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Volumn 108, Issue 2, 2012, Pages 237-249

The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts

Author keywords

Cucumis sativus; Nitric oxide; Protoplast; Reactive oxygen species; Regeneration

Indexed keywords

CELL DENSITY; CELL DIVISIONS; CELL VIABILITY; CELLULAR GROWTH; CUCUMIS SATIVUS; CULTURE MEDIUM; MESOPHYLL PROTOPLAST; NO DONOR; PROTOPLAST; PROTOPLAST ISOLATION; REACTIVE NITROGEN AND OXYGEN SPECIES; REACTIVE NITROGEN SPECIES; REACTIVE OXYGEN SPECIES; REGENERATION; S-NITROSOGLUTATHIONE; SODIUM NITROPRUSSIDE;

EID: 84856018030     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-011-0035-3     Document Type: Article
Times cited : (19)

References (55)
  • 1
    • 79957447361 scopus 로고    scopus 로고
    • Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip
    • Abbasi BH, Khan M, Guo B, Bokhari SA, Khan MA (2011) Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip. Plant Cell Tiss Org Cult 105: 337-344.
    • (2011) Plant Cell Tiss Org Cult , vol.105 , pp. 337-344
    • Abbasi, B.H.1    Khan, M.2    Guo, B.3    Bokhari, S.A.4    Khan, M.A.5
  • 2
    • 0031430355 scopus 로고    scopus 로고
    • Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells
    • Allan AC, Fluhr R (1997) Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells. Plant Cell 9: 1559-1572.
    • (1997) Plant Cell , vol.9 , pp. 1559-1572
    • Allan, A.C.1    Fluhr, R.2
  • 3
    • 0037008193 scopus 로고    scopus 로고
    • Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers
    • Beligni MV, Fath A, Bethke PC, Lamattina L, Jones RL (2002) Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Plant Physiol 129: 1642-1650.
    • (2002) Plant Physiol , vol.129 , pp. 1642-1650
    • Beligni, M.V.1    Fath, A.2    Bethke, P.C.3    Lamattina, L.4    Jones, R.L.5
  • 4
    • 0030772856 scopus 로고    scopus 로고
    • Protoplast regeneration from gametophytes and sporophytes of some species in the order Laminariales (Phaeophyceae)
    • Benet H, Ar Gall E, Asensi A, Kloareg B (1997) Protoplast regeneration from gametophytes and sporophytes of some species in the order Laminariales (Phaeophyceae). Protoplasma 199: 39-48.
    • (1997) Protoplasma , vol.199 , pp. 39-48
    • Benet, H.1    Ar Gall, E.2    Asensi, A.3    Kloareg, B.4
  • 6
    • 0028837153 scopus 로고
    • In vitro culture of Cucumis sativus L. XVIII. Plants from protoplasts through direct somatic embryogenesis
    • Burza W, Malepszy S (1995) In vitro culture of Cucumis sativus L. XVIII. Plants from protoplasts through direct somatic embryogenesis. Plant Cell Tiss Organ Cult 41: 259-266.
    • (1995) Plant Cell Tiss Organ Cult , vol.41 , pp. 259-266
    • Burza, W.1    Malepszy, S.2
  • 7
    • 84988632629 scopus 로고
    • Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L.). II. Natural fluorescence and direct somatic embryogenesis from protoplasts
    • Burza W, Wozniak B, Tarkowska JA, Malepszy S (1994) Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L.). II. Natural fluorescence and direct somatic embryogenesis from protoplasts. Acta Soc Bot Poloniae 63: 265-268.
    • (1994) Acta Soc Bot Poloniae , vol.63 , pp. 265-268
    • Burza, W.1    Wozniak, B.2    Tarkowska, J.A.3    Malepszy, S.4
  • 8
    • 0034973217 scopus 로고    scopus 로고
    • Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers
    • Cassells AC, Curry RF (2011) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Organ Cult 64: 145-157.
    • (2011) Plant Cell Tiss Organ Cult , vol.64 , pp. 145-157
    • Cassells, A.C.1    Curry, R.F.2
  • 9
    • 0031423791 scopus 로고    scopus 로고
    • Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive ritrogen and oxygen species
    • Crow JP (1997) Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive ritrogen and oxygen species. Nitric Oxide 1: 145-157.
    • (1997) Nitric Oxide , vol.1 , pp. 145-157
    • Crow, J.P.1
  • 10
    • 84856020567 scopus 로고
    • Selective methods for the recovery of somatic hybrids of Cucumis melo × metuliferus and C. sativus × C. metuliferus
    • Dabauza M, Roig LA, Moreno V (1991) Selective methods for the recovery of somatic hybrids of Cucumis melo × metuliferus and C. sativus × C. metuliferus. Cucurbit Gen Coop Rep 14: 81-84.
    • (1991) Cucurbit Gen Coop Rep , vol.14 , pp. 81-84
    • Dabauza, M.1    Roig, L.A.2    Moreno, V.3
  • 11
    • 20644432459 scopus 로고    scopus 로고
    • Plant protoplasts: status and biotechnological perspectives
    • Davey MR, Anthony P, Power JB, Lowe KC (2005) Plant protoplasts: status and biotechnological perspectives. Biotech Adv 23: 131-171.
    • (2005) Biotech Adv , vol.23 , pp. 131-171
    • Davey, M.R.1    Anthony, P.2    Power, J.B.3    Lowe, K.C.4
  • 12
    • 0030024328 scopus 로고    scopus 로고
    • The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts
    • De Marco A, Roubelakis-Angelakis KA (1996) The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol 110: 137-145.
    • (1996) Plant Physiol , vol.110 , pp. 137-145
    • de Marco, A.1    Roubelakis-Angelakis, K.A.2
  • 13
    • 0002818269 scopus 로고
    • Induction of static embryogenesis and callogenesis from cotyledons and leaf protoplasts-derived colonies of melon (Cucumis melo L.)
    • Debeaujon I, Branchard M (1992) Induction of static embryogenesis and callogenesis from cotyledons and leaf protoplasts-derived colonies of melon (Cucumis melo L.). Plant Cell Rep 12: 37-40.
    • (1992) Plant Cell Rep , vol.12 , pp. 37-40
    • Debeaujon, I.1    Branchard, M.2
  • 14
    • 1842716716 scopus 로고    scopus 로고
    • Nitric oxide and nitric oxide synthase activity in plants
    • Del Rio LA, Corpas FJ, Barroso JB (2004) Nitric oxide and nitric oxide synthase activity in plants. Phytochemistry 65: 783-792.
    • (2004) Phytochemistry , vol.65 , pp. 783-792
    • Del Rio, L.A.1    Corpas, F.J.2    Barroso, J.B.3
  • 15
    • 0035818606 scopus 로고    scopus 로고
    • Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response
    • Delledonne M, Zeier J, Marocco A, Lamb C (2001) Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proc Natl Acad Sci USA 98: 13454-13459.
    • (2001) Proc Natl Acad Sci USA , vol.98 , pp. 13454-13459
    • Delledonne, M.1    Zeier, J.2    Marocco, A.3    Lamb, C.4
  • 16
    • 0032410993 scopus 로고    scopus 로고
    • Cyclus induction and protoplast isolation from tissues of Cucumis sativus L. and C. melo L. seedlings
    • Fellner M, Lebeda A (1998) Cyclus induction and protoplast isolation from tissues of Cucumis sativus L. and C. melo L. seedlings. Biol Plantarum 41: 11-24.
    • (1998) Biol Plantarum , vol.41 , pp. 11-24
    • Fellner, M.1    Lebeda, A.2
  • 19
    • 34548287863 scopus 로고    scopus 로고
    • Effects of genotype, source tissue and media composition on Cucumis and Cucurbita protoplast isolation and regeneration
    • Gajdová J, Navrátilová B, Smolná I, Lebeda A (2007a) Effects of genotype, source tissue and media composition on Cucumis and Cucurbita protoplast isolation and regeneration. Acta Hortic 731: 89-94.
    • (2007) Acta Hortic , vol.731 , pp. 89-94
    • Gajdová, J.1    Navrátilová, B.2    Smolná, I.3    Lebeda, A.4
  • 21
    • 57049106803 scopus 로고    scopus 로고
    • Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets
    • Han X, Yang H, Duan K, Zhang X, Zhao H, You S, Jiang Q (2009) Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. Plant Cell Tiss Organ Cult 96: 29-34.
    • (2009) Plant Cell Tiss Organ Cult , vol.96 , pp. 29-34
    • Han, X.1    Yang, H.2    Duan, K.3    Zhang, X.4    Zhao, H.5    You, S.6    Jiang, Q.7
  • 22
    • 63949087268 scopus 로고    scopus 로고
    • Role of nitric oxide in UV-B-induced activation of PAL and stimulation of flavonoid biosynthesis in Ginkgo biloba callus
    • Hao G, Du X, Zhao F, Shi R, Wang J (2009) Role of nitric oxide in UV-B-induced activation of PAL and stimulation of flavonoid biosynthesis in Ginkgo biloba callus. Plant Cell Tiss Organ Cult 97: 175-185.
    • (2009) Plant Cell Tiss Organ Cult , vol.97 , pp. 175-185
    • Hao, G.1    Du, X.2    Zhao, F.3    Shi, R.4    Wang, J.5
  • 23
    • 0014238667 scopus 로고
    • Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds
    • Harrington BJ, Raper KB (1968) Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds. Appl Microbiol 16: 106-113.
    • (1968) Appl Microbiol , vol.16 , pp. 106-113
    • Harrington, B.J.1    Raper, K.B.2
  • 24
    • 0002176044 scopus 로고
    • Factors influencing protoplast viability of suspension-cultured rice cells during isolation process
    • Ishii S (1988) Factors influencing protoplast viability of suspension-cultured rice cells during isolation process. Plant Physiol 88: 26-29.
    • (1988) Plant Physiol , vol.88 , pp. 26-29
    • Ishii, S.1
  • 25
    • 78149498879 scopus 로고    scopus 로고
    • Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L
    • Kalra C, Babbar SB (2010) Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L. Plant Cell Tiss Organ Cult 103: 353-359.
    • (2010) Plant Cell Tiss Organ Cult , vol.103 , pp. 353-359
    • Kalra, C.1    Babbar, S.B.2
  • 26
    • 33645820618 scopus 로고    scopus 로고
    • Iron released by sodium nitroprusside contributes to heme oxygenase-1 induction via the cAMP-protein kinase A-mitogen-activated protein kinase pathway in RAW 264.7 cells
    • Kim HJ, Tsoy I, Park MK, Lee YS, Lee JH, Seo HG, Chang KC (2006) Iron released by sodium nitroprusside contributes to heme oxygenase-1 induction via the cAMP-protein kinase A-mitogen-activated protein kinase pathway in RAW 264. 7 cells. Mol Pharmacol 69: 1633-1640.
    • (2006) Mol Pharmacol , vol.69 , pp. 1633-1640
    • Kim, H.J.1    Tsoy, I.2    Park, M.K.3    Lee, Y.S.4    Lee, J.H.5    Seo, H.G.6    Chang, K.C.7
  • 27
    • 11144250304 scopus 로고    scopus 로고
    • The role of nitric oxide in plant growth regulation and responses to abiotic stresses
    • Kopyra M, Gwozdz EA (2004) The role of nitric oxide in plant growth regulation and responses to abiotic stresses. Acta Physiol Plantarm 26: 459-472.
    • (2004) Acta Physiol Plantarm , vol.26 , pp. 459-472
    • Kopyra, M.1    Gwozdz, E.A.2
  • 28
    • 0141721194 scopus 로고    scopus 로고
    • Pseudoperonospora cubensis on Cucumis spp. and Cucurbita spp.-resistance breeding aspects
    • Lebeda A (1999) Pseudoperonospora cubensis on Cucumis spp. and Cucurbita spp.-resistance breeding aspects. Acta Hortic 492: 363-370.
    • (1999) Acta Hortic , vol.492 , pp. 363-370
    • Lebeda, A.1
  • 29
    • 54549119032 scopus 로고    scopus 로고
    • Timing is everything: regulatory overlap in plant cell death
    • Love AJ, Miller JJ, Sadanandom A (2008) Timing is everything: regulatory overlap in plant cell death. Trends Plant Sci 13: 585-595.
    • (2008) Trends Plant Sci , vol.13 , pp. 585-595
    • Love, A.J.1    Miller, J.J.2    Sadanandom, A.3
  • 30
    • 84982358134 scopus 로고
    • A revised medium for rapid growth and bioassays with tobacco tissue cultures
    • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
    • (1962) Physiol Plant , vol.15 , pp. 473-497
    • Murashige, T.1    Skoog, F.2
  • 32
    • 85047684872 scopus 로고    scopus 로고
    • Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells
    • Neill SJ, Desikan R, Clarke A, Hancock JT (2002) Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells. Plant Physiol 128: 13-16.
    • (2002) Plant Physiol , vol.128 , pp. 13-16
    • Neill, S.J.1    Desikan, R.2    Clarke, A.3    Hancock, J.T.4
  • 34
    • 58149195152 scopus 로고    scopus 로고
    • The heterochromatin as a marker for protoplast differentiation of Cucumis sativus
    • Ondřej V, Navrátilová B, Lebeda A (2009) The heterochromatin as a marker for protoplast differentiation of Cucumis sativus. Plant Cell Tiss Organ Cult 96: 229-234.
    • (2009) Plant Cell Tiss Organ Cult , vol.96 , pp. 229-234
    • Ondřej, V.1    Navrátilová, B.2    Lebeda, A.3
  • 36
    • 0000927488 scopus 로고
    • In vitro culture of Cucumis sativus L. V. Stabilizing effect of glycine on leaf protoplasts
    • Orczyk W, Malepszy S (1985) In vitro culture of Cucumis sativus L. V. Stabilizing effect of glycine on leaf protoplasts. Plant Cell Rep 4: 269-273.
    • (1985) Plant Cell Rep , vol.4 , pp. 269-273
    • Orczyk, W.1    Malepszy, S.2
  • 37
    • 31644443156 scopus 로고    scopus 로고
    • Nitric oxide is required for, and promotes auxin-mediated activation of, cell division and embryogenic cell formation but does not influence cell cycle progression in alfalfa cell cultures
    • Ötvös K, Pasternak TP, Miskolczi P, Domoki M, Dorjgotov D, Szücs A, Bottka S, Dudits D, Fehér A (2005) Nitric oxide is required for, and promotes auxin-mediated activation of, cell division and embryogenic cell formation but does not influence cell cycle progression in alfalfa cell cultures. Plant J 43: 849-860.
    • (2005) Plant J , vol.43 , pp. 849-860
    • Ötvös, K.1    Pasternak, T.P.2    Miskolczi, P.3    Domoki, M.4    Dorjgotov, D.5    Szücs, A.6    Bottka, S.7    Dudits, D.8    Fehér, A.9
  • 38
    • 0033198494 scopus 로고    scopus 로고
    • The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts
    • Papadakis AK, Roubelakis-Angelakis KA (1999) The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts. Plant Physiol 121: 197-206.
    • (1999) Plant Physiol , vol.121 , pp. 197-206
    • Papadakis, A.K.1    Roubelakis-Angelakis, K.A.2
  • 39
    • 0034973675 scopus 로고    scopus 로고
    • Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts
    • Papadakis AK, Siminis CI, Roubelakis-Angelakis KA (2001) Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts. Plant Physiol 126: 434-444.
    • (2001) Plant Physiol , vol.126 , pp. 434-444
    • Papadakis, A.K.1    Siminis, C.I.2    Roubelakis-Angelakis, K.A.3
  • 40
    • 24944462436 scopus 로고    scopus 로고
    • Complementary interactions between oxidative stress and auxins control plant growth responses at plant, organ, and cellular level
    • Pasternak TP, Potters G, Caubergs R, Jansen MAK (2005) Complementary interactions between oxidative stress and auxins control plant growth responses at plant, organ, and cellular level. J Exp Bot 56: 1991-2001.
    • (2005) J Exp Bot , vol.56 , pp. 1991-2001
    • Pasternak, T.P.1    Potters, G.2    Caubergs, R.3    Jansen, M.A.K.4
  • 42
    • 0031664744 scopus 로고    scopus 로고
    • The relationship between the regeneration system and genetic variability in the cucumber (Cucumis sativus L)
    • Plader W, Malpszy S, Burza W, Rusinowski Z (1998) The relationship between the regeneration system and genetic variability in the cucumber (Cucumis sativus L). Euphytica 103: 9-15.
    • (1998) Euphytica , vol.103 , pp. 9-15
    • Plader, W.1    Malpszy, S.2    Burza, W.3    Rusinowski, Z.4
  • 43
    • 33745646568 scopus 로고    scopus 로고
    • Production of reactive oxygen species by plant NADPH oxidases
    • Sagi M, Fluhr R (2006) Production of reactive oxygen species by plant NADPH oxidases. Plant Physiol 141: 336-340.
    • (2006) Plant Physiol , vol.141 , pp. 336-340
    • Sagi, M.1    Fluhr, R.2
  • 44
    • 85047682075 scopus 로고    scopus 로고
    • Signal transduction in maize and Arabidopsis mesophyll protoplasts
    • Sheen J (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol 127: 1466-1475.
    • (2001) Plant Physiol , vol.127 , pp. 1466-1475
    • Sheen, J.1
  • 45
    • 0028002822 scopus 로고
    • Catalase is differentially expressed in dividing and nondividing protoplasts
    • Siminis CI, Kanellis AK, Roubelakis-Angelakis KA (1994) Catalase is differentially expressed in dividing and nondividing protoplasts. Plant Physiol 105: 1375-1383.
    • (1994) Plant Physiol , vol.105 , pp. 1375-1383
    • Siminis, C.I.1    Kanellis, A.K.2    Roubelakis-Angelakis, K.A.3
  • 46
    • 78649365890 scopus 로고    scopus 로고
    • Optimizing culture for in vitro pollination and fertilization in Cucumis sativus and C. melo
    • Skálová D, Navrátilová B, Ondřej V, Lebeda A (2010) Optimizing culture for in vitro pollination and fertilization in Cucumis sativus and C. melo. Acta Biol Cracov 52: 111-115.
    • (2010) Acta Biol Cracov , vol.52 , pp. 111-115
    • Skálová, D.1    Navrátilová, B.2    Ondřej, V.3    Lebeda, A.4
  • 47
    • 33746633396 scopus 로고    scopus 로고
    • Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed
    • Song L, Ding W, Zhao MG, Sun B, Zhang L (2006) Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed. Plant Sci 171: 449-458.
    • (2006) Plant Sci , vol.171 , pp. 449-458
    • Song, L.1    Ding, W.2    Zhao, M.G.3    Sun, B.4    Zhang, L.5
  • 48
    • 54249127295 scopus 로고    scopus 로고
    • Nitric oxide mediates abscisic acid induced thermotolerance in the calluses from two ecotypes of reed under heat stress
    • Song L, Ding W, Shen J, Zhang Z, Bi Y, Zhang L (2008) Nitric oxide mediates abscisic acid induced thermotolerance in the calluses from two ecotypes of reed under heat stress. Plant Sci 175: 826-832.
    • (2008) Plant Sci , vol.175 , pp. 826-832
    • Song, L.1    Ding, W.2    Shen, J.3    Zhang, Z.4    Bi, Y.5    Zhang, L.6
  • 51
    • 33645544479 scopus 로고    scopus 로고
    • Calcium-mediated mitochondrial permeability transition involved in hydrogen peroxide-induced apoptosis in tobacco protoplasts
    • Wang Y, Lin JS, Wang GX (2006) Calcium-mediated mitochondrial permeability transition involved in hydrogen peroxide-induced apoptosis in tobacco protoplasts. J Integ Plant Biol 48: 433-439.
    • (2006) J Integ Plant Biol , vol.48 , pp. 433-439
    • Wang, Y.1    Lin, J.S.2    Wang, G.X.3
  • 52
    • 70350582776 scopus 로고    scopus 로고
    • Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita
    • Xu J, Yin H, Wang W, Mi Q, Liu X (2009) Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita. Plant Growth Regul 59: 279-285.
    • (2009) Plant Growth Regul , vol.59 , pp. 279-285
    • Xu, J.1    Yin, H.2    Wang, W.3    Mi, Q.4    Liu, X.5
  • 53
    • 79952694702 scopus 로고    scopus 로고
    • 2 signaling in PB90-induced hypericin accumulation in Hypericum perforatum cells
    • 2 signaling in PB90-induced hypericin accumulation in Hypericum perforatum cells. Plant Cell Tiss Organ Cult 105: 47-53.
    • (2011) Plant Cell Tiss Organ Cult , vol.105 , pp. 47-53
    • Xu, M.1    Sheng, J.2    Wang, H.3    Dong, J.4
  • 54
    • 33745449719 scopus 로고    scopus 로고
    • Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death
    • Yao N, Greenberg JT (2006) Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death. Plant Cell 18: 397-411.
    • (2006) Plant Cell , vol.18 , pp. 397-411
    • Yao, N.1    Greenberg, J.T.2
  • 55
    • 34548825673 scopus 로고    scopus 로고
    • Generation of intracellular reactive oxygen species during the generation of intracellular reactive oxygen species during the isolation of Brassica napus leaf protoplasts
    • Yasuda K, Watanabe M (2007) Generation of intracellular reactive oxygen species during the generation of intracellular reactive oxygen species during the isolation of Brassica napus leaf protoplasts. Plant Biotech 24: 361-366.
    • (2007) Plant Biotech , vol.24 , pp. 361-366
    • Yasuda, K.1    Watanabe, M.2


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