Purification of heterotrimeric G protein α subunits by GST-Ric-8 association: Primary characterization of purified Gαolf

Journal of Biological Chemistry

Volumn 286, Issue 4, 2011, Pages 2625-2635

  Chan, PuiYee ^a   Gabay, Meital ^a   Wright, Forrest A ^a   Kan, Wei ^a   Oner, Sukru S ^b   Lanier, Stephen M ^b   Smrcka, Alan V ^a   Blumer, Joe B ^b   Tall, Gregory G ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLYL CYCLASE; CO-EXPRESSION; EFFECTOR ENZYMES; G PROTEIN; GLUTATHIONES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; GUANOSINES; HEK293 CELLS; HETEROTRIMERIC G PROTEINS; INSECT CELLS; ISOFORMS; LYSATES; PROTEIN EXPRESSIONS; PROTEIN G; SIMPLIFIED METHOD; TRIPHOSPHATE; WHOLE CELL;

GEL PERMEATION CHROMATOGRAPHY; PURIFICATION;

PROTEINS;

ADENYLATE CYCLASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; RIC 8 PROTEIN; RIC 8A PROTEIN; RIC 8B PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; GEL FILTRATION; HUMAN; HUMAN CELL; INSECT CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; STOICHIOMETRY; WESTERN BLOTTING;

ADENYLATE CYCLASE; ANIMALS; BACULOVIRIDAE; ENZYME ACTIVATION; GENE EXPRESSION; GLUTATHIONE TRANSFERASE; GTP-BINDING PROTEIN ALPHA SUBUNITS; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; HEK293 CELLS; HUMANS; ISOENZYMES; RECOMBINANT FUSION PROTEINS; SPODOPTERA;

HEXAPODA;

EID: 78951480403     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.178897     Document Type: Article

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