Detection of small interfering RNA (siRNA) by mass spectrometry procedures in doping controls

Drug Testing and Analysis

Volumn 5, Issue 11-12, 2013, Pages 853-860

  Thomas, Andreas ^a   Walpurgis, Katja ^a   Delahaut, Philippe ^b   Kohler, Maxie ^c   Schänzer, Wilhelm ^a   Thevis, Mario ^a  

^a GERMAN SPORT UNIVERSITY COLOGNE   (Germany)

^b 8 ^*  (Belgium)

^c UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Genetic doping; Mass spectrometry; Sport; Urine

Indexed keywords

ALKYLATION; BLOOD; CONTROLLED DRUG DELIVERY; DRUG PRODUCTS; GENES; HYDROLYSIS; LIQUID CHROMATOGRAPHY; OLIGONUCLEOTIDES; RNA; SPORTS;

BLOOD SAMPLES; DOPING CONTROL; GENETIC DOPING; HIGH RESOLUTION; HIGH-ACCURACY; MODIFIED NUCLEOTIDES; REALISTIC SCENARIO; SMALL INTERFERING RNA; TARGET ANALYTES; URINE;

MASS SPECTROMETRY;

CHOLESTEROL; LOCKED NUCLEIC ACID; MESSENGER RNA; MYOSTATIN; NUCLEOTIDE DERIVATIVE; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ARTICLE; ATHLETIC PERFORMANCE; CONTROLLED STUDY; DOPING; DRUG SCREENING; FEMALE; GENE IDENTIFICATION; GENE TARGETING; GENETIC CODE; HYDROLYSIS; IN VIVO STUDY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITE; METHYLATION; MUSCLE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RAT; RATTUS NORVEGICUS; RNA INTERFERENCE; RNA PURIFICATION; URINALYSIS;

GENETIC DOPING; MASS SPECTROMETRY; SPORT; URINE;

ANIMALS; CHROMATOGRAPHY, LIQUID; DOPING IN SPORTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; MASS SPECTROMETRY; RATS; RATS, WISTAR; RNA, SMALL INTERFERING; SENSITIVITY AND SPECIFICITY; SUBSTANCE ABUSE DETECTION;

EID: 84890791479     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.1519     Document Type: Article

References (29)

1. A.C. McGinnis, B. Chen, M.G. Bartlett Chromatographic methods for the determination of therapeutic oligonucleotides. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2012, 883, 76.
2. M.A. Behlke Chemical modification of siRNAs for in vivo use. Oligonucleotides 2008, 18, 305.
3. D. Bumcrot, M. Manoharan, V. Koteliansky, D.W. Sah RNAi therapeutics: A potential new class of pharmaceutical drugs. Nat. Chem. Biol. 2006, 2, 711.
4. J. Kurreck RNA interference: from basic research to therapeutic applications. Angew. Chem. Int. Ed. Engl. 2009, 48, 1378.
5. D. Haussecker The business of RNAi therapeutics. Hum. Gene Ther. 2008, 19, 451.
6. K.A. Whitehead, R. Langer, D.G. Anderson Knocking down barriers: Advances in siRNA delivery. Nat. Rev. Drug Discov. 2009, 8, 129.
7. R. Juliano, J. Bauman, H. Kang, X. Ming Biological barriers to therapy with antisense and siRNA oligonucleotides. Mol. Pharmaceut. 2009, 6, 686.
8. J. Kurreck Antisense technologies. Improvement through novel chemical modifications. Eur. J. Biochem. 2003, 270, 1628.
9. WADA. List of prohited stubstances and methords. Available at: http://www.wada-ama.org/en/Science-Medicine/Prohibited-List/ [02 May 2013].
10. M. Schuelke, K.R. Wagner, L.E. Stolz, C. Hubner, T. Riebel, W. Komen, T. Braun, J. F. Tobin, S. J. Lee Myostatin mutation associated with gross muscle hypertrophy in a child. New Engl. J. Med. 2004, 350, 2682.
11. C.M. Liu, Z. Yang, C.W. Liu, R. Wang, P. Tien, R. Dale, et al. Myostatin antisense RNA-mediated muscle growth in normal and cancer cachexia mice. Gene Ther. 2008, 15, 155.
12. P. Chaltin, A. Margineanu, D. Marchand, A. Van Aerschot, J. Rozenski, F. De Schryver, A. Herrmann, K. Mullen, R. Juliano, M. H. Fisher, H. Kang, S. De Feyter, P. Herdewijn Delivery of antisense oligonucleotides using cholesterol-modified sense dendrimers and cationic lipids. Bioconjug. Chem. 2005, 16, 827.
13. B. Chen, M. Bartlett A one-step solid phase extraction method for bioanalysis of a phosphorothioate oligonucleotide and its 3' n-1 metabolite from rat plasma by uHPLC-MS/MS. AAPS J. 2012, 14, 772.
14. B. Chen, M.G. Bartlett Determination of therapeutic oligonucleotides using capillary gel electrophoresis. Biomed. Chromatogr. 2012, 26, 409.
15. R. Erb, K. Leithner, A. Bernkop-Schnurch, H. Oberacher Phosphorothioate oligonucleotide quantification by mu-liquid chromatography-mass spectrometry. AAPS J. 2012, 14, 728.
16. M. Gilar Analysis and purification of synthetic oligonucleotides by reversed-phase high-performance liquid chromatography with photodiode array and mass spectrometry detection. Anal. Biochem. 2001, 298, 196.
17. C.G. Huber, H. Oberacher Analysis of nucleic acids by on-line liquid chromatography-mass spectrometry. Mass Spectrom. Rev. 2001, 20, 310.
18. H. Rutters, T. Mohring, J. Rullkotter, J. Griep-Raming, J.O. Metzger The persistent memory effect of triethylamine in the analysis of phospholipids by liquid chromatography/mass spectrometry. Rapid Commun. Mass Spectrom. 2000, 14, 122.
19. K. Bleicher, E. Bayer Analysis of Oligonucleotides using coupled high performance liquid chromatography-electrospray mass spectrometry. Chromatographia 1994, 39, 405.
20. P.A. Limbach Indirect mass spectrometric methods for characterizing and sequnencing oligonucleotides. Mass Spectrom. Rev. 1996, 15, 297.
21. U. Bahr, H. Aygun, M. Karas Sequencing of single and double stranded RNA oligonucleotides by acid hydrolysis and MALDI mass spectrometry. Anal. Chem. 2009, 81, 3173.
22. M. Taoka, M. Ikumi, H. Nakayama, S. Masaki, R. Matsuda, Y. Nobe, et al. In-gel digestion for mass spectrometric characterization of RNA from fluorescently stained polyacrylamide gels. Anal. Chem. 2010, 82, 7795.
23. M. Taoka, Y. Yamauchi, Y. Nobe, S. Masaki, H. Nakayama, H. Ishikawa, N. Takahashi, T. Isobe An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. Nucleic Acids Res. 2009, 37, e140.
24. K. Ui-Tei, Y. Naito, F. Takahashi, T. Haraguchi, H. Ohki-Hamazaki, A. Juni, R. Ueda, K. Saigo Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference. Nucleic Acids Res. 2004, 32, 936.
25. NCBI. Rattus norvegicus myostatin (Mstn), mRNA. Available at: http://www.ncbi.nlm.nih.gov/nuccore/NM_019151.1 [13 May 2007].
26. Ambion. RNA reagents and consumables. Available at: http://www.ambion.com/techlib/misc/siRNA_converter.html [11 May 2012].
27. M. Kohler, A. Thomas, K. Walpurgis, W. Schänzer, M. Thevis Mass spectrometric detection of siRNA in plasma samples for doping control purposes. Anal. Bioanal. Chem. 2010, 398, 1305.
28. M. Kohler, W. Schänzer, M. Thevis RNA interference for performance enhancement and detection in doping control. Drug Test. Anal. 2011, 3, 661.
29. S.A. McLuckey, G.J. Van Berkel, G.L. Glish Tandem mass spectrometry of small, multiply charged oligonucleotides. J. Am. Soc. Mass Spectrom. 1992, 3, 60.