture: essential methods. John Wiley & Sons, New York, pp 153-173.
21. De Jong H (2003) Visualising DNA domains and sequences by microscopy: a fifty-year history of molecular cytogenetics. Genome 46: 943-946.
22. De Jong F, Mathesius U, Imin N, Rolfe B (2007) A proteome study of the proliferation of cultured Medicago truncatula protoplasts. Proteomics 7: 722-736.
23. Deryckere D, Eeckhaut T, van Huylenbroeck J, van Bockstaele E (2012) Low melting point agarose beads as a standard method for plantlet regeneration from protoplasts within the Cichorium genus. Plant Cell Rep 31: 2261-2269.
24. Deryckere D, De Keyser E, Eeckhaut T, van Huylenbroeck J, van Bockstaele E (2013) High-resolution melting analysis as a rapid and highly sensitive method for Cichorium plasmotype characterization. Plant Mol Biol Rep 31: 731-740.
25. Duquenne B, Eeckhaut T, Werbrouck S, van Huylenbroeck J (2007) Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium. Plant Cell Tiss Org 91: 165-173.
26. Eeckhaut T, van Huylenbroeck J (2011) Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts. Acta Physiol Plant 33: 1547-1551.
27. Escalante A, Imanishi S, Hossain M, Ohmido N, Fukui K (1998) RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides. Theor Appl Genet 96: 719-726.
28. Famelaer I, Verhoeven H, Dijkhuis P, Ramulu K (2007) A study of the process of synchronisation and micronucleation in Beta vulgaris and the monitoring of an isolation procedure for micro-nuclei and micro-protoplasts by confocal laser scanning microscopy and flow cytometry. Plant Cell Tiss Org Cult 90: 169-179.
29. Feng Y, Takahashi H, Akagi H, Mori K (2006) New interspecific rice genome constructions obtained by symmetric cell fusion. Plant Breed 125: 403-404.
30. Fitter J, Thomas M, Niu C, Rose R (2005) Investigation of Nicotiana tabacum + N. suaveolens cybrids with carpelloid stamens. J Plant Physiol 162: 225-235.
31. Fiuk A, Rybczynski J (2007) The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle). Plant Cell Tiss Org 91: 263-271.
32. Fu C, Chen C, Guo W, Deng X (2004) GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata. Plant Cell Rep 23: 391-396.
33. Gancle A, Grimplet J, Sauvage F, Ollitrault P, Brillouet J (2006) Predominant expression of diploid mandarin leaf proteome in two citrus mandarin-derived somatic allotetraploid hybrids. J Agr Food Chem 54: 6212-6218.
34. Grosser J, Gmitter F (2005) Applications of somatic hybridization and cybridization in crop improvement, with Citrus as a model. In Vitro Cell Dev Plant 41: 220-225.
35. Grosser J, Calovic M, Louzada E (2010) Protoplast fusion technology-somatic hybridization and cybridization. In: Davey M, Anthony P (eds) Plant cell culture: essential methods. John Wiley & Sons, New York, pp 175-198.
36. Grzebelus E, Szklarczyk M, Baranski R (2012a) An improved protocol for plant regeneration from leaf and hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Org 109: 101-109.
37. Grzebelus E, Szklarczyk M, Gren J, Sniegowska K, Jopek M, Kacinska I, Mrozek K (2012b) Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures. Plant Growth Regul 67: 93-100.
38. Guo W, Grosser J (2005) Somatic hylbrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Sci 168: 1541-1545.
39. Guo W, Prasad D, Cheng Y, Serrano P, Deng X, Grosser J (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Rep 22: 752-758.
40. Guo J, Liu Q, Zhai H, Wang Y (2006) Regeneration of plants from Ipomoea cairica L. protoplasts and production of somatic hybrids between I. cairica L. and sweetpotato, I. batatas (L.) Lam. Plant Cell Tiss Org 87: 321-327.
41. Guo W, Wu R, Cheng Y, Deng X (2007a) Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Breed 126: 72-76.
42. Guo Y, Bai J, Zhang Z (2007b) Plant regeneration from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc.). Plant Cell Tiss Org 89: 151-157.
43. Guo W, Wu R, Cheng Y, Deng X (2008) Regeneration and molecular characterisation of two interspecific somatic hybrids of Citrus for potential rootstock improvement. J Hortic Sci Biotech 83: 407-410.
44. Guo X, Xie C, Cai X, Song B, He L, Liu J (2010) Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Rep 29: 1277-1285.
45. Hall N (2007) Advanced sequencing technologies and their wider impact in microbiology. J Exp Biol 210: 1518-1525.
46. Hall R, Krens F, Rouwendal G (1992) DNA radiation-damage and asymmetric somatic hybridization-is UV a potential substitute or supplement to ionizing-radiation in fusion experiments. Physiol Plant 85: 319-324.
47. He G, Zhang J, Li K, Xiong Z, Chen M, Chang J, Wang Y, Yang G, Barnabas B (2006) An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.). Plant Cell Tiss Org 86: 15-25.
48. Holme I, Torp A, Hansen L, Andersen S (2004) Quantitative trait loci affecting plant regeneration from protoplasts of Brassica oleracea. Theor Appl Genet 108: 1513-1520.
49. Inoue K, Kato T, Kunitake H, Yabuya T (2004) Efficient production of polyploid plants via protoplast culture of Iris fulva. Cytologia 69: 327-333.
50. Iovene M, Savarese S, Cardi T, Frusciante L, Scotti N, Simon P, Carputo D (2007) Nuclear and cytoplasmic genome composition of Solanum bulbocastanum + S. tuberosum somatic hybrids. Genome 50: 443-450.
51. Iovene M, Aversano R, Savarese S, Caruso I, Di Matteo A, Cardi T, Frusciante L, Carputo D (2012) Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding. Biol Plant 56: 1-8.
52. Ishii S (1987) Generation of active oxygen species during enzymic isolation of protoplasts from oat leaves. In Vitro Cell Dev Plant 23: 653-658.
53. Jiang J, Zhao X, Tian W, Li T, Wang Y (2009) Intertribal somatic hybrids between Brassica napus and Camelina sativa with high linolenic acid content. Plant Cell Tiss Org 99: 91-95.
54. Jiang L, Cai Y, Xia G, Xiang F (2012) Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization. Protoplasma 249: 737-745.
55. Johnson A, Veilleux R (2001) Somatic hybridization and application in plant breeding. In: Janick J (ed) Plant Breeding Rev 20. John Wiley & Sons, New York, pp 167-225.
56. Johnston S, Den Nijs A, Peloquin S, Hannemann R (1980) The significance of genetic balance to endosperm development in interspecific crosses. Theor Appl Genet 57: 5-9.
57. Kakoniova D, Hlinkova E, Liskova D, Kollarova K (2010) Oligosaccharides induce changes in protein patterns of regenerating spruce protoplasts. Cent Eur J Biol 5: 353-363.
58. Kanwar K, Bhardwaj A, Deepika R (2009) Efficient regeneration of plantlets from callus and mesophyll derived protoplasts of Robinia pseudoacacia L. Plant Cell Tiss Org 96: 95-103.
59. Kielkowska A, Adamus A (2012) An alginate-layer technique for culture of Brassica oleracea L. protoplasts. In Vitro Cell Dev Plant 48: 265-273.
60. Kim J, Bergervoet J, Raemakers C, Jacobsen E, Visser R (2005) Isolation of protoplasts, and culture and regeneration into plants in Alstroemeria. In Vitro Cell Dev Plant 41: 505-510.
61. Klocke E, Weinzierl C, Abel S (2012) Protoplast fusion for the generation of unique Pelargonium plants. Acta Hort 953: 119-127.
62. Ko J, Ju J, Lee S, Cha H (2006) Tobacco protoplast culture in a polydimethylsiloxane-based microfluidic channel. Protoplasma 227: 237-240.
63. Komai F, Morohashi H, Horita M (2006) Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum Thunb. In Vitro Cell Dev Plant 42: 252-255.
64. Kwon H, Yokoyama R, Nishitani K (2005) A proteomic approach to apoplastic proteins involved in cell wall regeneration in protoplasts of Arabidopsis suspension-cultured cells. Plant Cell Physiol 46: 843-857.
65. Lakshmanan P, Eeckhaut T, van Huylenbroeck J, van Bockstaele E (2013) Micronucleation by mitosis inhibitors in developing microspores of Spathiphyllum wallisii Regel. Plant Cell Rep 32: 369-377.
66. Li C, Xia G, Xiang F, Zhou C, Cheng A (2004) Regeneration of asymmetric somatic hybrid plants from the fusion of two types of wheat with Russian wildrye. Plant Cell Rep 23: 461-467.
67. Lian Y, Lin G, Zhao X, Lim H (2011) Production and genetic characterization of somatic hybrids between leaf mustard (Brassica juncea) and broccoli (Brassica oleracea). In Vitro Cell Dev Plant 47: 289-296.
68. Lian Y, Zhao X, Lin G, Lim H (2012) Protoplast isolation and culture for somatic hybridisation of rapid cycling Brassica rapa with 'Anand' CMS and Brassica juncea. Plant Cell Tiss Org 109: 565-572.
69. Liu C, Li S, Wang M, Xia G (2012) A transcriptomic analysis reveals the nature of salinity tolerance of a wheat introgression line. Plant Mol Biol 78: 159-169.
70. Meyer L, Serek M, Winkelmann T (2009) Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa. Plant Cell Tiss Org 99: 27-34.
71. Moricová P, Ondrěj V, Navrátilová B, Luhová L (2013) Changes of DNA methylation and hydroxymethylation in plant protoplast cultures. Acta Biochim Pol 60: 33-36.
72. Motte H, Galuszka P, Spíchal L, Tarkowski P, Plíhal O, Šmehilová M, Jaworek P, Vereecke D, Werbrouck S, Geelen D (2013) Phenyl-adenine, identified in a light-dependent short hypocotyls4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of cytokinin oxidase/dehydrogenase activity. Plant Physiol 163: 1229-1241.
73. Niedz R (2006) Regeneration of somatic embryos from sweet orange (C. sinensis) protoplasts using semi-permeable membranes. Plant Cell Tiss Org 84: 353-357.
74. Olivares-Fuster O, Duran-Vila N, Navarro L (2005) Electrochemical protoplast fusion in Citrus. Plant Cell Rep 24: 112-119.
75. Ondrěj V, Navrátilová B, Lebeda A (2009) The heterochromatin as a marker for protoplast differentiation of Cucumis sativus. Plant Cell Tiss Org 96: 229-234.
76. Ondrěj V, Navrátilová B, Protivánková I, Piterková J, Sedlárová M, Luhová L, Lebeda A (2010) Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress. J Exp Biol 61: 2395-2401.
77. Ovcharenko O, Momot V, Cherep N, Sheludko Y, Komarnitsky I, Rudas V, Kuchuk N (2011) Transfer of transformed Lesquerella fendleri (Gray) Wats. chloroplasts into Orychophragmus violaceus (L.) OE Schulz by protoplast fusion. Plant Cell Tiss Org 105: 21-27.
78. Pan Z, Liu C, Zobayed S, Saxena P (2004) Plant Regeneration from mesophyll protoplasts of Echinacea Purpurea. Plant Cell Tiss Org 77: 251-255.
79. Pan Z, Liu C, Murch S, Saxena P (2005) Optimized chemodiversity in protoplast-derived lines of St. John's wort (Hypericum perforatum L.). In Vitro Cell Dev Plant 41: 226-231.
80. Papadakis A, Roubelakis-Angelakis K (1999) The generation of active oxygen species differs in Nicotiana and Vitis plant protoplasts. Plant Physiol 121: 197-205.
81. Papadakis A, Paschalidis K, Roubelakis-Angelakis K (2005) Biosynthesis profile and endogenous titers of polyamines differ in totipotent and recalcitrant plant protoplasts. Physiol Plantarum 125: 10-20.
82. Pasternak T, Potters G, Caubergs R, Jansen M (2005) Complementary interactions between oxidative stress and auxins control plant growth responses at plant, organ, and cellular level. J Exp Biol 56: 1991-2001.
83. Patel D, Power J, Anthony P, Badakshi F, Heslop-Harrison J, Davey M (2011) Somatic hybrid plants of Nicotiana × sanderae + N. debneyi with fungal resistance to Peronospora tabacina. Ann Bot 108: 809-819.
84. Pati P, Sharma M, Ahuja P (2005) Extra thin alginate film: an efficient technique for protoplast culture. Protoplasma 226: 217-221.
85. Pati P, Sharma M, Ahuja P (2008) Rose protoplast isolation and culture and heterokaryon selection by immobilization in extra thin alginate film. Protoplasma 233: 165-171.
86. Petrivalsky M, Vanickova P, Ryzi M, Návratilová B, Piterkova J, Sedlarova M, Luhova L (2012) The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts. Plant Cell Tiss Org 108: 237-249.
87. Potters G, Jansen M, Horemans N, Guisez Y, Pasternak T (2010) Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts. In Vitro Cell Dev Plant 46: 289-297.
88. Prange A, Bartsch M, Serek M, Winkelmann T (2010a) Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts. Sci Hortic 125: 442-450.
89. Prange A, Serek M, Bartsch M, Winkelmann T (2010b) Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis. Plant Cell Tiss Org 101: 171-182.
90. Prange A, Bartsch M, Meiners J, Serek M, Winkelmann T (2012) Interspecific somatic hybrids between Cyclamen persicum and C. coum, two sexually incompatible species. Plant Cell Rep 31: 723-735.
91. Rakosy-Tican E, Aurori A, Vesa S, Kovacs K (2007) In vitro morphogenesis of sunflower (Helianthus annuus) hypocotyl protoplasts: the effects of protoplast density, haemoglobin and spermidine. Plant Cell Tiss Org 90: 55-62.
92. Riazunnisa K, Padmavathi L, Scheibe R, Raghavendra A (2007) Preparation of Arabidopsis mesophyll protoplasts with high rates of photosynthesis. Physiol Plantarum 129: 679-686.
93. Saito H, Nakano M (2002) Isolation and characterization of gametic microprotoplasts from developing microspores of Lilium longiflorum for partial genome transfer in the Liliaceous ornamentals. Sex Plant Reprod 15: 179-185.
94. Sarkar D, Tiwari J, Sharma S, Sharma Poonam S, Gopal J, Singh B, Luthra S, Pandey S, Pattanayak D (2011) Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun. Plant Cell Tiss Org 107: 427-440.
95. Scholze P, Kramer R, Ryschka U, Klocke E, Schumann G (2010) Somatic hybrids of vegetable brassicas as source for new resistances to fungal and virus diseases. Euphytica 176: 1-14.
96. Shan X, Ou X, Liu Z, Dong Y, Lin X, Li X, Liu B (2009) Transpositional activation of mPing in an asymmetric nuclear somatic cell hybrid of rice and Zizania latifolia was accompanied by massive element loss. Theor Appl Genet 119: 1325-1333.
97. Sheahan M, Rose R, McCurdy D (2004) Organelle inheritance in plant cell division: the actin cytoskeleton is required for unbiased inheritance of chloroplasts, mitochondria and endoplasmic reticulum in dividing protoplasts. Plant J 37: 379-390.
98. Sheahan M, McCurdy D, Rose R (2005) Mitochondria as a connected population: ensuring continuity of the mitochondrial genome during plant cell dedifferentiation through massive mitochondrial fusion. Plant J 44: 744-755.
99. Sheahan M, Rose R, McCurdy D (2007) Actin-filament-dependent remodeling of the vacuole in cultured mesophyll protoplasts. Protoplasma 230: 141-152.
100. Sheng X, Liu F, Zhu Y, Zhao H, Zhang L, Chen B (2008) Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana. Plant Cell Tiss Org 92: 55-62.
101. Sheng X, Zhao Z, Yu H, Wang J, Xiaohui Z, Gu H (2011) Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Plant Cell Tiss Org 107: 513-520.
102. Shiba T, Mii M (2005) Plant regeneration from mesophyll-and cell suspension-derived protoplasts of Dianthus acicularis and characterization of regenerated plants. In Vitro Cell Dev Plant 41: 794-800.
103. Shrestha B, Tokuhara K, Mii M (2007) Plant regeneration from cell suspension-derived protoplasts of Phalaenopsis. Plant Cell Rep 26: 719-725.
104. Soriano L, Assis F, Camargo L, Cristofani-Yaly M, Rocha R, Andrade C, Alves F, Januzzi B (2012) Regeneration and characterization of somatic hybrids combining sweet orange and mandarin/mandarin hybrid cultivars for citrus scion improvement. Plant Cell Tiss Org 111: 385-392.
105. Sun Y, Zhang X, Huang C, Nie Y, Guo X (2005a) Factors influencing in vitro regeneration from protoplasts of wild cotton (G. klotzschianum A) and RAPD analysis of regenerated plantlets. Plant Growth Regul 46: 79-86.
106. Sun Y, Zhang X, Nie Y, Guo X (2005b) Production of fertile somatic hybrids of Gossypium hirsutum plus G. bickii and G. hirsutum plus G. stockii via protoplast fusion. Plant Cell Tiss Org 83: 303-310.
107. Sytnik E, Komarnytsky I, Gleba Y, Kuchuk N (2005) Transfer of transformed chloroplasts from Nicotiana tabacum to the Lycium barbarum plants. Cell Biol Int 29: 71-75.
108. Szczerbakowa A, Tarwacka J, Oskiera M, Jakuczun H, Wielgat B (2010) Somatic hybridization between the diploids of S. michoacanum and S. tuberosum. Acta Physiol Plant 32: 867-873.
109. Szczerbakowa A, Tarwacka J, Sliwinska E, Wielgat B (2011) Nuclear DNA content and chromosome number in somatic hybrid allopolyploids of Solanum. Plant Cell Tiss Org 106: 373-380.
110. Takami K, Matsumaru A, Yahata M, Kunitake H, Komatsu H (2005) Utilization of intergeneric somatic hybrids as an index discriminating taxa in the genus Citrus and its related species. Sex Plant Reprod 18: 21-28.
111. Thieme R, Darsow U, Rakosy-Tican L, Kang Z, Gavrilenko T, Antonova O, Heimbach U, Thieme T (2004) Use of somatic hybridization to transfer resistance to late blight and potato virus Y (PVY) into cultivated potato. Plant Breed Seed Sci 50: 113-118.
112. Thieme R, Rakosy-Tican E, Nachtigall M, Schubert J, Hammann T, Antonova O, Gavrilenko T, Heimbach U, Thieme T (2010) Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Rep 29: 1187-1201.
113. Toppino L, Menella G, Rizza F, D'Alessandro A, Sihachakr D, Rotino G (2008) ISSR and isozyme characterization of androgenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between Solanum melongena and Solanum aethiopicum Group Gilo. J Hered 99: 304-315.
114. Trabelsi S, Gargouri-Bouzid R, Vedel F, Nato A, Lakhoua L, Drira N (2005) Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Plant Cell Tiss Org 83: 1-11.
115. Tu Y, Sun J, Liu Y, Ge X, Zhao Z, Yao X, Li Z (2008) Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica. Plant Cell Rep 27: 873-883.
116. Umate P, Rao K, Kiranmayee K, Jaya T, Sadanandam A (2005) Plant regeneration of mulberry (Morus indica) from mesophyll-derived protoplasts. Plant Cell Tiss Org 82: 289-293.
117. Wang M, Xia G, Peng Z (2005) High UV-tolerance with introgression hybrid formation of Bupleurum scorzonerifolium Willd. Plant Sci 168: 593-600.
118. Wang J, Sun Y, Yan S, Daud M, Zhu S (2008a) High frequency plant regeneration from protoplasts in cotton via somatic embryogenesis. Biol Plantarum 52: 616-620.
119. Wang M, Zhao J, Peng Z, Guo W, Wang Y, Wang L, Xia G (2008b) Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd. Plant Cell Tiss Org 92: 121-130.
120. Wang L, Pan Z, Guo W (2010) Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo. Plant Cell Tiss Org 103: 165-174.
121. Wang J, Zhao C, Liu C, Xia G, Xiang F (2011a) Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization. BioMedCentral Plant Biol 11: 71.
122. Wang M, Peng Z, Wang L, Zhao J, Che J, Xia G (2011b) Different rates of chromosome elimination in symmetric and asymmetric somatic hybridization between Festuca arundinacea and Bupleurum scorzonerifolium. Russ J Plant Physiol 58: 133-141.
123. Wang M, Peng Z, Wang M, Guo W, Zhao J, Zhi D, Xia G (2011c) The behavior of Bupleurum scorzonerifolium as a parent of somatic hybrid introgressed lines is associated with UV resistance of its chromosomes. Russ J Plant Physiol 58: 615-621.
124. Wang J, Jiang J, Wang Y (2013a) Protoplast fusion for crop improvement and breeding in China. Plant Cell Tiss Org 112: 131-142.
125. Wang X, Zhou J, Yang Y, Yu F, Chen J, Yu C, Wang F, Cheng Y, Yan C, Chen J (2013b) Transcriptome analysis of a progeny of somatic hybrids of cultivated rice (Oryza sativa L.) and wild rice (Oryza meyeriana L.) with high resistance to bacterial blight. J Phytopathol 161: 324-334.
126. Winkelmann T, Specht J, Serek M (2006) Efficient plant regeneration from protoplasts isolated from embryogenic suspension cultures of Cyclamen persicum Mill. Plant Cell Tiss Org 86: 337-347.
127. Wisniewska E, Majewska-Sawka A (2007) Arabinogalactan-proteins stimulate the organogenesis of guard cell protoplasts-derived callus in sugar beet. Plant Cell Rep 26: 1457-1467.
128. Wu S, Wirthensohn M, Hunt P, Gibson J, Sedgley M (2008) High resolution melting analysis of almond SNPs derived from ESTs. Theor Appl Genet 118: 1-14.
129. Wu F, Shen S, Lee L, Lee S, Chan M, Lin C (2009) Tape-Arabidopsis sandwich-a simpler Arabidopsis protoplast isolation method. Plant Meth 5: 16.
130. Xia G (2009) Progress of chromosome engineering mediated by asymmetric somatic hybridization. J Genet Genomics 36: 547-556.
131. Xiang F, Xia G, Zhi D, Wang J, Nie H, Chen M (2004) Regeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italic. Genome 47: 680-688.
132. Xiang F, Wang J, Xu C, Xia G (2010) The chromosome content and genotype of two wheat cell lines and of their somatic fusion product with oat. Planta 231: 1201-1210.
133. Xiao Z, Wan L, Han B (2004) An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance. Plant Cell Tiss Org 79: 299-306.
134. Xiao W, Huang X, Huang X, Chen Y, Dai X, Zhao J (2007) Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis. Plant Cell Tiss Org 90: 191-200.
135. Xu X, Liu J, Deng X (2005) FCM, SSR and CAPS analysis of intergeneric somatic hybrid plants between Changshou kumquat and Dancy tangerine. Bot Bull Acad Sinica 46: 93-98.
136. Xu X, Hu Z, Li J, Liu J, Deng X (2007) Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis: regeneration and characterization of hybrid shoots. Plant Cell Rep 26: 1263-1273.
137. Xu X, Xie G, He L, Zhang J, Xu X, Qian R, Liang G, Liu J (2013) Differences in oxidative stress, antioxidant systems, and microscopic analysis between regenerating callus-derived protoplasts and recalcitrant leaf mesophyll-derived protoplasts of Citrus reticulata Blanco. Plant Cell Tiss Org. 114: 161-169.
138. Yamagishi H, Nakagawa S, Kinoshita D, Ishibashi A, Yamashita Y (2008) Somatic hybrids between Arabidopsis thaliana and cabbage (Brassica oleracea L.) with all chromosomes derived from A. thaliana and low levels of fertile seed. J Jpn Soc Hortic Sci 77: 277-282.
139. Yang X, Guo X, Zhang X, Nie Y, Jin S (2007) Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis. Biol Plantarum 51: 533-537.
140. Yang X, Tu L, Zhu L, Fu L, Min L, Zhang X (2008) Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by suppression subtractive hybridization and macroarray. J Exp Bot 59: 3661-3674.
141. Yang Y, Guan S, Zhai H, He S, Liu Q (2009) Development and evaluation of a storage root-bearing sweet potato somatic hybrid between Ipomoea batatas (L.) Lam. and I. triloba L. Plant Cell Tiss Org 99: 83-89.
142. Yasuda K, Watanabe Y, Watanabe M (2007) Generation of intracellular reactive oxygen species during the isolation of Brassica napus leaf protoplasts. Plant Biotechnol 24: 361-366.
143. Yemets A, Blume Y (2009) Antimitotic drugs for microprotoplast-mediated chromosome transfer. In: Blume Y, Baird W, Yemets A, Breviario D (eds) The plant cytoskeleton: a key tool for agro-biotechnology. Springer, Dordrecht, pp 419-434.
144. Yu X, Chu B, Liu R, Sun J, Brian J, Wang H, Shuijin Z, Sun Y (2012) Characteristics of fertile somatic hybrids of G. hirsutum L. and G. trilobum generated via protoplast fusion. Theor Appl Genet 125: 1503-1516.
145. Zaban B, Maisch J, Nick P (2013) Dynamic actin controls polarity induction de novo in protoplasts. J Integr Plant Biol 55: 142-159.
146. Zhou A, Xia G (2005) Introgression of the Haynaldia villosa genome into gamma-ray-induced asymmetric somatic hybrids of wheat. Plant Cell Rep 24: 289-296.