Detection of EPO injections using a rapid lateral flow isoform test Anti-doping Analysis

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 30, 2013, Pages 9685-9691

  Lonnberg M ^a   Lundby, C ^b  

^a UPPSALA UNIVERSITY   (Sweden)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Epoetin beta; Erythropoietin; IEF; WGA affinity chromatography

Indexed keywords

COMPETITIVE SPORTS; CONFIDENCE LIMIT; EPOETIN BETA; ERYTHROPOIETIN (EPO); IEF; INJECTION PERIOD; RECOMBINANT HUMAN ERYTHROPOIETIN (RHEPO); SCREENING PROCEDURES;

AFFINITY CHROMATOGRAPHY;

TESTING;

BIOLOGICAL MARKER; ERYTHROPOIETIN; RECOMBINANT PROTEIN;

ADULT; ARTICLE; BLOOD; DOPING; HUMAN; IMMUNOASSAY; MALE; METHODOLOGY; URINE; YOUNG ADULT;

ADULT; BIOLOGICAL MARKERS; DOPING IN SPORTS; ERYTHROPOIETIN; HUMANS; IMMUNOASSAY; MALE; RECOMBINANT PROTEINS; YOUNG ADULT;

EID: 84890128710     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-6997-8     Document Type: Article

References (31)

1. Lundby C, Robach P, Saltin B (2012) The evolving science of detection of 'blood doping'. Br J Pharmacol 165(5):1306-1315
2. Barroso O, Mazzoni I, Rabin O (2008) Hormone abuse in sports: the antidoping perspective. Asian J Androl 10(3):391-402
3. Lundby C, Robach P, Boushel R, Thomsen JJ, Rasmussen P, Koskolou M, Calbet JA (2008) Does recombinant human EPO increase exercise capacity by means other than augmenting oxygen transport? J Appl Physiol 105(2):581-587
4. Halstenson CE, Macres M, Katz SA, Schnieders JR, Watanabe M, Sobota JT, Abraham PA (1991) Comparative pharmacokinetics and pharmacodynamics of epoetin alfa and epoetin beta. Clin Pharmacol Ther 50(6):702-712
5. Dou P, Liu Z, He J, Xu JJ, Chen HY (2008) Rapid and high-resolution glycoform profiling of recombinant human erythropoietin by capillary isoelectric focusing with whole column imaging detection. J Chromatogr 1190(1-2):372-376
6. Wang H, Dou P, Lu C, Liu Z (2012) Immuno-magnetic beads-based extraction-capillary zone electrophoresis-deep UV laser-induced fluorescence analysis of erythropoietin. J Chromatogr 1246:48-54
7. Wide L, Bengtsson C (1990) Molecular charge heterogeneity of human serum erythropoietin. Br J Haematol 76(1):121-127
8. Wide L, Bengtsson C, Berglund B, Ekblom B (1995) Detection in blood and urine of recombinant erythropoietin administered to healthy men. Med Sci Sports Exerc 27(11):1569-1576
9. Lasne F, Martin L, Crepin N, de Ceaurriz J (2002) Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones. Anal Biochem 311(2):119-126
10. Kohler M, Ayotte C, Desharnais P, Flenker U, Lüdke S, Thevis M, Völker-Schänzer E, Schänzer W (2008) Discrimination of recombinant and endogenous urinary erythropoietin by calculating relative mobility values from SDS gels. Int J Sports Med 29(01):1-6
11. Reichel C, Kulovics R, Jordan V, Watzinger M, Geisendorfer T (2009) SDS-PAGE of recombinant and endogenous erythropoietins: benefits and limitations of the method for application in doping control. Drug Test Anal 1(1):43-50
12. Franco Fraguas L, Carlsson J, Lönnberg M (2008) Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins. J Chromatogr 1212(1-2):82-88
13. Lönnberg M, Andren M, Birgegard G, Drevin M, Garle M, Carlsson J (2012) Rapid detection of erythropoiesis-stimulating agents in urine and serum. Anal Biochem 420:101-114
14. Reichel C (2011) Recent developments in doping testing for erythropoietin. Anal Bioanal Chem 401(2):463-481
15. Dehnes Y, Lamon S, Lonnberg M (2010) Erythropoietin (EPO) immunoaffinity columns - a powerful tool for purifying EPO and its recombinant analogues. J Pharm Biomed Anal 53(4):1028-1032
16. Lönnberg M, Dehnes Y, Drevin M, Garle M, Lamon S, Leuenberger N, Quach T, Carlsson J (2010) Rapid affinity purification of erythropoietin from biological samples using disposable monoliths. J Chromatogr 1217(45):7031-7037
17. Lonnberg M, Bondesson U, Cormant F, Garcia P, Bonnaire Y, Carlsson J, Popot MA, Rollborn N, Rasbo K, Bailly-Chouriberry L (2012) Detection of recombinant human EPO administered to horses using MAIIA lateral flow isoform test. Anal Bioanal Chem 403(6):1619-1628
18. Ashenden M, Sharpe K, Garnham A, Gore CJ (2012) Evaluation of the MAIIA dipstick test to detect recombinant human erythropoietin in plasma. J Pharm Biomed Anal 67-68:123-128
19. Lundby C, Achman-Andersen NJ, Thomsen JJ, Norgaard AM, Robach P (2008) Testing for recombinant human erythropoietin in urine: problems associated with current anti-doping testing. J Appl Physiol 105(2):417-419
20. Lasne F, Martin L, Martin JA, de Ceaurriz J (2007) Isoelectric profiles of human erythropoietin are different in serum and urine. Int J Biol Macromol 41(3):354-357
21. Lamon S, Robinson N, Sottas P-E, Henry H, Kamber M, Mangin P, Saugy M (2007) Possible origins of undetectable EPO in urine samples. Clin Chim Acta 385(1-2):61-66
22. Lamon S, Martin L, Robinson N, Saugy M, Ceaurriz J, Lasne F (2009) Effects of exercise on the isoelectric patterns of erythropoietin. Clin J Sport Med 19(4):311-315
23. Beullens M, Delanghe JR, Bollen M (2006) False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise. Blood 107(12):4711-4713
24. Jelkmann W (2007) Novel erythropoietic agents: a threat to sportsmanship. Medicina Sportiva 11(2):32-42
25. Lönnberg M, Drevin M, Carlsson J (2008) Ultra-sensitive immunochromatographic assay for quantitative determination of erythropoietin. J Immunol Methods 339(2):236-244
26. Zorzoli M (2005) Blood monitoring in anti-doping setting. Recent Adv Doping Anal 13:255-264
27. Sharpe K, Ashenden MJ, Schumacher YO (2006) A third generation approach to detect erythropoietin abuse in athletes. Haematologica 91(3):356-363
28. Borno A, Aachmann-Andersen NJ, Munch-Andersen T, Hulston CJ, Lundby C (2010) Screening for recombinant human erythropoietin using [Hb], reticulocytes, the OFF(hr score), OFF(z score) and Hb(z score): status of the Blood Passport. Eur J Appl Physiol 109(3):537-543
29. Franz SE (2009) Erythropoiesis-stimulating agents: development, detection and dangers. Drug Test Anal 1(6):245-249
30. Macdougall IC, Ashenden M (2009) Current and upcoming erythropoiesis-stimulating agents, iron products, and other novel anemia medications. Adv Chronic Kidney Dis 16(2):117-130
31. Dehnes Y, Hemmersbach P (2011) Effect of single doses of methoxypolyethylene glycol-epoetin beta (CERA, Mircera) and epoetin delta (Dynepo) on isoelectric erythropoietin profiles and haematological parameters. Drug Test Anal 3(5):291-299