Extracellular l-asparaginase from a protease-deficient bacillus aryabhattai ITBHU02: Purification, biochemical characterization, and evaluation of antineoplastic activity in vitro

Applied Biochemistry and Biotechnology

Volumn 171, Issue 7, 2013, Pages 1759-1774

  Singh, Yogendra ^a   Gundampati, Ravi Kumar ^b   Jagannadham, Medicherla V ^b   Srivastava, S K ^a  

^a BANARAS HINDU UNIVERSITY   (India)

^b BANARAS HINDU UNIVERSITY   (India)

Author keywords

Antileukemic activity; Bacillus aryabhattai ITBHU02; Biochemical characterization; Circular dichroism; l Asparaginase; N terminal sequence

Indexed keywords

AMMONIUM SULFATE; ANTINEOPLASTIC ACTIVITY; BACILLUS MEGATERIUM; BIOCHEMICAL CHARACTERIZATION; DIETHYLAMINOETHYL; L-ASPARAGINASE; N-TERMINAL SEQUENCES; SPECIFIC ACTIVITY;

BACTERIOLOGY; CELL CULTURE; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; ENZYMES;

PURIFICATION;

ASPARAGINASE;

AMINO TERMINAL SEQUENCE; ANTINEOPLASTIC ACTIVITY; ARTICLE; BACILLUS; BACILLUS ARYABHATTAI; BACILLUS MEGATERIUM; BACTERIAL STRAIN; CIRCULAR DICHROISM; CONTROLLED STUDY; CYTOTOXICITY ASSAY; DRUG PURIFICATION; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL FILTRATION; IN VITRO STUDY; LEUKEMIA CELL; MOLECULAR WEIGHT; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACILLUS MEGATERIUM;

ANTINEOPLASTIC AGENTS; ASPARAGINASE; BACILLUS; CELL LINE, TUMOR; ENZYME STABILITY; EXTRACELLULAR SPACE; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; PEPTIDE HYDROLASES; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84890569631     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-013-0455-0     Document Type: Article

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