Selective blocking of primary amines in branched polyethylenimine with biocompatible ligand alleviates cytotoxicity and augments gene delivery efficacy in mammalian cells

Colloids and Surfaces B: Biointerfaces

Volumn 115, Issue , 2014, Pages 79-85

  Tripathi, Sushil K ^a,c   Gupta, Niharika ^a   Mahato, Manohar ^a   Gupta, Kailash C ^a,b   Kumar, Pradeep ^a  

^a CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

^b CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

^c THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Buffering capacity; Cytotoxicity; Polyethylenimine; Pyridoxal hydrochloride; Transfection efficiency

Indexed keywords

AMINES; BIOCOMPATIBILITY; CYTOLOGY; CYTOTOXICITY; EFFICIENCY; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; LIPID BILAYERS; LIPOSOMES; MAMMALS; NUCLEIC ACIDS; POLYMER BLENDS;

BUFFERING CAPACITIES; NUCLEIC ACIDS DELIVERIES; POLYETHYLENIMINES; POSITIVE SURFACE CHARGE; PYRIDOXAL HYDROCHLORIDE; SPECTROSCOPIC CHARACTERIZATION; TARGET GENE EXPRESSION; TRANSFECTION EFFICIENCY;

MOLECULAR BIOLOGY;

AMINE; BRANCHED POLYETHYLENEIMINE; DNA; LIGAND; LIPOFECTAMINE; POLYETHYLENEIMINE; POLYMER; PYRIDOXYL POLYETHYLENEIMINE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; BIOMATERIAL; BUFFER; DEOXYRIBONUCLEASE I; GREEN FLUORESCENT PROTEIN; HEPARIN; LIPID;

ARTICLE; BIOCOMPATIBILITY; CELL MEMBRANE; COMPLEX FORMATION; CONTROLLED STUDY; CYTOTOXICITY; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; PRIORITY JOURNAL; SPECTROSCOPY; SURFACE CHARGE; CELL DEATH; CELL SURVIVAL; CHEMISTRY; DRUG EFFECTS; FLUORESCENCE; GENE TRANSFER; HELA CELL LINE; HEMOLYSIS; MCF 7 CELL LINE; METABOLISM; NUCLEASE PROTECTION ASSAY; PARTICLE SIZE; PLASMID; STATIC ELECTRICITY; SYNTHESIS; TITRIMETRY;

MAMMALIA;

AMINES; BIOCOMPATIBLE MATERIALS; BUFFERS; CELL DEATH; CELL SURVIVAL; DEOXYRIBONUCLEASE I; DNA; FLUORESCENCE; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HELA CELLS; HEMOLYSIS; HEPARIN; HUMANS; LIGANDS; LIPIDS; MCF-7 CELLS; NUCLEASE PROTECTION ASSAYS; PARTICLE SIZE; PLASMIDS; POLYETHYLENEIMINE; RNA, SMALL INTERFERING; STATIC ELECTRICITY; TITRIMETRY;

EID: 84890131076     PISSN: 09277765     EISSN: 18734367     Source Type: Journal    
DOI: 10.1016/j.colsurfb.2013.11.024     Document Type: Article

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