The bacterial Entner-Doudoroff pathway does not replace glycolysis in Saccharomyces cerevisiae due to the lack of activity of iron-sulfur cluster enzyme 6-phosphogluconate dehydratase

Journal of Biotechnology

Volumn 171, Issue 1, 2014, Pages 45-55

  Benisch, Feline ^a   Boles, Eckhard ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Entner Doudoroff pathway; Iron sulfur cluster; KDPG aldolase; Metabolic engineering; Product yield; Saccharomyces cerevisiae

Indexed keywords

ALDOLASES; BIOCHEMICAL PATHWAY; ENTNER-DOUDOROFF PATHWAYS; FUNCTIONAL EXPRESSION; GLYCOLYTIC PATHWAYS; IRON-SULFUR CLUSTERS; PENTOSE PHOSPHATE PATHWAY; PRODUCT YIELDS;

ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; IRON; MACHINERY; METABOLIC ENGINEERING; PATHOLOGY; PROTEINS; SULFUR;

YEAST;

2 DEHYDRO 3 DEOXY DEXTRO GLUCONATE 6 PHOSPHATE; BACTERIAL ENZYME; CELL EXTRACT; GLUCONIC ACID; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLYCOLYTIC ENZYME; HYBRID PROTEIN; IRON; IRON SULFUR PROTEIN; ISOLEUCINE; LEUCINE; MITOCHONDRIAL PROTEIN; PHOSPHOGLUCONATE DEHYDRATASE; PHOSPHOGLUCONATE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; VALINE;

ARTICLE; BIOAVAILABILITY; CELL DISRUPTION; CELLULAR DISTRIBUTION; CODON; CONTROLLED STUDY; DELETION MUTANT; DOWN REGULATION; ENTNER DOUDOROFF PATHWAY; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; FUNGAL MEMBRANE; FUNGAL STRAIN; FUNGUS GROWTH; GENE DELETION; GENE TRANSLOCATION; GROWTH INHIBITION; IMMUNOFLUORESCENCE MICROSCOPY; IRON TRANSPORT; MITOCHONDRIAL TARGETING SIGNAL; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; UPREGULATION; WESTERN BLOTTING; WILD TYPE; YEAST CELL;

ENTNER-DOUDOROFF PATHWAY; IRON-SULFUR CLUSTER; KDPG ALDOLASE; METABOLIC ENGINEERING; PRODUCT YIELD; SACCHAROMYCES CEREVISIAE;

CODON; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLYCOLYSIS; HYDRO-LYASES; IRON-SULFUR PROTEINS; METABOLIC NETWORKS AND PATHWAYS; SACCHAROMYCES CEREVISIAE;

EID: 84890895919     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.025     Document Type: Article

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