Rapid affinity purification of erythropoietin from biological samples using disposable monoliths

Journal of Chromatography A

Volumn 1217, Issue 45, 2010, Pages 7031-7037

  Lönnberg, Maria ^a,b   Dehnes, Yvette ^c   Drevin, Malin ^b   Garle, Mats ^d   Lamon, Severine ^e   Leuenberger, Nicolas ^e   Quach, Trikien ^b   Carlsson, Jan ^a,b  

^a UPPSALA UNIVERSITY   (Sweden)

^b MAIIA Diagnostics   (Sweden)

^c OSLO UNIVERSITY HOSPITAL   (Norway)

^d KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^e UNIVERSITY CENTER OF LEGAL MEDICINE   (Switzerland)

Author keywords

Affinity purification; Doping; EPO; Monolith; Post translational modifications

Indexed keywords

AFFINITY PURIFICATION; DOPING; EPO; MONOLITH; POST-TRANSLATIONAL MODIFICATIONS;

ANTIBODIES; BODY FLUIDS; ELECTROPHORESIS; HORMONES; MONOLITHIC INTEGRATED CIRCUITS; RECOVERY; ULTRAFILTRATION;

PURIFICATION;

CONTINUOUS ERYTHROPOIESIS RECEPTOR ACTIVATOR; ERYTHROPOIETIN; NOVEL ERYTHROPOIESIS STIMULATING PROTEIN; RECOMBINANT ERYTHROPOIETIN;

ADULT; ARTICLE; CONTROLLED STUDY; DISPOSABLE EQUIPMENT; DOPING; FEMALE; HUMAN; ISOELECTRIC FOCUSING; MALE; NORMAL HUMAN; PRIORITY JOURNAL; PRODUCT RECOVERY; PROTEIN PROCESSING; PROTEIN PURIFICATION; URINALYSIS;

ANIMALS; CHROMATOGRAPHY, AFFINITY; DOPING IN SPORTS; ERYTHROPOIETIN, RECOMBINANT; FEMALE; HORSES; HUMANS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; MALE; MICE; PROTEASE INHIBITORS; PROTEIN ISOFORMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; REAGENT KITS, DIAGNOSTIC; ULTRACENTRIFUGATION;

EQUIDAE;

EID: 77957865953     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.09.034     Document Type: Article

References (25)

1. Azarkan M., Huet J., Baeyens-Volant D., Looze Y., Vandenbussche G. J. Chromatogr. B 2007, 849:81.
2. Lasne F. J. Immunol. Methods 2001, 253:125.
3. Lasne F., de Ceaurriz J. Nature 2000, 405:635.
4. Lasne F., Martin L., Crepin N., de Ceaurriz J. Anal. Biochem. 2002, 311:119.
5. Beullens M., Delanghe J.R., Bollen M. Blood 2006, 107:4711.
6. Lasne F., Martin L., Martin J.A., de Ceaurriz J. Int. J. Biol. Macromol. 2007, 41:354.
7. Reichel C. J. Mass Spectrom. 2008, 43:916.
8. Khan A., Grinyer J., Truong S.T., Breen E.J., Packer N.H. Clin. Chim. Acta 2005, 358:119.
9. Guan F., Uboh C.E., Soma L.R., Birks E., Chen J., You Y., Rudy J., Li X. Anal. Chem. 2008, 80:3811.
10. Sytkowski A.J., Lunn E.D., Davis K.L., Feldman L., Siekman S. Proc. Natl. Acad. Sci. U.S.A. 1998, 95:1184.
11. Josic D., Clifton J.G. J. Chromatogr. A 2007, 1144:2.
12. Jungbauer A., Hahn R. J. Sep. Sci. 2004, 27:767.
13. Tennikova T.B., Svec F. J. Chromatogr. 1993, 646:279.
14. Thömmes J., Kula M.-R. Biotechnol. Progr. 1995, 11:357.
15. Rucevic M., Clifton J.G., Huang F., Li X., Callanan H., Hixson D.C., Josic D. J. Chromatogr. A 2006, 1123:199.
16. Lonnberg M., Drevin M., Carlsson J. J. Immunol. Methods 2008, 339:236.
17. Ayotte C., Gmeiner G., Lasne F., Pascual P.A., Reichel C., Saugy M. WADA Technical Document - TD2009EPO 2009.
18. Clerico A., Giammattei C., Cecchini L., Lucchetti A., Cruschelli L., Penno G., Gregori G., Giampietro O. Clin. Chem. 1990, 36:562.
19. Dehnes Y., Lamon S., Lönnberg M. J. Pharm. Biomed. Anal. 2010, 53(4):1028.
20. N. Leuenberger, manuscript in preparation.
21. Macdougall I.C. Curr. Hematol. Rep. 2005, 4:436.
22. Ho E.N., Wan T.S., Wong A.S., Lam K.K., Stewart B.D. J. Chromatogr. A 2008, 1201:183.
23. Kuuranne T., Thomas A., Leinonen A., Delahaut P., Bosseloir A., Schanzer W., Thevis M. Rapid Commun. Mass Spectrom. 2008, 22:355.
24. Thevis M., Thomas A., Delahaut P., Bosseloir A., Schanzer W. Anal. Chem. 2006, 78:1897.
25. Bidlingmaier M., Suhr J., Ernst A., Wu Z., Keller A., Strasburger C.J., Bergmann A. Clin. Chem. 2009, 55:445.