Human parenchymal and non-parenchymal liver cell isolation, culture and characterization

Hepatology International

Volumn 7, Issue 4, 2013, Pages 951-958

  Damm, Georg ^a   Pfeiffer, Elisa ^a   Burkhardt, Britta ^b   Vermehren, Jan ^c   Nüssler, Andreas K ^b   Weiss, Thomas S ^c  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c UNIVERSITY HOSPITAL REGENSBURG   (Germany)

Author keywords

Human hepatocytes; Liver cell culture; Liver cell isolation; Non parenchymal liver cells; Parenchymal liver cells

Indexed keywords

ANALYTIC METHOD; ARTICLE; CELL ACTIVATION; CELL DENSITY; CELL FUNCTION; CELL ISOLATION; CELL SIZE; CELL STRUCTURE; CLINICAL PROTOCOL; DENSITY GRADIENT CENTRIFUGATION; ENDOTHELIUM CELL; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; ISOLATED LIVER; KUPFFER CELL; LABORATORY TEST; LIVER CELL; LIVER CELL CULTURE; LIVER FUNCTION; LIVER PARENCHYMA; LIVER SINUSOID; MEDICAL RESEARCH; NONPARENCHYMAL LIVER CELL; PRIORITY JOURNAL; PURIFICATION; QUALITY CONTROL; STELLATE CELL;

EID: 84890505151     PISSN: 19360533     EISSN: 19360541     Source Type: Journal    
DOI: 10.1007/s12072-013-9475-7     Document Type: Article

References (39)

1. Alpini G, Philips JO, Vroman B, LaRusso NF. Recent advances in the isolation of liver cells. Hepatology 1994;20(2):494-514
2. Friedman SL, Roll FJ. Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal, endothelial cells by density gradient centrifugation with Stractan. Anal Biochem 1987;161(1):207-218
3. Bojar H, Basler M, Fuchs F, Dreyfürst R, Staib W, Broelsch C. Preparation of parenchymal and non-parenchymal cells from adult human liver - morphological and biochemical characteristics. J Clin Chem Clin Biochem 1976;14(11):527-532
4. Reese JA, Byard JL. Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 1981;17(11):935-940
5. Strom SC, Jirtle RL, Jones RS, Novicki DL, Rosenberg MR, Novotny A, Irons G, McLain JR, Michalopoulos G. Isolation, culture, and transplantation of human hepatocytes. J Natl Cancer Inst 1982;68(5):771-778
6. Guguen-Guillouzo C, Campion JP, Brissot P, Glaise D, Launois B, Bourel M, Guillouzo A. High yield separation of isolated human adult hepatocytes by enzymatic perfusion of the liver. Biochem Pharmacol 1982;31(14):2427-2430
7. Seglen PO, Reith A. Ammonia inhibition of protein degradation in isolated rat hepatocytes - quantitative ultrastructural alterations in the lysosomal system. Exp Cell Res 1976;100(2):276-280
8. Godoy P, Hewitt NJ, Luebke-Wheeler J, Gibson A, Eakins R, Goldring CEP, et al. Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol 2013; (in press)
9. Knobeloch D, Ehnert S, Schyschka L, Büchler P, Schoenberg M, Kleeff J, et al. Human hepatocytes: isolation, culture, and quality procedures. Methods Mol Biol 2012;806:99-120
10. Weiss TS, Pahernik SA, Scherübl I, Jauch KW, Thasler WE. Cellular damage to human hepatocytes through repeated application of 5-aminolevulinic acid. J Hepatol 2003;38(4):476-482
11. Weiss TS, Jahn B, Cetto M, Jauch KW, Thasler WE. Collagen sandwich culture affects intracellular polyamine levels of human hepatocytes. Cell Prolif 2002;35(5):257-267
12. Yoong KF, McNab G, Hübscher SG, Adams DH. Vascular adhesion protein-1 and ICAM-1 support the adhesion of tumor-infiltrating lymphocytes to tumor endothelium in human hepatocellular carcinoma. J Immunol 1998;160(8):3978-3988
13. Gerlach JC, Zeilinger K, Spatkowski G, Hentschel F, Schnoy N, Kolbeck S, et al. Large-scale isolation of sinusoidal endothelial cells from pig and human liver. J Surg Res 2001;100(1):39-45
14. Daneker GW, Lund SA, Caughman SW, Swerlick RA, Fischer AH, Staley CA, Ades EW. Culture and characterization of sinusoidal endothelial cells isolated from human liver. In Vitro Cell Dev Biol Anim 1998;34(5):370-377
15. Katayanagi K, Kono N, Nakanuma Y. Isolation, culture and characterization of biliary epithelial cells from different anatomical levels of the intrahepatic and extrahepatic biliary tree from a mouse. Liver 1998;18(2):90-98
16. Heydtmann M, Lalor PF, Eksteen JA, Hübscher SG, Briskin M, Adams DH. CXC chemokine ligand 16 promotes integrin-mediated adhesion of liver-infiltrating lymphocytes to cholangiocytes and hepatocytes within the infiltrated human liver. J Immunol 2005;174(2):1055-1062
17. Chiarini LB, Takiya CM, Borojevic R, Monteiro AN. Long-term culture of cholangiocytes from fibro-granulomatous lesions. BMC Gastroenterol 2006;55(12):1760-1767
18. Liu WH, Li R, Dou KF. Convenient and efficient enrichment of the CD133 + liver cells from rat fetal liver cells as a source of liver stem/progenitor cells. Stem Cell Rev Rep 2011;7(1):94-102
19. Si-Tayeb K, Lemaigre FP, Duncan SA. Organogenesis and development of the liver. Dev Cell 2010;18(2):175-189
20. Xu CS, Chen XG, Chang CF, Wang GP, Wang WB, Zhang LX, et al. Differential gene expression and functional analysis of pit cells from regenerating rat liver. Genet Mol Res 2011;10(2):678-692
21. Kupffer KW. Über Sternzellen der Leber. Arch Mikroskop Anat 1876;12:353-358
22. Gandhi CR. Kupffer cells; molecular pathology of liver diseases. Mol pathol library 2011;5:81-95
23. Page DT, Garvey JS. Isolation and characterization of hepatocytes and Kupffer cells. J Immunol Methods 1979;27(2):159-173
24. Friedman SL, Rocky DC, McGuire RF, Maher JJ, Boyles JK, Yamasaki G. Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture. Hepatology 1992;15:234-243
25. Froh M, Konno A, Thurman RG. Hepatotoxicology: isolation of liver Kupffer cells (basic protocol). Curr Protoc Toxicol 2000;14(4):1-12
26. Riccalton-Banks L, Bhandari R, Fry J, Shakesheff KM. A simple method for the simultaneous isolation of stellate cells and hepatocytes from rat liver tissue. Mol Cell Biochem 2003;248(1-2):97-102
27. Hellerbrand C. Hepatic stellate cells - the pericytes in the liver; Pflugers Arch. Eur J Physiol 2013;465(5):775-778.
28. Knook DL, Seffelaar AM, de Leeuw AM. Fat-storing cells of the rat liver - their isolation and purification. Exp Cell Res 1982;139(2):468-471
29. Olsen AL, Bloomer SA, Chan EP, Gaca MDA, Georges PC, Sackey B, et al. Hepatic stellate cells require a stiff environment for myofibroblastic differentiation. Am L Physiol Gastrointest Liver Physiol 2011;301(1):G110-G118
30. Elvevold K, Smedsrød B, Martinez I. The liver sinusoidal endothelial cell: a cell type of controversial and confusing identity. Am J Physiol Gastrointest Liver Physiol 2008;294(2):G391-G400
31. Braet F, Wisse E. Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review. Comp Hepatol 2002;1(1):1
32. Lalor PF, Lai WK, Curbishley SM, Shetty S, Adams DH. Human hepatic sinusoidal endothelial cells can be distinguished by expression of phenotypic markers related to their specialised functions in vivo. World J Gastroenterol 2006;12(34):5429-5439
33. Tokairin T, Nishikawa Y, Doi Y, Watanabe H, Yoshioka T, Su M, et al. A highly specific isolation of rat sinusoidal endothelial cells by the immunomagnetic bead method using SE-1 monoclonal antibody. J Hepatol 2002;36(6):725-733
34. Nonaka H, Tanaka M, Suzuki K, Miyajima A. Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors. Dev Dyn 2007;236(8):2258-2267
35. Alabraba EB, Curbishley SM, Lai WK, Wigmore SJ, Adams DH, Afford SC. A new approach to isolation and culture of human Kupffer cells. J Immunol Methods 2007;326(1-2):139-144
36. Tacke F, Weiskirchen R. Update on hepatic stellate cells: pathogenic role in liver fibrosis and novel isolation techniques. Expert Rev Gastoenterol Hepatol 2012;6(1):67-80
37. Friedman SL. Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiol Rev 2008;88(1):125-172
38. Neubauer K, Knittel T, Aurisch S, Fellmer P, Ramadori G. Glial fibrillary protein-a cell type specific marker for Ito cells in vivo and in vitro. J Hepatol 1996;24(6):719-730
39. Puppi J, Strom SC, Hughes RD, Bansal S, Castell JV, Dagher I, et al. Improving the techniques for human hepatocyte transplantation: report from a consensus meeting in London. Cell Transplant 2012;21(1):1-10